Extract of Dragon Fruit Pulp (Hylocereus polyrhizus) Potentially Stain Chromosomes of Red Onion (Allium ascalonicum)
Abstract
Side effect of aceto-orcein as a dye of chromosome structures remains some risks. This dye may remain tissue damage on eyes mucous membrane, burnt by skin contact, severe irritation of respiratory by spray mist. Exploration of natural dye is an alternative solution. Betacyanine of red dragon fruits Hylocereus polyrhizus pulp might have a potential role to stain chromosome. Aim of this study is to extract dragon fruit pulp to stain chromosomes of mitotic cells of red onion Allium ascalonicum. The method used in this research is qualitative description of application squash method with aceto-orcein as synthetic dye and two ratio of extract ratio of red dragon fruits pulps as natural dye to mitotic cells of red onion roots. The result revealed that 2:1 ratio of red dragon fruits and distilled water could stain chromosomes of red onion somatic cells. Further specific solvent is required to further investigation to get best betacyanine extract of red dragon fruits pulps.
References
Anjali M, and Srivastava AK. 2012. Karyological studies in twelve accessions of Carthamus tinctoriusi. Caryologia. vol 65(1): 1–6. https://doi.org/10.1080/00087114.2012.678072.
Armitage BA. 2005. Cyanine dye-DNA interactions: Intercalation, groove binding, and aggregation. Topics in Current Chemistry. vol 253: 55–76. https://doi.org/10.1007/b100442.
Asensio A, Kobko N, Dannenberg JJ. 2003. Cooperative hydrogen-bonding in adenine-thymine and guanine-cytosine base pairs. Density functional theory and møller-plesset molecular orbital study. Journal of Physical Chemistry A. vol 107(33): 6441–6443. https://doi.org/10.1021/jp0344646.
Cour LLa. 2009. Acettc-Orcein: A New Stain-Fixative for Chromosomes. Stain Technology. vol 16(4): 169–174. https://doi.org/https://doi.org/10.3109/10520294109107302.
Elgubbi H, and Asayh EA. 2007. Chromosome Staining with Natural Dyes from Punica granatum and Beta vulgaris. EC Nutrition. vol 11(4): 142–146.
Gianfranco V, Ravalli C, Cremonini R. 2008. The karyotype as a tool to identify plant species: Vicia species belonging to vicia subgenus. Caryologia. vol 61(3): 300-319.https://doi.org/10.1080/00087114.2008.10589642.
Gilbert KG, and Cooke DT. 2001. Dyes from plants: Past usage, present understanding and potential. Plant Growth Regulation. vol 34(1): 57–69. https://doi.org/10.1023/A:1013374618870.
Gorb L, Podolyan Y, Dziekonski P, Sokalski WA, Leszczynski J. 2004. Double-proton transfer in adenine-thymine and guanine-cytosine base pairs. A post-Hartree-Fock ab initio study. Journal of the American Chemical Society. vol 126(32): 10119–10129. https://doi.org/10.1021/ja049155n.
K, HP, Lunggani AT, Nurhakim MA. 2014. Chromosomes and Mitotic Cell Division Phase in Onion Roots After 24 Hours Acetoorcein Soaking Time. Bioma. vol 2(46-48): https://doi.org/https://doi.org/10.14710/bioma.14.2.46-48.
Karlsson HJ, Eriksson M, Perzon E, Åkerman B, Lincoln P, Westman G. 2003. Groove-binding unsymmetrical cyanine dyes for staining of DNA: Syntheses and characterization of the DNA-binding. Nucleic Acids Research. vol 31(21): 6227–6234. https://doi.org/10.1093/nar/gkg821.
Kasiri MB, and Safapour S. 2015. Exploring and Exploiting Plants Extracts as the Natural Dyes/Antimicrobials in Textiles Processing. Progress in Color, Colorants and Coatings. vol 8: 87-114.
Lysak MA, Fransz PF, Ali HBM, Schubert I. 2001. Chromosome painting in Arabidopsis thaliana. Plant Journal. vol 28(6): 689–697. https://doi.org/10.1046/j.1365-313x.2001.01194.x.
Maheswari PU, and Palaniandavar M. 2004. DNA binding and cleavage properties of certain tetrammine ruthenium(II) complexes of modified 1,10-phenanthrolines-Effect of hydrogen-bonding on DNA-binding affinity. Journal of Inorganic Biochemistry. vol 98(2): 219–230. https://doi.org/10.1016/j.jinorgbio.2003.09.003.
Maluszynska J. 2003. Cytogenetic tests for ploidy level analyses-chromosome counting. Doubled Haploid Production in Crop Plants. Dordrecht: Springer. https://doi.org/10.1007/978-94-017-1293-4_51.
MCCSD. 2009. Aceto-Orcein Solution, 2%. New York: Middle Country Central School District.
Muhlisyah N, Muthiadin C, Wahidah BF, Aziz, IR. 2014. Preparasi Kromosom Fase Mitosis Markisa Ungu (Passiflora edulis) Varietas Edulis Sulawesi Selatan. Biogenesis: Jurnal Ilmiah Biologi. vol 2(1): 48–55. https://doi.org/https://doi.org/10.24252/bio.v2i1.467.
Nafisi S, Saboury AA, Keramat N, Neault JF, Tajmir-Riahi HA. 2007. Stability and structural features of DNA intercalation with ethidium bromide, acridine orange and methylene blue. Journal of Molecular Structure. vol 827(1–3): 35–43. https://doi.org/10.1016/j.molstruc.2006.05.004.
Nurwanti M, Budiono JD, P RP. 2013. Pemanfaatan Filtrat Daun Muda Jati Sebagai Bahan Pewarna Alternatif Dalam Pembuatan Preparat Jaringan Tumbuhan. BioEdu. vol 2(1): 73–76.
Pan Y, Shi S, Gong X, Kuroda C. 2008. A natural hybrid between Ligularia paradoxa and L. duciformis (Asteraceae, Senecioneae) from Yunnan, China. Annals of the Missouri Botanical Garden. vol 95(3): 487–494. Retrieved from https://www.jstor.org/stable/25488207.
Ramesh A. 2015. Karyotypic analysis in three species of Allium and their some Varieties. International Research Journal of Biological Sciences. vol 4(9): 1–9.
Rebecca OPS, Boyce AN, Chandran S. 2010. Pigment identification and antioxidant properties of red dragon fruit (Hylocereus polyrhizus). African Journal of Biotechnology. vol 9(10): 1450–1454. https://doi.org/10.5897/AJB09.1603.
Tanaka Y, Sasaki N, Ohmiya A. 2008. Biosynthesis of plant pigments: Anthocyanins, betalains and carotenoids. Plant Journal. vol 54(4): 733–749. https://doi.org/10.1111/j.1365-313X.2008.03447.x.
Tenore GC, Novellino E, Basile A. 2012. Nutraceutical potential and antioxidant benefits of red pitaya (Hylocereus polyrhizus) extracts. Journal of Functional Foods. vol 4(1): 129–136. https://doi.org/10.1016/j.jff.2011.09.003.
Woo KK, Ngou FH, Ngo LS, Soong WK, Tang PY. 2011. Stability of betalain pigment from red dragon fruit (Hylocereus polyrhizus). American Journal of Food Technology. vol 6(2): 140–148. https://doi.org/10.3923/ajft.2011.140.148.
Yan X, Habbersett RC, Cordek JM, Nolan JP, Yoshida TM, Jett JH, Marrone BL. 2000. Development of a mechanism-based, DNA staining protocol using SYTOX orange nucleic acid stain and DNA fragment sizing flow cytometry. Analytical Biochemistry. vol 286(1): 138–148. https://doi.org/10.1006/abio.2000.4789.
Zipper H, Brunner H, Bernhagen J, Vitzthum F. 2004. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Research. vol 32(12): 1–10. https://doi.org/10.1093/nar/gnh101.
Copyright (c) 2018 Genesiska Genesiska, Herda Pratiwi
This work is licensed under a Creative Commons Attribution 4.0 International License.
COPYRIGHT AND LICENSE STATEMENT
COPYRIGHT
Biogenesis: Jurnal Ilmiah Biologi is published under the terms of the Creative Commons Attribution license. Authors hold the copyright and retain publishing rights without restriction to their work. Users may read, download, copy, distribute, and print the work in any medium, provided the original work is properly cited.
LICENSE TO PUBLISH
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on http://creativecommons.org/licenses/by/4.0.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons Attribution license, the users are free to download, reuse, reprint, modify, distribute and/or copy the content for any purpose, even commercially, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
4. Co-Authorship
If the article was prepared jointly with other authors, the corresponding author warrants that he/she has been authorized by all co-authors, and agrees to inform his/her co-authors of the terms of this statement.
5. Miscellaneous
Biogenesis: Jurnal Ilmiah Biologi may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers.
