BAKTERIOSIN ASAL BAKTERI ASAM LAKTAT SEBAGAI BIOPRESERVATIF PANGAN

  • Hafsan Hafsan
    (ID)

Abstract

Lactic Acid Bacteria origin Bacteriocins are protein substances that generally have small molecular weight as well as bactericidal activity that has the potential to be used as biopreservatif to prevent spoilage, and inhibit the growth of pathogenic bacteria in food. Bacteriocins as biopreservatif mechanism is to form holes in the membrane. Effect of cytoplasmic hole formation is the presence of bacteriocins impact that caused the membrane potential gradient changes and the release of intracellular molecules and influx of extracellular substance. The effect causes inhibited cell growth and cell death in the producing process that is sensitive to bacteriocins. Bacteriocins will work actively in the pH range 5-8, and are stable on heating to 80 ° C and the activity was marred by the presence of proteolytic enzymes. Advantages of the use of antimicrobial bacteriocins in mengeliminer ability of pathogenic microbes and food spoilage origin of milk and meat. Application as a therapeutic substance is possible due to its antimicrobial characteristics that are not toxic, capable of inhibiting the low levels and produced by bacteria belonging to GRAS (Generally recognised as safe) which microbes are not a health risk.

References

Abdelbasset M and Kirane Djamila., 2008. Antimicrobial activity of autochthonouslactic acid bacteria isolated from Algerian traditional fermented milk “Raïb” AfricanJournal of Biotechnology Vol. 7 (16), pp. 2908-2914, Available online at ttp://www.academicjournals.org/AJB ISSN 1684–5315 © 2008 AcademicJournals (diakses pada tanggal 12 April 2014).

Adetunji, 2007. Bakteriosin and Cellulose production by Lactic Acid Bacteria Isolated, African Journal of Biotechnology, Vol. 6 (22), pp. 2616-2619 Available online at http://www. African Journal of Biotechnology.org/ (diakses pada tanggal 12 April 2014).

Ali. G.R.R. and S. Radu. 1998. Isolation and Screening of Bakteriosin Producing LAB from Tempe. University of Malaysia.

Aymerich, T., Holo, H., Havarstein, L.S., Hugas, M., Garriga, M. and Nes, I.F. 1996. Biochemical and Genetic Characterization of Enterocin A from Enterococcus faecium, a New Anti listerial Bakteriosin in the Pediocin Family of Bakteriosins. App. and Environ. Microbiol. 62(5): 1676-1682.

Gonzalez, B., E. Glaasker, E.R.S. Kunji, A.J.M. Driessen, J.E. Suarez, and W.N. Konings. 1996. Bactericidal mode of action of plantaricin C. App.Environ. Microbiol., 62:2701-2709.

Heller, K.J. 2001. Probiotic Bacteria in Fermented Food: Product Characteristics and Starter Organism. Am. J. Clin. Nutr. 73, 374s-379s.

Luc De Vuyst. 2007. Bakteriosins from Lactic Acid Bacteria: Production, Purification, and Food Apllication, Journal of Molecular Micribiology and Biotechnology, 13, 194-1999).

Ouwehand, A.C., Vesterlund, S. 2004. Antimicrobial Components From Lactic Acid Bacteria. In Lactic Acid Bacteria: Microbiological and Functional Aspects, ed. Salminen, S.A., Von Wright, a., ouwehand, A.C. Marcel Dekker, new york: 375-395.

Salvadogo, A. 2006. Bacteriocins and Lactic Acid Bacteria-a miniriview. African Journal of Biotechnology 5(9):678-683.

Usmiati S, 2009. Penggunaan Bakteriosin sebagai Alternatif Pengawetan DagingAyam (Kasus Daging Ayam Berformalin), JITV 14(1) : 145-15411).

Van Belkum, M.J., J.Kok and G. Venema. 2002. Cloning, sequencing, and expression in Escherichia coli of IcnB, a third bacteriocin determinant from the Lactococcal bacteriocin plasmid p9B4-6. App.Environ.Microbiol. 58:572-577.

Yulneriwarni. 2006. Bakteri Asam Laktat sebagai Fermentatif, Biopreservatif dan Probiotik. Jurnal ilmu dan budaya volume: 27, no. 2.

Zoneyama, 2004. Bakteriosins Produced by Lactic Acid Bacteria and Their Usefor Food Preservation, Tohoku Journal of Agriculture Research, vol. 55, n0 1-2, pp.51-67.

Published
2014-07-12
Section
volume 8 nomor 2 tahun 2014
Abstract viewed = 1998 times