Antifungal Activity of Morinda citrifolia leaf extracts against Colletotrichum acutatum
Abstract
Anthracnose is a plant disease that can infect a variety of plants worldwide. Fungal pathogen groups are the cause of anthracnose, one of which is Colletotrichum acutatum. Morinda citrifolia is famous for having antimicrobial activity. This study aims to determine the antifungal activity of M. citrifolia leaves extract against the growth of the fungus C. acutatum. The extract solvent used was 96% ethanol. The experiment consisted of five treatments (0%, 20%, 40%, 60% and propineb 0.1% as positive control). The method used in this study was the poisoned food technique. In this technique, C. acutatum was grown on PDA media mixed with M. citrifolia leaves extract. Antifungal activity was observed based on reduced mycelium growth compared to control. Observations were made every day by measuring the diameter of the fungus mycelium for eleven days. The analysis showed that 60% M. citrifolia leaves extract effectively inhibited the growth of the mycelium C. acutatum on the eleventh day of observation.
References
Almeida ÉS, de Oliveira D, Hotza D. 2019. Properties and applications of Morinda citrifolia (noni): a review. Comprehensive Reviews in Food Science and Food Safety. vol 18(4): 883‒909. doi: https://doi.org/10.1111/1541-4337.12456.
Anggreini S, Efri E, Nurdin M. 2016. Pengaruh tingkat konsentrasi fraksi ekstrak daun mengkudu dan mimba terhadap pertumbuhan dan sporulasi Colletotrichum Capsici. Jurnal Agrotek Tropika. vol 4(1): 43‒48. doi: http://dx.doi.org/10.23960/jat.v4i1.1899.
Assi RA, Darwis Y, Abdulbaqi IM, Vuanghao L, Laghari MH. 2017. Morinda citrifolia (Noni): A comprehensive review on its industrial uses, pharmacological activities, and clinical trials. Arabian Journal of Chemistry. vol 10(5): 691‒707. doi: https://doi.org/10.1016/j.arabjc.2015.06.018.
Bilal S, Ali L, Khan AL, Shahzad R, Asaf S, Imran M, Kang SM, Kim SK, Lee IJ. 2018. Endophytic fungus Paecilomyces formosus LHL10 produces sester-terpenoid YW3548 and cyclic peptide that inhibit urease and α-glucosidase enzyme activities. Archives of Microbiology. vol 200(10): 1493‒1502. doi: https://doi.org/10.1007/s00203-018-1562-7.
Bill M, Sivakumar D, Korsten L, Thompson AK. 2014. The efficacy of combined application of edible coatings and thyme oil in inducing resistance components in avocado (Persea americana Mill.) against anthracnose during post-harvest storage. Crop Protection. vol 64: 159‒167. doi: https://doi.org/10.1016/j.cropro.2014.06.015.
Cabardo Jr DE, Portugaliza HP. 2017. Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae. International Journal of Veterinary Science and Medicine. vol 5(1): 30‒34. doi: https://doi.org/10.1016/j.ijvsm.2017.02.001.
Coelho RT, Gonçalves-Vidigal MC, Vidigal Filho PS, Lacanallo GF, Darben LM, Silva CR,Sousa LL, Cruz AS. 2013. Characterization of the anthracnose resistance gene in the Mesoamerican common bean cultivar Crioulo 159. Annual Report of The Bean Improvement Cooperative. vol 56: 43-44.
Deng S, West BJ, Jensen CJ. 2011. Thermal degradation of flavonol glycosides in noni leaves during roasting. Advance Journal of Food Science and Technology. vol 3(2): 155‒159.
Gaffuri F, Longa CMO, Turchetti T, Danti R, Maresi G. 2017. ‘Pink rot’: infection of Castanea sativa fruits by Colletotrichum acutatum. Forest Pathology. vol 47(2): 1‒3. doi: https://doi.org/10.1111/efp.12307.
Giri IC, Qureshi MS, Khan SA, Patel J, Choudhary R, Singh A. 2010. Evaluation of the anthelmintic activity of Moringa oleifera seeds. International Journal of Pharma Professional's Research. vol 1(1): 88-89.
Goss M, Mafongoya P, Gubba A. 2017. Moringa oleifera extracts effect on Fusarium solani and Rhizoctonia solani Growth. Asian Research Journal of Agriculture. vol 6(1): 1‒10. doi: https://doi.org/10.9734/ARJA/2017/29835.
Hegazi AG, Megeed KNA, Hassan SE, Abdelaziz MM, Toaleb NI, El Shanawany EE, Aboelsoued D. 2018. Comparative ovicidal activity of Moringa oleifera leaf extracts on Fasciola gigantica eggs. Veterinary World. vol 11(2): 215‒220. doi: https://dx.doi.org/10.14202%2Fvetworld.2018.215-220.
Inel GA, Ungureau EM, Varley TS, Hirani M, Holt KB. 2016. Solvent–surface interactions between nanodiamond and ethanol studied with in situ infrared spectroscopy. Diamond and Related Materials. vol 61: 7‒13. doi: https://doi.org/10.1016/j.diamond.2015.11.001.
Ibrahim R, Hidayat SH, Widodo W. 2017. Keragaman morfologi, genetika, dan patogenisitas Colletotrichum acutatum penyebab antraknosa cabai di Jawa dan Sumatera. Jurnal Fitopatologi Indonesia. vol 13(1): 9‒16. doi: https://doi.org/10.14692/jfi.13.1.9.
Iskarlia GR, Rahmawati L, Chasanah U. 2014. Fungisida nabati dari tanaman serai wangi (Cymbopogon nardus) untuk menghambat pertumbuhan jamur pada batang karet (Hevea brasillensis Mueli, Arg). Polhasains: jurnal sains dan terapan Politeknik Hasnur. vol 3(1): 1-7.
Jalianto J. 2015. Uji aktivitas antijamur ekstrak etanol biji buah langsat (Lansium domesticum Corr.) terhadap jamur Candida albicans secara in vitro. [Dissertation]. Pontianak: Fakultas Kedokteran, Universitas Tanjungpura
Jayaraman SK, Manoharan MS, Illanchezian S. 2008. Antibacterial, antifungal and tumor cell suppression potential of Morinda citrifolia fruit extracts. International Journal of Integrative Biology. vol 3(1): 44-49.
Katata-Seru L, Moremedi T, Aremu OS, Bahadur I. 2018. Green synthesis of iron nanoparticles using Moringa oleifera extracts and their applications: removal of nitrate from water and antibacterial activity against Escherichia coli. Journal of Molecular Liquids. vol 256: 296-304. doi: https://doi.org/10.1016/j.molliq.2017.11.093.
Marcos JF, Gandía M, Harries E, Carmona L, Muñoz A. 2012. Antifungal peptides: exploiting non-lytic mechanisms and cell penetration properties. In Small wonders: peptides for disease control. Washington DC: American Chemical Society. vol 1095: 337-357. doi: 10.1021/bk-2012-1095.ch016.
Mari M, Guidarelli M, Martini C, Spadoni A. 2012. First report of Colletotrichum acutatum causing bitter rot on apple in Italy. Plant Disease. vol 96(1): 144‒144. doi: https://doi.org/10.1094/PDIS-06-11-0483.
Muñoz-Leoz B, Garbisu C, Antigüedad I, Ruiz-Romera E. 2012. Fertilization can modify the non-target effects of pesticides on soil microbial communities. Soil Biology and Biochemistry. vol 48: 125‒134. doi: https://doi.org/10.1016/j.soilbio.2012.01.021.
Oros G. 2010. Differential responses of Plasmopara halstedii developmental forms to various steroid alkaloids. International Journal of Life Sciences. vol 4: 1‒15. doi: https://doi.org/10.3126/ijls.v4i0.2791.
Peixoto JRO, Silva GC, Costa RA, Vieira GHF, Fonteles Filho AA, dos Fernandes Vieira RHS. 2011. In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts. Asian Pacific Journal of Tropical Medicine. vol 4(3): 201‒204. doi: https://doi.org/10.1016/S1995-7645(11)60069-2.
Rao A, Zhang Y, Muend S, Rao R. 2010. Mechanism of antifungal activity of terpenoid phenols resembles calcium stress and inhibition of the TOR pathway. Antimicrobial Agents and Chemotherapy. vol 54(12): 5062‒5069. doi: https://doi.org/10.1128/AAC.01050-10.
Ratnoglik SL, Aoki C, Sudarmono P, Komoto M, Deng L, Shoji I, Fuchino H, Kawahara N, Hotta H. 2014. Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus. Microbiology and Immunology. vol 58(3): 188‒194. doi: https://doi.org/10.1111/1348-0421.12133.
Septiadi T, Pringgenies D, Radjasa OK. 2013. Uji fitokimia dan aktivitas antijamur ekstrak teripang keling (Holoturia atra) dari pantai Bandengan Jepara terhadap jamur Candida albicans. Journal of Marine Research. vol 2(2): 76‒84. doi: https://doi.org/10.14710/jmr.v2i2.2355.
Silva JCE, Mourão DDSC, Lima FSDO, Sarmento RDA, Dalcin MS, Aguiar RWDS, Santos GRD. 2017. The efficiency of noni (Morinda citrifolia L.) essential oil on the control of leaf spot caused by Exserohilum turcicum in maize culture. Medicines. vol 4(3): 1‒10. doi: https://doi.org/10.3390/medicines4030060.
Tao N, Jia L, Zhou H. 2014. Anti-fungal activity of Citrus reticulata Blanco essential oil against Penicillium italicum and Penicillium digitatum. Food Chemistry. vol 153: 265-271. doi: https://doi.org/10.1016/j.foodchem.2013.12.070.
Veloso RA, de Souza Ferreira TP, Dias BL, Mourão DDSC, de Araújo Filho RN, Glória RSL, Barros AM, de Souza Ferreira TP, Chapla VM, Cangussu ASR, Machado SDCS, dos Santos GR. 2020. Chemical composition and bioactivity of essential oil from Morinda citrifolia L. fruit. Journal of Medicinal Plants Research. vol 14(5): 208-214. doi: https://doi.org/10.5897/JMPR2019.6853.
Vrhovsek A, Gereben O, Jamnik A, Pusztai L. 2011. Hydrogen bonding and molecular aggregates in liquid methanol, ethanol, and 1-propanol. The Journal of Physical Chemistry B. vol 115(46): 13473‒13488. doi: https://doi.org/10.1021/jp206665w.
Wang J, Qin X, Chen Z, Ju Z, He W, Tan Y, Zhou X, Tu Z, Lu F, Liu Y. 2016. Two new anthraquinones with antiviral activities from the barks of Morinda citrifolia (Noni). Phytochemistry Letters. vol 15: 13‒15. doi: https://doi.org/10.1016/j.phytol.2015.11.006.
Wijaya H, Novitasari N, Jubaidah S. 2018. Perbandingan metode ekstraksi terhadap rendemen ekstrak daun rambai laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung. vol 4(1): 79-83.
Zhang WM, Wang W, Zhang JJ, Wang ZR, Wang Y, Hao WJ, Huang WY. 2016. Antibacterial constituents of Hainan Morinda citrifolia (noni) leaves. Journal of Food Science. vol 81(5): M1192-M1196. doi: https://doi.org/10.1111/1750-3841.13302.
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