Potensi Propolis Sebagai Imunomodulator Pada Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar yang diinduksi Penisilin-G
Abstract
Propolis is a honeybee product collected from plants sap. This resinous substance is used to build and maintain the nest. Propolis has long been declared as a safe material for health maintaining. The purpose of this research was to study the potential use of Propolis as an immunomodulator in Penicillin-G-induced Wistar rats. A total of 30 male rats aged 5 months with 200-240 grams body weight were divided into 6 groups: Control Placebo (im injection of aquabidest+oral administration of NaCl 0.9%); Control Penicillin (im injection of 21.6 mg Penicillin+oral administration of NaCl 0.9%); Control Propolis (im injection of aquabidest+oral administration of 27.0 mg Propolis); Treatment 1 (im injection of 21.6 mg Penicillin+oral administration of 13.5 mg Propolis); Treatment 2 (im injection of 21.6 mg Penicillin+oral administration of 27.0 mg Propolis); and Treatment 3 (im injection of 21.6 mg Penicillin+oral administration of 40.5 mg Propolis). Leukocytes profile (total and differential counts), body temperature, and urine acidity (pH) before and after treatment were determined as pretest and posttest data. Data were analyzed statistically using ANOVA followed by DMRT (p <0.05). Results showed that administration of Propolis significantly increased neutrophils count above the normal range (more than 4.41x103/L), whereas significantly decreased lymphocytes count below the normal range (less than 6.75x103/L). This causes the value of N/L ratio increased significantly. Administration of Penicillin and the combination with Propolis significantly increased body temperature (above 38C), while treatment with Propolis alone did not induce fever or inflammation (normal temperature 37-38C). Administration of Propolis reduced urine acidity due to the impact of the administration of Penicillin (pH 5.5-7.0). It can be concluded that oral administration of Propolis in Penicillin G-induced male Wistar rats exhibited immunosuppressive effect on lymphocytes. It was suggested as the role of Caffeic acid phenethyl ester (CAPE), the main bioactive compound in Propolis which also acts as an antiinflammatory agent. Further research is still needed to support this finding.
Keywords: CAPE, immune system, immunomodulators, Penicillin, Propolis
Copyright (c) 1970 Laksmindra Fitria, Onny Nurrahman Marwayana
This work is licensed under a Creative Commons Attribution 4.0 International License.
COPYRIGHT AND LICENSE STATEMENT
COPYRIGHT
Biogenesis: Jurnal Ilmiah Biologi is published under the terms of the Creative Commons Attribution license. Authors hold the copyright and retain publishing rights without restriction to their work. Users may read, download, copy, distribute, and print the work in any medium, provided the original work is properly cited.
LICENSE TO PUBLISH
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on http://creativecommons.org/licenses/by/4.0.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons Attribution license, the users are free to download, reuse, reprint, modify, distribute and/or copy the content for any purpose, even commercially, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
4. Co-Authorship
If the article was prepared jointly with other authors, the corresponding author warrants that he/she has been authorized by all co-authors, and agrees to inform his/her co-authors of the terms of this statement.
5. Miscellaneous
Biogenesis: Jurnal Ilmiah Biologi may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers.
