Immobilization of Pseudomonas aeruginosa FNCC-0063 on Calcium Alginate and Its Application as Bioabsorbent
Abstract
Biosorption of Cu (II) using bacteria Pseudomonas aeruginosa FNCC-0063 was immobilized on calcium alginate (PAI). This research examined the effect of various parameters such as pH, contact time, and initial Cu (II) concentration. The biosorption mechanism of Cu (II) was studied by sequential desorption with H2O, KNO3 1 M, HNO3 0.5 M and Na2EDTA 0.1 M. Cu (II) concentration was analyzed using atomic absorption spectrophotometer (AAS). The results showed that optimum Cu (II) ion biosorption occurred at a pH biosorption rate constant of 0.03724 g mg-1.min-1. The kinetics studies showed that Cu (II) biosorption follows pseudo-second-order. The biosorption capacities of 36.60 mg/g. Cu (II) Biosorption followed the Freundlich equation, as shown by a high correlation coefficient (R2) of about 0.99. Ionic bonds dominated the biosorption mechanism of Cu (II) ion on immobilized PAI.
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Y. A. El-Amier et al., “Assessment of the heavy metals pollution and ecological risk in sediments of mediterranean sea drain estuaries in egypt and phytoremediation potential of two emergent plants,” Sustainability (Switzerland), vol. 13, no. 21, 2021, doi: 10.3390/su132112244.
Z. Masood et al., “Monitoring pond water quality to improve the production of Labeo rohita (Hamilton, 1822) in Bannu Fish Hatchery of Bannu district, Khyber Pakhtunkhwa province; An Implications for artificial fish culture,” Brazilian Journal of Biology, vol. 83, 2023, doi: 10.1590/1519-6984.245197.
E. A. Pratidina, E. Suhartono, and B. Setiawan, “Impact of Heavy Metals on Hexokinase Isoforms: An In Silico Study,” Berkala Kedokteran, vol. 18, no. 1, 2022, doi: 10.20527/jbk.v18i1.12801.
M. Priessner et al., “Selective Detection of Cu+ Ions in Live Cells via Fluorescence Lifetime Imaging Microscopy,” Angewandte Chemie - International Edition, vol. 60, no. 43, 2021, doi: 10.1002/anie.202109349.
H. Yuan et al., “Co-exposure of sulfur nanoparticles and Cu alleviate Cu stress and toxicity to oilseed rape Brassica napus L.,” J Environ Sci (China), vol. 124, 2023, doi: 10.1016/j.jes.2021.09.040.
M. Werr, E. Kaifer, M. Enders, A. Asyuda, M. Zharnikov, and H. J. Himmel, “A Copper(I) Complex with Two Unpaired Electrons, Synthesised by Oxidation of a Copper(II) Complex with Two Redox-Active Ligands,” Angewandte Chemie - International Edition, vol. 60, no. 43, 2021, doi: 10.1002/anie.202109367.
C. Sukumar, V. Janaki, S. Kamala-Kannan, and K. Shanthi, “Biosorption of chromium(VI) using Bacillus subtilis SS-1 isolated from soil samples of electroplating industry,” Clean Technol Environ Policy, vol. 16, no. 2, 2014, doi: 10.1007/s10098-013-0636-0.
M. ben Amar, K. Walha, and V. Salvadó, “Valorisation of Pine Cone as an Efficient Biosorbent for the Removal of Pb(II), Cd(II), Cu(II), and Cr(VI),” Adsorption Science and Technology, vol. 2021, 2021, doi: 10.1155/2021/6678530.
A. Saravanan, P. S. Kumar, S. Jeevanantham, P. Harikumar, V. Bhuvaneswari, and S. Indraganti, “Identification and sequencing of bacteria from crop field: Application of bacteria — agro-waste biosorbent for rapid pesticide removal,” Environ Technol Innov, vol. 25, 2022, doi: 10.1016/j.eti.2021.102116.
H. Nh, K. Akli, R. Youfa, M. I. Senjawati, and M. Khairati, “Biosorption of Cu(II) metal ions in fixed column by using coconut husk waste,” Oriental Journal of Chemistry, vol. 34, no. 4, 2018, doi: 10.13005/ojc/3404062.
M. T. T. Thi, D. Wibowo, and B. H. A. Rehm, “Pseudomonas aeruginosa biofilms,” International Journal of Molecular Sciences, vol. 21, no. 22. 2020. doi: 10.3390/ijms21228671.
J. S. de Lima et al., “Immobilization of Aspergillus ficuum tannase in calcium alginate beads and its application in the treatment of boldo (Peumus boldus) tea,” Int J Biol Macromol, vol. 118, 2018, doi: 10.1016/j.ijbiomac.2018.07.084.
W. Zhao, T. Zhou, J. Zhu, X. Sun, and Y. Xu, “Adsorption of cadmium ions using the bioadsorbent of Pichia kudriavzevii YB5 immobilized by polyurethane foam and alginate gels,” Environmental Science and Pollution Research, vol. 25, no. 4, 2018, doi: 10.1007/s11356-017-0785-5.
M. Yasir et al., “Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa,” Front Microbiol, vol. 10, 2020, doi: 10.3389/fmicb.2019.03053.
B. Pérez-Cid, S. Calvar, A. B. Moldes, and J. Manuel Cruz, “Effective Removal of Cyanide and Heavy Metals from an Industrial Electroplating Stream Using Calcium Alginate Hydrogels,” Molecules, vol. 25, no. 21, 2020, doi: 10.3390/molecules25215183.
K. Sui et al., “Biocomposite fiber of calcium alginate/multi-walled carbon nanotubes with enhanced adsorption properties for ionic dyes,” Carbohydr Polym, vol. 90, no. 1, 2012, doi: 10.1016/j.carbpol.2012.05.057.
J. Kim, I. Hiltpold, G. Jaffuel, I. Sbaiti, B. E. Hibbard, and T. C. J. Turlings, “Calcium-alginate beads as a formulation for the application of entomopathogenic nematodes to control rootworms,” J Pest Sci (2004), vol. 94, no. 4, 2021, doi: 10.1007/s10340-021-01349-4.
A. Martínez-Arcos, M. Reig, J. M. Cruz, J. L. Cortina, A. B. Moldes, and X. Vecino, “Evaluation of calcium alginate-based biopolymers as potential component of membranes for recovering biosurfactants from corn steep water,” Water (Switzerland), vol. 13, no. 17, 2021, doi: 10.3390/w13172396.
K. M. Lee et al., “Preparation of calcium alginate-encapsulated sulfur particles and their application in metal nanoparticle capture: A case study of silver nanoparticles,” ScienceAsia, vol. 47, no. S1, 2021, doi: 10.2306/SCIENCEASIA1513-1874.2021.S006.
A. Rasheed et al., “Immobilization of Pseudomonas aeruginosa static biomass on eggshell powder for on-line preconcentration and determination of Cr (VI),” Open Chem, vol. 18, no. 1, 2020, doi: 10.1515/chem-2020-0031.
R. J. Nathan, C. E. Martin, D. Barr, and R. J. Rosengren, “Simultaneous removal of heavy metals from drinking water by banana, orange and potato peel beads: a study of biosorption kinetics,” Appl Water Sci, vol. 11, no. 7, 2021, doi: 10.1007/s13201-021-01457-7.
T. B. da Costa, M. G. C. da Silva, and M. G. A. Vieira, “Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies,” Int J Biol Macromol, vol. 165, 2020, doi: 10.1016/j.ijbiomac.2020.10.072.
I. Syauqiah, D. Nurandini, N. P. Prihatini, and Jamiyaturrasidah, “Determination of rice husk activated Carbon capacity in adsorption of Cu Metal from Sasirangan liquid waste based on isotherm model,” IOP Conf Ser Mater Sci Eng, vol. 1212, no. 1, 2022, doi: 10.1088/1757-899x/1212/1/012019.
I. E. Wijayanti and E. A. Kurniawati, “Studi Kinetika Adsorpsi Isoterm Persamaan Langmuir dan Freundlich pada Abu Gosok sebagai Adsorben,” EduChemia (Jurnal Kimia dan Pendidikan), vol. 4, no. 2, 2019, doi: 10.30870/educhemia.v4i2.6119.
L. Pescosolido et al., “In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications,” Acta Biomater, vol. 7, no. 4, 2011, doi: 10.1016/j.actbio.2010.11.040.
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