KAJIAN KARAKTER MORFOLOGI MIKROSPORA TEMBAKAU VIRGINIA YANG MENGALAMI CEKAMAN PELAPARAN DAN SUHU TINGGI SECARA IN VITRO

Baiq Farhatul Wahidah

doi:10.24252/teknosains.v8i2.1910

Baiq Farhatul Wahidah
(ID)

DOI: https://doi.org/10.24252/teknosains.v8i2.1910

Abstract

The effect of treatment combination by heat shock (34oC) and starvation medium on embryogenesis induction in microspore culture of selected genotype of Nicotiana tabacum L.cv. Virginia was examined.
The microspores which were isolated aseptically by maceration and centrifugation, were cultured on starvation medium (B medium) without sugar and nitrogen during 6 days at 34oC. The culture contained relatively homogeneous population of developed stage microspore (late uninucleate stage). The viability and development of microspore was obsevered. DAPI (4,6-diamidino-2-phenylindole) was used to stain the nucleus. Stained microspores were examined under a fluorescence microscope.
The result showed that during stress treatment by starvation and heat shock (34oC) the structure of microspore change into 3 types of embryogenic microspore. Type 1 was identical to the late uninucleate microspore stage; in type 2 the vacuole of microspore was fragmented and peripheral cytoplamic pocket contain the nucleus; and type 3 the nucleus was positioned in cytoplamic pocket in the centre of the microspore.

References

Bhojwani, SS and Bhatnaga, S.P. 1999 .The Embriology Of Angiosperm. New Delhi. Vicas Publishing Hause PVT LTD.

Cahyono, B. 1998. Tembakau Budidaya dan Analisis Usaha Tani. Yogyakarta. Penerbit Kanisius

Dunwell J.M. 1996. Microspore Culture. In Jain S.M; S.K. Sopory and R.E Veilleux (Eds). In Vitro Haploid Production in Higher Plants vol I Fundamental aspect in Method. Nederlands. Kluwer Academics Publishers. p:205-216

Ferrie, A.M.P. and Keller, W.A. 1995. Microspore Culture For Haploid Plant Production in : Gamborg O.L. and Philips. G.C. (eds) Plant Cell, tissue and Organ Culture Fundamental Methods. Heidelberg German. Springer verlag Berlin.

Indrianto,A; Barinova, I; Touraev,A and Heberle–Bors,E. 2001. Tracking Individual Wheat Microspores In vitro: Indentifikation of Embriogenic Microspores and Body Axis Formation in Embryo. Planta 212 : 163-174

Karp, G. 1999. Cell and Molecular Biology. Concepts and Experiments 2nd ed. Canada. Jhon Wiley and Sons.,Inc.. Pp.606-613

Kiviharju, E and Pehu,E. 1998. The Effect of Cold and Heat Pretreatment on Anther Culture Response of Avena sativa and Avena sterilis. Plant cell tissue and organ culture 54: 97-104

Kuswanto, 2005. Kerjasama kemitraan antara swasta dan Perguruan Tinggi dalam Penelitian untuk Meningkatkan daya Saing Daerah (Virginia lombok Espektasi dan Revitalisasi). PT Sadana Arif Nusa. Makalah disampaikan pada PENLOK Nasional METOPEN. 29-30 september 2005 (tidak dipublikasikan)

Li H.K; Javed, A.Q; and Kutty, K.K. 1988. The Influence of Different Temperature Treatments on Anthere Culture Response of Spring Wheat (Triticum aestivum L.) Plant sci. 57: 55-61

Ogawa, T.F; Hiroyuki and Ohkawa, Y. 1994. Introduction of Cell Division of Isolated Pollen Grains by Sugar Starvation in Rice. Breeding Science Vol. 44 : 75-77 No.1

Palmer C.E. and Keller, W.A. 1997. Pollen Embryos. In Sivana K.R. and V.K. Sawhney (eds) Pollen Biotecnology for Crop Production and Inprovement Cambridge University Press.

Raghavan, V. 1997. Molecular Embriology. Plant Molecular Biology. Cambridge University Press. New York.

Reddy VS; Leelavanti,S and Sen, S.K . 1985. Influence of Genotipe and Culture Medium in Microspore Callus Induction and Green Plant Regeneration in Anthera of Oryza sativa. Physiol. Plant 63: 309-314

Reynolds, T.L. and Raghavan, V. 1982. An Auto Radiographic Study of RNA Synthesis During Maturation and Germination of Pollen Grains of Hyosciamus niger. Protoplasma.

Suryowinoto, 1996. Kultur Jaringan Tumbuhan. Yogyakarta. Gadjah Mada University Press.