IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM L-ASPARAGINASE DARI ALGA COKLAT SARGASSUM sp

  • Mashuri Masri
    (ID)

Abstract

Molecular Identification of bacterial L-asparaginase activity from Sargassum sp were isolated from Barrang Lompo Island South Sulawesi in 2013. Among them three bacterial strains exhibiting glutaminase free L-asparaginase activity were identified as Pseudomonas putida, Enterobacter asburine dan Marinobacter arcticus on the basis of 16S rRNA gene sequencing. The Highest Max Score 1358 and Query Cover 98 % were Pseudomonas putida furthermore identified as bacterial L-asparaginase activity from Sargassum sp. Pseudomonas putida has Identities 756/765 (99%) with 778 bp Query length, Query cover 98% and Gaps 5/765 (0%).

References

Deval, A. G., Platas, G., Basilio, A., Cabello, A., Gorrochategui, J., Suay, I., Vicente, F., Portilllo, E., del Rio, M. J., Reina, G. G., and Pelaez, F., 2001, Screening of Antimicrobial Activities in Red, Green and Brown Macroalgae from Gran Canaria (Canary Islands, Spain). Int. Microbiologi, Vol. 4.

E-Moharam, M., Gamal-Eldeen, A.M., dan El-Sayed, S.T., 2010, Production, Immobilization and Anti Tumor Activity of L – Asparaginase of Bacillus sp R36, Journal of American Science, Vol. 6.

El-Bessoumy, A.A., Sarhan, M., dan Mansour, J., 2004, Production, Isolation, and Purification of L – Asparaginase from Pseudomonas Aeruginosa 50071 Using Solid state Fermentation, Journal of Biochemistry and Molecular Biology, Vol. 37.

Gashemi, Y., Ebrahimi, A., Rasoul-Amini, S., Zarrini, G., and Ghoshon, M. B., 2008, An Optimized Medium for Screening of L – Asparaginase Production by Eschericia coli, American Journal of Biochemistry an Biotechnology, Vol. 4.

Hadioetomo, R. S., 1993, Mikrobiologi Dasar dalam Praktek, PT. Gramedia Pustaka Utama, Jakarta.

Hay, M. E., 1996, Marine Chemical Ecology : What’s Known and What’s Next, J. Exp. Mar. Biol. Ecol., Vol. 200.

Kadi, A., 2005, Beberapa Catatan Kehadiran Marga Sargassum di Perairan Indonesia, Bidang Sumber daya Laut, Pusat Penelitian Oseanografi, LIPI, Jakarta, Vol. 30.

Kurnia, D. R. D., 2010, Studi Aktivitas Lipase dari Aspergillus niger sebagai Biokatalis pada Proses Gliserolisis untuk Menghasilkan Monoasilgliserol (Tesis), Universitas Diponegoro, Semarang

Lay, B. W., 1994, Analisis Mikroba di Laboratorium, Rajawali Pers, Jakarta.

Lee, Y. K., Lee, J. H., Lee, H. K., 2001, Microbial Symbiosis in Marine Sponges,J. Microbiol, Vol. 30.

Long, R. A., and Azam, F., 2001, Antagonistic Interactions among Marine Pelagic Bacteria, Appl Environ Microbiol, Vol. 67.

Natsir, H., Patong, A. R., Suhartono, M. T., and Ahmad A., 2010, Production and Characterization of Chitinase Enzyme from Sulili Hot Spring in South Sulawesi, Bacillus sp. HSA3-1a, Indo. J. Chem., Vol. 10.

Nofiani, R., 2008, Urgensi dan Mekanisme Biosintesis Metabolit Sekunder Mikroba Laut, Jurnal Natur Indonesia.

Perez-Matos, A. E., Rosado, W., Govind, N.S., 2007, Bacterial Diversity Associated with the Caribbean Tunicate Ecteinascidia turbinata, Department of Marine Sciences University of Puerto Rico, Vol. 92.

Poedjiadi, A., 1994, Dasar-dasar Biokimia, Universitas Indonesia, Jakarta.

Rasyid, A., 2001, Potensi Sargassum Asal Perairan Kepulauan Spermonde sebagai Bahan Baku Alginat, Kandidat Peneliti Puslitbang Oseanologi, LIPI, Jakarta.

Verma, N. K., Kumar, G., Kaur, and Ariand, S., 2007, L-Asparaginase : A Promosing Chemotherapeutic Agent, Critical Review Biotechnology, Vol. 27.

Zainuddin, E. N., dan Malina, A. C., 2009, Skrining Rumput Laut Asal Sulawesi Selatan sebagai Antibiotik melawan Bakteri Patogen pada Ikan, Laporan Penelitian Research Grant, Biaya IMHERE-DIKTI

Published
2014-07-12
Section
volume 8 nomor 2 tahun 2014
Abstract viewed = 1046 times