Isolation and Molecular Characterization of Gelatinase-Producing Bacteria from Mangrove Sediment
Abstract
Protease is an important enzyme widely produced by microorganisms applied in food, health, and industry. Mangrove ecosystem, a rich microorganism habitat, accounted as a new resource for isolating the proteolytic bacteria. The purpose of this study was to identify protease-producing bacteria from mangrove ecosystems in the Tuban area, Indonesia. Three isolates that produced the gelatinase was successfully isolated from mangrove sediments. Bacterial isolates were then tested for extracellular gelatinase. The results showed that isolate T1 had high gelatinase activity. Two isolates (isolates T2 and T3) produced moderately gelatinase enzymes. Molecular identification revealed that isolate T1 is Enterobacter hormaechei.
References
Adan A, Kiraz Y, Baran Y. 2016. Cell proliferation and cytotoxicity assays. Current pharmaceutical biotechnology. vol 17(14): 1213–1221.
Alexopoulos JA, Guarné A, Ortega J. 2012. ClpP: a structurally dynamic protease regulated by AAA+ proteins. Journal of structural biology. vol 179(2): 202–210. doi: https://doi.org/10.1016/j.jsb.2012.05.003.
Aranda PS, LaJoie DM, Jorcyk CL. 2012. Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis. vol 33(2): 366–369. doi: https://doi.org/10.1002/elps.201100335.
Balakrishnan V, Aarthi M, Eswaran P, Rajeshkumar MP. 2019. Isolation and Characterization of Gelatinase Producing Halophilic Bacteria from the Sediments of Pichavaram Mangrove Forest, Tamil Nadu State, South India. Research & Reviews: A Journal of Life Sciences. vol 9(3): 63–70.
Baldauf SL. 2003. Phylogeny for the faint of heart: a tutorial. Trends in Genetics. vol 19(6): 345–351. doi: https://doi.org/10.1016/S0168-9525(03)00112-4.
Chen Q, Zhao Q, Li J, Jian S, Ren H. 2016. Mangrove succession enriches the sediment microbial community in South China. Scientific reports. vol 6(27468): 1–9. doi: https://doi.org/10.1038/srep27468.
Clausen T, Southan C, Ehrmann M. 2002. The HtrA family of proteases: implications for protein composition and cell fate. Molecular cell. vol 10(3): 443-455. doi: https://doi.org/10.1016/S1097-2765(02)00658-5.
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. 2008. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research. vol 36(2): 465–469. doi: https://doi.org/10.1093/nar/gkn180.
Dias AC, Andreote FD, Rigonato J, Fiore MF, Melo IS, Araújo WL. 2010. The bacterial diversity in a Brazilian non-disturbed mangrove sediment. Antonie Van Leeuwenhoek. vol 98(4): 541–551. doi: https://doi.org/10.1007/s10482-010-9471-z.
Dilhari A, Sampath A, Gunasekara C, Fernando N, Weerasekara D, Sissons C, McBain A, Weerasekera M. 2017. Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method. AMB Express. vol 7(1): 1–11. doi: https://doi.org/10.1186/s13568-017-0477-z.
Gibbs DJ, Isa NM, Movahedi M, Lozano-Juste J, Mendiondo GM, Berckhan S, Rosa NML, Conde JV, Correia CS, Pearce SP, Bassel GW, Hamali B, Talloji P, Tomé DFA, Coego A, Beynon J, Alabadi D, Bachmair A, León J, Gray JE, Theodoulou FL, Holdsworth MJ. 2014. Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors. Molecular Cell. vol 53(3): 369–379. doi: https://doi.org/10.1016/j.molcel.2013.12.020.
Gomes NC, Cleary DF, Calado R, Costa R. 2011. Mangrove bacterial richness. Communicative & Integrative Biology. vol 4(4): 419-423. doi: https://doi.org/10.4161/cib.15253.
Guenther F, Maus D, Hedtrich S, Melzig MF. 2019. Serine Protease Mauritanicain from Euphorbia mauritanica and Phorbol-12-myristate-13-acetate Modulate the IL-8 Release in Fibroblasts and HaCaT Keratinocytes. Planta Medica. vol 85(7): 578–582. doi: https://doi.org/10.1055/a-0735-9911.
Gupta V, Singh PK, Korpole S, Tanuku NRS, Pinnaka AK. 2017. Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest Free. International Journal of Systematic and Evolutionary Microbiology. vol 67(7): 2219–2224. doi: https://doi.org/10.1099/ijsem.0.001928.
Haldar S, Nazareth SW. 2018. Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis. 3 Biotech. vol 8(436): 1-10. doi: https://dx.doi.org/10.1007%2Fs13205-018-1441-6.
Jabalia N, Mishra PC, Chaudhary N. 2014. Applications, challenges and future prospects of proteases: An overview. Journal of Agroecology and Natural Resource Management. vol 1(3): 179–183.
Kathiresan K, Saravanakumar K, Anburaj R, Gomathi V, Abirami G, Sahu SK, Anandhan S. 2011. Microbial enzyme activity in decomposing leaves of mangroves. International Journal of Advanced Biotechnology and Research. vol 2(3): 382–389.
Kumar S, Hatha AAM, Christi KS. 2007. Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags. Revista de Biología Tropical. vol 55(3–4): 777–786.
Lamkanfi M, Festjens N, Declercq W, Berghe TV, Vandenabeele P. 2007. Caspases in cell survival, proliferation and differentiation. Cell Death & Differentiation. vol 14(1): 44–55. doi: https://doi.org/10.1038/sj.cdd.4402047.
Langer T. 2000. AAA proteases: cellular machines for degrading membrane proteins. Trends in biochemical sciences. vol 25(5): 247–251. doi: https://doi.org/10.1016/S0968-0004(99)01541-8.
Luo L, Meng H, Wu RN, Gu JD. 2017. Impact of nitrogen pollution/deposition on extracellular enzyme activity, microbial abundance and carbon storage in coastal mangrove sediment. Chemosphere. vol 177: 275–283. doi: https://doi.org/10.1016/j.chemosphere.2017.03.027.
Matsushima Y, Goto YI, Kaguni LS. 2010. Mitochondrial Lon protease regulates mitochondrial DNA copy number and transcription by selective degradation of mitochondrial transcription factor A (TFAM). Proceedings of the National Academy of Sciences. vol 107(43): 18410–18415. doi: https://doi.org/10.1073/pnas.1008924107.
Mendes LW, Tsai SM. 2014. Variations of bacterial community structure and composition in mangrove sediment at different depths in Southeastern Brazil. Diversity. vol 6(4): 827–843. doi: https://doi.org/10.3390/d6040827.
Ochman H. 2005. Genomes on the shrink. Proceedings of the National Academy of Sciences. vol 102(34): 11959-11960.
Penna V, Lipay MV, Duailibi MT, Duailibi SE. 2015. The likely role of proteolytic enzymes in unwanted differentiation of stem cells in culture. Future science OA. vol 1(3): 1–7. doi: https://dx.doi.org/10.4155%2Ffso.15.26.
Pessoa TB, Rezende RP, Marques EDLS, Pirovani CP, dos Santos TF, dos Santos Gonçalves AC, Romano CC, Dotivo NC, Freitas ACO, Salay LC, Dias JCT. 2017. Metagenomic alkaline protease from mangrove sediment. Journal of Basic Microbiology. vol 57(11): 962–973. doi: https://doi.org/10.1002/jobm.201700159.
Poretsky R, Rodriguez-R LM, Luo C, Tsementzi D, Konstantindis KT. 2014. Strengths and limitations of 16s rRNA gene amplicon sequencing in revealing temporal microbial community dynamics. PloS one. vol 9(4): 1–12. doi: https://doi.org/10.1371/journal.pone.0093827.
Prihanto AA, Nursyam H. 2018. Screening and molecular identification of gelatinaseproducing bacteria isolated from Indonesian mangrove ecosystem. Asian Journal of Agriculture and Biology. vol 6(3): 316-320.
Rao CS, Sathish T, Ravichandra P, Prakasham R. 2009. Characterization of thermo-and detergent stable serine protease from isolated Bacillus circulans and evaluation of eco-friendly applications. Process Biochemistry. vol 44: 262–268. doi: 10.1016/j.procbio.2008.10.022.
Razzaq A, Shamsi S, Ali A, Ali Q, Sajjad M, Malik A, Ashraf M. 2019. Microbial proteases applications. Frontiers in bioengineering and biotechnology. vol 7(110): 1–20. doi: https://doi.org/10.3389/fbioe.2019.00110.
Rupali D. 2015. Screening and isolation of protease producing bacteria from soil collected from different areas of Burhanpur Region (MP) India. International Journal of Current Microbiology and Applied Science. vol 4(8): 597–606.
Salvesen GS, Hempel A, Coll NS. 2016. Protease signaling in animal and plant‐regulated cell death. The FEBS journal. vol 283(14): 2577–2598. doi: https://doi.org/10.1111/febs.13616.
Shahimi S, Mutalib SA, Khalid RM, Lamri MF, Ghani MA. 2019. Representative Candidate of Gelatinase Encoded Gene in Enterobacter aerogenes (Strain EA1) for Hydrolyzing Porcine Gelatin. Sains Malaysiana. vol 48(4): 773–780. doi: http://www.ukm.my/jsm/pdf_files/SM-PDF-48-4-2019/09%20Safiyyah%20Shahimi.pdf
Singh R, Kumar M, Mittal A, Mehta PK. 2016. Microbial enzymes: industrial progress in 21st century. 3 Biotech. vol 6(2): 1–15. doi: https://doi.org/10.1007/s13205-016-0485-8.
Singhal P, Nigam V, Vidyarthi A. 2012. Studies on production, characterization and applications of microbial alkaline proteases. International Journal of Advanced Biotechnology and Research. vol 3(3): 653–669.
Souza PMD, Bittencourt MLDA, Caprara CC, Freitas MD, Almeida RPCD, Silveira D, Fonseca YM, Filho EXF, Junior AP, Magalhães PO. 2015. A biotechnology perspective of fungal proteases. Brazilian Journal of Microbiology. vol 46(2): 337–346. doi: http://dx.doi.org/10.1590/S1517-838246220140359.
Thatoi H, Behera BC, Mishra RR, Dutta SK. 2013. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review. Annals of Microbiology. vol 63(1): 1–19. doi: https://doi.org/10.1007/s13213-012-0442-7.
Turk B, Turk D, Turk V. 2012. Protease signalling: the cutting edge. The EMBO journal. vol 31(7): 1630–1643. doi: https://dx.doi.org/10.1038%2Femboj.2012.42.
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