Modification of media compositions for micropropagation of Acorus calamus L.
Abstract
Modification of medium composition is a critical step in tissue culture to find out the best medium formulation for micropropagation. Furthermore, reduction of some nutrients as well as sugar and plant growth regulator concentrations are an effort often done to make the production of seedlings more economic. The aim of the study was to modify media composition by reduction of macronutrients and sugar concentrations in combination with BAP for micropropagation of Acorus calamus. Experiments of Calamus micropropagation was established by culturing single shoots to MS medium with 100, 50, 33.3 and 25% macronutrients added with sugar at 30, 20, and 10 g/l in combination with 0, 0.5, and 1 mg/l BAP. Each medium treatment had nine replicates. Growth of culture was determined every week until 6 weeks of culture. Growth variable recorded was shoots numbers, leaf length, leaf numbers and root numbers. At six weeks of culture, chlorophyll content was also observed. The results showed that reduction of macronutrients and sugar did not influence shoot and leaf numbers in MS medium but required 0.5-1 mg/l BAP. Rooting is best in medium with no addition of BAP. Chlorophyll content decreased in the medium containing BAP in all reduced macronutrients and sugar concentrations. All plantlets survived in the field. Reduction of macronutrients and sugar can be applied for Calamus shoot culture. Cytokinin BAP is critical for shoot growth but this could be omitted for rooting. Therefore, this finding offered more choices in micropropagation of Calamus with low-cost production.
Copyright (c) 2023 Aida Wulansari, Tri Muji Ermayanti, Erwin Al Hafiizh, Betalini Widhi Hapsari, Evan Maulana
This work is licensed under a Creative Commons Attribution 4.0 International License.
COPYRIGHT AND LICENSE STATEMENT
COPYRIGHT
Biogenesis: Jurnal Ilmiah Biologi is published under the terms of the Creative Commons Attribution license. Authors hold the copyright and retain publishing rights without restriction to their work. Users may read, download, copy, distribute, and print the work in any medium, provided the original work is properly cited.
LICENSE TO PUBLISH
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on http://creativecommons.org/licenses/by/4.0.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons Attribution license, the users are free to download, reuse, reprint, modify, distribute and/or copy the content for any purpose, even commercially, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
4. Co-Authorship
If the article was prepared jointly with other authors, the corresponding author warrants that he/she has been authorized by all co-authors, and agrees to inform his/her co-authors of the terms of this statement.
5. Miscellaneous
Biogenesis: Jurnal Ilmiah Biologi may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers.
