Beak Line and Eye Alignment as Phenotypic Sexing for Domestic Canaries (Serinus canaria)

  • Afif Muhammad Akrom
  • Soedarmanto Indarjulianto Universitas Gadjah Mada
    (ID)
  • Yanuartono Yanuartono
  • Trini Susmiati
  • Alfarisa Nururrozi
  • Slamet Raharjo
  • Rief Ghulam Satria Permana
  • Puveanthan Nagappan Govendan

Abstract

Phenotypic sexing of birds is a common practice among the songbird-keeping community, yet it is based on non-reputable information. This study aims to determine the sex of canaries (Serinus canaria) based on the alignment of the eye with the beak line. A total of 26 samples, consisting of six samples of one-month-old canaries (three males and three females based on PCR examination), 20 samples of six-month-old canaries (ten proven breeding pairs) were used in this study. The birds' heads were photographed from the sides, and then the positions of the eyes were compared with the shadow alignment of the beak. The results provided that five young birds and ten adult birds have a beak line alignment under the eye, while ten adult birds and one young bird have a beak line alignment across the eye. The accuracy of sexing using this method was 100% for both female and male adult canaries. However, this result could not be applied to sample that are not yet sexually mature, as two young male birds were found to have beak alignment below the eye. It can be concluded that sexing adult canaries can be performed by observing the eye and beak line's alignment.

References

Abdollahi H, Yeganeh HM, Shahrbabak HM. 2018. Sex determination in the canary based on the CHD gene located at the sex chromosome using feather. Iranian Journal of Animal Science. vol 49(2): 257–265. doi: http://doi:10.22059/ijas.2018.250993.653611.

Adkins-Regan E. 2007. Hormones and the development of sex differences in behaviour. Journal of Ornithology. vol 14891): 17–26. doi: http://doi: 10.1007/s10336-007-0188-3.

Agustina DP, Iriyanti N, Mugiyono S. 2013. Pertumbuhan dan konsumsi pakan pada berbagai jenis itik lokal betina yang pakannya di suplementasi probiotik. Jurnal Ilmiah Peternakan. vol 1(2): 691–698.

Asawakarn S, Teeranuwat I, Watcharaprapapong N, Siriwatchaiporn N, Somsai P, Kuldee M, Suriyaphol G, Dhitavat S. 2018. Comparison of dried blood spot, buccal swab, cloacal swab and feces as DNA sources to identify avian sexes by PCR. The Thai Journal of Veterinary Medicine. vol 48(3): 325–330.

Auzaini M, Mudawamah, Sunarto D, Fadli MZ. 2013. Variasi fenotipe morfometri burung kenari dewasa antara warna bulu terang kuning dan putih. Ternak Tropika Journal of Tropical Animal Production. vol 14(2): 31–37.

Baehaqi I, Saraswati TR, Yuniwarti EYW. 2018. Sex Determination in Male and Female Melopsittacus undulates using a Morphometric Method. Biosaintifika: Journal of Biology & Biology Education. vol 10(3): 533-538. doi: https://doi.org/10.15294/biosaintifika.v10i3.14067.

Caetano-Anolles K, Seo M, Rodriguez-Zas S, Oh JD, Han JY, Lee K, Park TS, Shin S, Jiao ZJ, Ghosh M, Jeong DK, Cho S, Kim H, Song KD, Lee HK. 2015. Comprehensive identification of sexual dimorphism-associated differentially expressed genes in two-way factorial designed RNA-Seq data on japanese quail (Coturnix coturnix japonica). PloS One. vol 10(9): 1–16. doi: https://doi.org/10.1371/journal.pone.0139324.

Çakmak E, Akın Pekşen Ç, Bilgin CC. 2017. Comparison of three different primer sets for sexing birds. Journal of Veterinary Diagnostic Investigation. vol 29(1): 59–63. doi: https://doi.org/10.1177%2F1040638716675197.

Cardoso GC, Hu Y, Mota PG. 2012. Birdsong, sexual selection, and the flawed taxonomy of canaries, goldfinches and allies. Animal Behaviour. vol 84(1): 111–119. doi: https://doi.org/10.1016/j.anbehav.2012.04.015.

Cerit H, Avanus K. 2007. Sex determination by CHDW and CHDZ genes of avian sex chromosomes in Nymphicus hollandicus. Turkish Journal of Veterinary and Animal Sciences. vol 31(6): 371–374.

Divers SJ. 2015. Endoscopic sex identification in chelonians and birds (Psittacines, Passerines, and Raptors). Veterinary Clinics: Exotic Animal Practice. vol 18(3): 541–554. doi: https://doi.org/10.1016/j.cvex.2015.05.006.

Doosti A, Fathpour H, Moshkelani S. 2009. Sex identification in the Canary using DNA typing methods. Bulgarian Journal of Veterinary Medicine. vol 12(3): 207–211.

Franz‐Odendaal TA, Krings M. 2019. A heterochronic shift in skeletal development in the barn owl (Tyto furcata): A description of the ocular skeleton and tubular eye shape formation. Developmental Dynamics. vol 248(8): 671–678. doi: https://doi.org/10.1002/dvdy.65.

Imamura T, Kawarada S, Sugimori F. 2015. A possible determination of age in the short-tailed shearwater Puffinus tenuirostris based upon tissue and the layered structure of the mandible. Journal of the Yamashina Institute for Ornithology. vol 47(1): 1–16 https://doi.org/10.3312/jyio.47.1.

Ishijima J, Uno Y, Nishida C, Matsuda Y. 2014. Genomic structures of the kW1 loci on the Z and W chromosomes in ratite birds: structural changes at an early stage of W chromosome differentiation. Cytogenetic and Genome Research. vol 142(4): 255–267. doi: https://doi.org/10.1159/000362479.

Julita U, Fitri LL, Fuadah YT. 2015. Kemampuan belajar bernyanyi pada burung kenari jantan muda (Serinus canaria Linn.) yang didedahkan secara live-tutoring dan tape-tutoring. Jurnal Istek. vol 9(1): 254–273.

Leboucher G, Vallet E, Nagle L, Béguin N, Bovet D, Hallé F, Ddraganoiu TI, Amy M, Kreutzer M. 2012. Studying female reproductive activities in relation to male song: the domestic canary as a model. Advances in the Study of Behavior. vol 44: 183-223. doi: https://doi.org/10.1016/B978-0-12-394288-3.00005-8.

Morinha F, Cabral JA, Bastos E. 2012. Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods. Theriogenology. vol 78(4): 703-714. doi: https://doi.org/10.1016/j.theriogenology.2012.04.015.

Mudawamah, Susilowati S, Trijaya. 2012. Phenotype variation of F1 crossbreed derived from black throat with local canary birds (Serinus canaria). Ternak Tropika Journal of Tropical Animal Production. vol 13(1): 1–8.

Padmadi V. 2010. Register yang digunakan dalam tabloid burung edisi januari 2010. [Skripsi]. Yogyakarta: Department of of Indonesian Language and Literature, Universitas Sanata Dharma.

Purwaningrum M, Nugroho HA, Asvan M, Karyanti K, Alviyanto B, Kusuma R, Haryanto A. 2019. Molecular techniques for sex identification of captive birds. Veterinary World. vol 12(9): 1506–1513. doi: http://doi:10.14202/vetworld.2019.1506-1513.

Qasimi MI, Mohibbi H, Nagaoka K, Watanabe G. 2018. Accumulation of steroid hormones in the eggshells of Japanese quail (Coturnix coturnix japonica). General and Comparative Endocrinology. vol 259: 161–164. doi: https://doi.org/10.1016/j.ygcen.2017.11.020.

Rallabandi HR, Yang H, Jo YJ, Lee HC, Byun SJ, Lee BR. 2019. Identification of female specific genes in the W chromosome that are expressed during gonadal differentiation in the Chicken. 한국가금학회지. vol 46(4): 287–296.

Roslinawati E, Prihatini W, Haryoko T. 2019. Variasi ciri morfometrik burung Bondol di Indonesia. Zoo Indonesia. vol 26(2): 116–129

Snyder KT, Creanza N. 2019. Polygyny is linked to accelerated birdsong evolution but not to larger song repertoires. Nature communications. vol 10(1): 1–15. doi: https://doi.org/10.1038/s41467-019-08621-3.

Vucicevic M, Stevanov‐Pavlovic M, Stevanovic J, Bosnjak J, Gajic B, Aleksic N, Stanimirovic Z. 2013. Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing. Zoo Biology. vol 32(3): 269–276. doi: https://doi.org/10.1002/zoo.21010.

Zein MSA, Haryoko T, Fitriana YS, Sulistyadi E, Prawiradilaga DM. 2017. Aplikasi kajian DNA molekuler dan fenotipik pada program pelepasliaran burung kakatua. Jurnal Biologi Indonesia. vol 13(1): 157–169. doi: http://dx.doi.org/10.14203/jbi.v13i1.3105.

Published
2020-06-30
Section
Research Articles
Abstract viewed = 568 times