Larvicidal Efficacy of Citrus amblycarpa Peel Extract Against the Larvae of Aedes albopictus

  • Kasman Kasman Bagian Epidemiologi, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin
    (ID) http://orcid.org/0000-0002-6034-9808
  • Nuning Irnawulan Ishak Bagian Kesehatan Kerja dan Kesehatan Lingkungan, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin
    (ID)
  • Elsi Setiandari Lely Octaviana Bagian Administrasi dan Kebijakan Kesehatan, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin
    (ID)
  • Muhammad Rasyid Ridha Bagian Entomologi, Balai Penelitian dan Pengembangan Kesehatan Tanah Bumbu, Batulicin
    (ID)
Keywords: aedes albopictus, citrus amblycarpa, dengue fever, field test, larvicide

Abstract

Various ways to control dengue vector One of them is by using natural insecticides. The purpose of this study was to test the effectiveness of lime peel powder (Citrus amblycarpa) as an electric mosquito repellent against the death of Aedes albopictus mosquitoes in the field larvae. This study uses a true experimental design conducted at the Tanah Bumbu Health Research and Development Center. The object of this research was lime peel powder (Citrus amblycarpa) which was used as a natural insecticide against the Aedes albopictus mosquito. The larvae used were Aedes aegypti larvae instar III which were hatched at the Research and Development Center of P2B2 Tanah Bumbu and the results of larval collection using ovitrap in the Martapura Health Center Area 1. Susceptibility test for larval mortality and data collection by counting the number of dead Aedes albopictus larvae after being exhibited with skin extract lime for 24 hours at concentrations of 2%, 3%, 4%, and 5%. The LT50 mortality of larvae with lime extract was 2.78 hours (5%), faster than the mortality of larvae in the control group using abate. The results showed the potential of Limau Kuit peel extract as a larvicide in controlling the dengue vector.

kasman.jpg

Downloads

Download data is not yet available.

Author Biographies

Kasman Kasman, Bagian Epidemiologi, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin

Departemen Epidemiologi FKM Universitas Islam Kalimantan MAB Banjarmasin

Nuning Irnawulan Ishak, Bagian Kesehatan Kerja dan Kesehatan Lingkungan, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin

Departemen Kesehatan Lingkungan Dan Keselamatan Kerja Fakultas Kesehatan Masyarakat Universitas Islam Kalimantan MAB Banjarmasin

Elsi Setiandari Lely Octaviana, Bagian Administrasi dan Kebijakan Kesehatan, Universitas Islam Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin

Departemen AKK Fakultas Kesehatan Masyarakat Universitas Islam Kalimantan MAB Banjarmasin

Muhammad Rasyid Ridha, Bagian Entomologi, Balai Penelitian dan Pengembangan Kesehatan Tanah Bumbu, Batulicin

Balai Litbang Kesehatan Tanah Bumbu, Kalimantan Selatan

References

Adayani, W. S., Subahar, R., Fatmawaty, & Slamet. (2018). Effect of treatment using Carica papaya seed extract with Ag–TiO2 nanocomposite on the mortality of Aedes aegypti larvae. Journal of Physics: Conference Series, 1–9. https://doi.org/10.1088/1742-6596/1073/3/032035

Almadiy, A. A. (2020). Chemical composition, insecticidal and biochemical effects of two plant oils and their major fractions against Aedes aegypti, the common vector of dengue fever. Heliyon, 6(9), 1–9. https://doi.org/10.1016/j.heliyon.2020.e04915

Astriani, Y., & Widawati, M. (2016). Potensi Tanaman Di Indonesia Sebagai Larvasida Alami Untuk Aedes aegypti Potential Plant In Indonesia As Natural Larvicides For Aedes aegypti. SPIRAKEL, 8(2), 37–46. https://www.researchgate.net/profile/Mutiara-Widawati/publication/

Bhatt, S., Gething, P. W., Brady, O. J., Messina, J. P., Farlow, A. W., Moyes, C. L., … Hay, S. I. (2013). The global distribution and burden of dengue. Nature, 496(7446), 504–507. https://doi.org/10.1038/nature12060

Dinas Kesehatan Kabupaten Banjar. (2019). Kasus Demam Berdarah Dengue. Martapura.

Dita, N. T. W. S. (2015). Efektivitas Air Perasan Kulit Jeruk Manis sebagai Larvasida Nyamuk Aedes aegypti Effectivity of Sweet Orange Peel Juice as a Larvasides of Aedes aegypti Mosquito. Jurnal Kesehatan Masyarakat Nasional, 9(3), 207–213. http://dx.doi.org/10.21109/kesmas.v9i3.566

Elviani, E., Lucky, H., & Sardjito, E. W. (2019). Larvitrap Tipe Sekat dengan Nyamuk Aedes yang Terjebak. Thesis, Poltekkes Kemenkes Yogyakarta, 53(9), 1689–1699. http://eprints.poltekkesjogja.ac.id/876/

Francine, T. N., Prosper Cabral, B. N., Constant Anatole, P., Bruno, M. M., Pauline, N., & Jeanne, N. Y. (2016). Larvicidal activities of hydro-ethanolic extracts of three Cameroonian medicinal plants against Aedes albopictus. Asian Pacific Journal of Tropical Biomedicine, 6(11), 931–936. https://doi.org/https://doi.org/10.1016/j.apjtb.2016.09.004

Fujiwara, G. M., Annies, V., de Oliveira, C. F., Lara, R. A., Gabriel, M. M., Betim, F. C. M., … Zanin, S. M. W. (2017). Evaluation of larvicidal activity and ecotoxicity of linalool, methyl cinnamate and methyl cinnamate/linalool in combination against Aedes aegypti. Ecotoxicology and Environmental Safety, 139, 238–244. https://doi.org/https://doi.org/10.1016/j.ecoenv.2017.01.046

Govindarajan, M., & Benelli, G. (2016). Eco-friendly larvicides from Indian plants: Effectiveness of lavandulyl acetate and bicyclogermacrene on malaria, dengue and Japanese encephalitis mosquito vectors. Ecotoxicology and Environmental Safety, 133, 395–402. https://doi.org/https://doi.org/10.1016/j.ecoenv.2016.07.035

Grigoraki, L., Balabanidou, V., Meristoudis, C., Miridakis, A., Ranson, H., Swevers, L., & Vontas, J. (2016). Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus. Insect Biochemistry and Molecular Biology, 74, 61–67. https://doi.org/https://doi.org/10.1016/j.ibmb.2016.05.007

Haddi, K., Tomé, H. V. V., Du, Y., Valbon, W. R., Nomura, Y., Martins, G. F., … Oliveira, E. E. (2017). Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: A potential challenge for mosquito control. Scientific Reports, 7(October 2016), 1–9. https://doi.org/10.1038/srep46549

Ilham, R., Lelo, A., Harahap, U., Widyawati, T., & Siahaan, L. (2019). The Effectivity of Ethanolic Extract from Papaya Leaves (Carica papaya L.) as an Alternative Larvacide to Aedes spp. Open Access Macedonian Journal of Medical Sciences, 7(20), 3395–3399. https://doi.org/10.3889/oamjms.2019.432

Irwan, A., Mustikasari, K., & Ariyani, D. (2017). Pemeriksaan Pendahuluan Kimia Daun, Kulit Dan Buah Limau Kuit: Jeruk Lokal Kalimantan Selatan. Sains Dan Terapan Kimia, 11(2), 71–79.

Ishak, A. R., Dom, N. C., Hussain, H., & Sabri, N. H. (2014). Biolarvacidal Potential of Ipomoea Cairica Extracts Against Key Dengue Vectors. Procedia - Social and Behavioral Sciences, 153, 180–188. https://doi.org/https://doi.org/10.1016/j.sbspro.2014.10.052

Ishak, N. I. (2019). Efektivitas Ekstrak Kulit Buah Limau Kuit ( Citrus Amblycarpa ) sebagai Larvasida Aedes Aegypti Instar III Jurnal MKMI, 15(3), 302–310. https://journal.unhas.ac.id/index.php/mkmi/article/view/6533

Kasman, & Ishak, N. I. (2018). Analisis Penyebaran Penyakit Demam Berdarah Dengue di Kota Banjarmasin Tahun 2012-2016. Media Publikasi Promosi Kesehatan Indonesia, 1(2), 32–39. https://doi.org/10.31934/mppki.v1i2.176

Kasman, Ishak, N. I., Hastutiek, P., & Suprihati, E. (2019). GC-MS Analysis Citrus amblycarpa Peel Ethanol Exctract and Potential as a Bioinsecticides against Mosquitoes. In Kumpulan Abstrak Seminar Nasional 2019 “Percepatan Hilirisasi Hasil Penelitian di Era Industri 4.0” (p. 104). Retrieved from https://lppm.ipb.ac.id/publikasi/prosiding-seminar-ppm/?page_id=208&id=21&abstrak=777

Kasman, K., Ishak, N. I., Hastutiek, P., & Suprihati, E. (2021). Potential Extract Ethanol Citrus Amblycarpa as a Bioinsecticide Against Aedes Aegypti Larvae. Systematic Reviews in Pharmacy, 12(1), 1614–1618. https://repository.unair.ac.id/108978/

Kasman, K., Ishak, N. I., Hastutiek, P., Suprihati, E., & Mallongi, A. (2020). Identification of Active Compounds of Ethanol Extract of Citrus amblycarpa leaves by Analysis of Thin-layer Chromatography and Gas Chromatography-Mass Spectrometry as Bioinsecticide Candidates for Mosquitoes. Open Access Macedonian Journal of Medical Sciences, 8(T2), 1–6. https://doi.org/10.3889/oamjms.2020.5207

Maciel-de-Freitas, R., Avendanho, F. C., Santos, R., Sylvestre, G., Araújo, S. C., Lima, J. B. P., Valle, D. (2014). Undesirable consequences of insecticide resistance following Aedes aegypti control activities due to a dengue outbreak. PloS One, 9(3), 1–9. https://doi.org/10.1371/journal.pone.0092424

Maia, J. D., La Corte, R., Martinez, J., Ubbink, J., & Prata, A. S. (2019). Improved activity of thyme essential oil (Thymus vulgaris) against Aedes aegypti larvae using a biodegradable controlled release system. Industrial Crops and Products, 136, 110–120. https://doi.org/https://doi.org/10.1016/j.indcrop.2019.03.040

Mathalaimuthu, B., Shanmugam, D., Kovendan, K., Kadarkarai, M., Jayapal, G., & Benelli, G. (2017). Coleus aromaticus leaf extract fractions: A source of novel ovicides, larvicides and repellents against Anopheles, Aedes and Culex mosquito vectors? Process Safety and Environmental Protection, 106, 23–33. https://doi.org/10.1016/j.psep.2016.12.003

Maulana, S. (2021). Pengaruh Biolarvasida Daun Tanaman Sebagai Kontrol Vektor Nyamuk Aedes Aegypti Penyebab Demam Berdarah: A Literature Review. Jurnal Medika Hutama, 2(03), 978–989. http://jurnalmedikahutama.com/index.php/JMH/article/view/201

Mukandiwa, L., Eloff, J. N., & Naidoo, V. (2015). Larvicidal activity of leaf extracts and seselin from Clausena anisata (Rutaceae) against Aedes aegypti. South African Journal of Botany, 100, 169–173. https://doi.org/https://doi.org/10.1016/j.sajb.2015.05.016

Nerda, A. S. M. (2015). Analisis Kuantitatif Kadar Asam Askorbat Pada Limau Kuit (Citrus hystrix) Dengan Metode Spektrofotometri UV-VIS. Banjarmasin: Akademi Farmasi ISFI Banjarmasin.

Ocampo, C. B., Salazar-Terreros, M. J., Mina, N. J., McAllister, J., & Brogdon, W. (2011). Insecticide resistance status of Aedes aegypti in 10 localities in Colombia. Acta Tropica, 118(1), 37–44. https://doi.org/https://doi.org/10.1016/j.actatropica.2011.01.007

Pandiyan, G. N., Mathew, N., & Munusamy, S. (2019). Larvicidal activity of selected essential oil in synergized combinations against Aedes aegypti. Ecotoxicology and Environmental Safety, 174(March), 549–556. https://doi.org/10.1016/j.ecoenv.2019.03.019

Pavela, R., Benelli, G., Pavoni, L., Bonacucina, G., Cespi, M., Cianfaglione, K., … Maggi, F. (2019). Microemulsions for delivery of Apiaceae essential oils—Towards highly effective and eco-friendly mosquito larvicides? Industrial Crops and Products, 129, 631–640. https://doi.org/https://doi.org/10.1016/j.indcrop.2018.11.073

Pramadani, A. T., Hadi, U. K., & Satrija, F. (2020). Habitat Aedes aegypti dan Aedes albopictus sebagai Vektor Potensial Demam Berdarah Dengue di Kecamatan Ranomeeto Barat, Provinsi Sulawesi Tenggara. ASPIRATOR - Journal of Vector-Borne Disease Studies, 12(2), 123–136. https://doi.org/10.22435/asp.v12i2.3269

Purnama, S. G. (2017). Diktat Pengendalian Vektor. In Prodi IKM FK Universitas Udayana. Bali.

Ridha, M. R., & Nisa, K. (2010). Larva Aedes aegypti is Tolerant to Temepos in Banjarbaru. Vektora, III(2), 93–111. http://ejournal.litbang.kemkes.go.id/index.php/vk/article/view/3326

Sarma, R., Adhikari, K., Mahanta, S., & Khanikor, B. (2019). Insecticidal activities of Citrus aurantifolia essential oil against Aedes aegypti (Diptera: Culicidae). Toxicology Reports, 6, 1091–1096. https://doi.org/https://doi.org/10.1016/j.toxrep.2019.10.009

Sitepu, F. Y., Nasution, H., Supriyadi, T., & Depari, E. (2018). Epidemiological and Entomological Investigation of Dengue Fever Outbreak in South Nias District, North Sumatera Province, Indonesia, 2016. OSIR Journal, 11(3), 8-12. http://www.osirjournal.net/index.php/osir/article/view/123

Wang, W.-H., Urbina, A. N., Chang, M. R., Assavalapsakul, W., Lu, P.-L., Chen, Y.-H., & Wang, S.-F. (2020). Dengue hemorrhagic fever–A systemic literature review of current perspectives on pathogenesis, prevention and control. Journal of Microbiology, Immunology and Infection, 53(6), 963–978. https://doi.org/10.1016/j.jmii.2020.03.007

World Health Organization. (2012). Global Alert an Response (GAR) Dengue/Dengue Haemorrhagic Fever. Retrieved from http://www.who.int/csr/disease/dengue/en/

World Health Organisation. (2020). Dengue and severe dengue. Retrieved December 11, 2021, from WHO Fact Sheet website: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue

Yang, S., Bai, M., Yang, J., Yuan, Y., Zhang, Y., Qin, J., Sampietro, D. A. (2020). Chemical composition and larvicidal activity of essential oils from Peganum harmala, Nepeta cataria and Phellodendron amurense against Aedes aegypti (Diptera: Culicidae). Saudi Pharmaceutical Journal, 28(5), 560–564. https://doi.org/https://doi.org/10.1016/j.jsps.2020.03.007

Published
2022-02-24
How to Cite
Kasman, K., Ishak, N. I., Octaviana, E. S. L., & Ridha, M. R. (2022). Larvicidal Efficacy of Citrus amblycarpa Peel Extract Against the Larvae of Aedes albopictus. Diversity: Disease Preventive of Research Integrity, 2(2), 51-59. https://doi.org/10.24252/diversity.v2i2.26283
Abstract viewed = 371 times