Gum Benzoin (Styrax benzoin) as Antibacterial against Staphylococcus aureus
Abstract
Gum benzoin (Styrax benzoin) is one of nontimber forest product classified into the resin group. The aim of the study was to determine the component from gum benzoin as an antibacterial against Staphylococcus aureus. Indonesian gum benzoin was obtained from North Sumatra and has antibacterial activity against S. aureus with the MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentrationo) values of its ethyl acetic extract of 1.00 mg/mL and 2.00 mg/mL, respectively. Thin layer chromatography (TLC) was used in purification of ethyl acetate extract of the gum benzoin. The active component was detected by TLC contact bioautography. The bands with Rf of 0.21, 0.77, and 0.87 had growth inhibition activity to the S. aureus. The active antibacterial band with Rf of 0.21 was isolated and it could be an alkaloid component.
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References
Balouiri, M., Sadiki, M., and Ibnsouda, S.K., 2016. Method for in vitro Evaluating Antimicrobial Activity. Journal of Pharmaceutiques Analysis 6, 71-79.
Batubara, I., Mitsunaga, T., and Ohadhi, H., 2009. Screening Antiacne Potency of Indonesian Medical Plants: Antibacterial, Lipase Inhibition, and Antioxidant Activities. Journal of Wood Science. 55, 230-235.
Castel, C., Fernandez, X., Lizzani, C.L., Loiseau, A.M., Perichet, C., Delbecque, C., and Arnaudo J.F., 2006. Volatile Constituents of Benzoin Gums (Siam and Sumatera). Part 2 study of Headspace Sampling Methods. Journal of Flavor and Fragrance. 21, 59– 67
Choma, I.M., and Edyta, M.G., 2011. Bioautography Detection in Thin Layer Chromatography. Journal of Chromatography 1218, 2684–2691
Delaveau, P., Lallouette, P., and Tessier, A.M., 1980. Drogues Vegetales Stimulant Activite Phagocytaire Systm Reticulo Endothelial. Journal of Planta Medicine. 40, 49-54.
Fessenden and Fessenden. 1982. Organic Chemistry. Third Edition. California, United State
Harbone, J.B., 1987. Phytocemical Methods. New York. United State
Hidayat, A., 2011. Fractionation of Flavonoid Group from Kepel Leaves (Stelechocarpus burahol) as an Antibacterial. Thesis. Bogor Agricultural University. Bogor
Hou, W.C., Lin, R.D., Cheng, K.T., Hung, Y.T., Cho, C.H., Chen, C.H., Hwang, S.Y., and Lee, M.H., 2003. Free Radicals Scavenging Activity of Taiwanese Native Plants. Journal of Phytomedicine. 10, 170-175
Jayusman. 1997. The Study of The Marketing System of Gum Benzoin (Styrax sp.): The Case Study in Simasom Vilage, Pahae Julu–North Tapanuli, North Sumatera. Forestry Magazine. The Forestry Research Center in Aek Nauli Pematang Siantar.
Jorgensen, J.H., and Ferraro, M.J., 2009. Antimicrobial Susceptibility Testing Are View of General Principles and Contemporary Practices. Journal of Medicine and Microbiology. 49, 1749–1755
Kim, Y.S., Oh, B.C., and Shin, D.H., 2004. The Extension of The Shelf Life of Cooked Rice by the Treatment with The Plant Extracts and Their Volatile Constituents. Journal of Food Science and Biotechnologhy. 13, 519-522
Kiswandono, A.A., Iswanto, A.H., Susilowati, A., and Lumbangtobing. 2016. The analysis of Cinnamid Acid and Phytochemical Screening of Bulu Gum Benzoin (Styrax benzoine var. Hiliferum) from North Tapanuli. Articles of National Seminar. 146-155
Markham, K.R., 1998. Techniques of Flavonoid Identification. Academic Pr. New York. United State
Pauletti, P.M., Araujo, A.R., Young, M.C.M., and Bolzani, V.S., 2006. The Styracaceae. The Journal of Pharmaceutical. 16, 576-590
Shin, S., 2003. Anti Aspergillus Activities of Plant Essential Oils and Their Combination Effects with Ketoconazole or Amphotericin B. Journal of Archieves Pharmazie Research. 263, 89-393
Teissedre, P.L., and Waterhouse, A.L., 2000. Inhibition of Oxidation of Human Low Density Lipoproteins by Phenolic Substances in Different Essential Oils Varieties. Journal of Agricultural and Food Chemistry. 48, 3801-3805
Yamaguchi, K., Suzuki, T., Katayama, A., Sasa M., and Iida, S., 1950. Insecticidal action of japanese plants. II. A general method of detecting effective fractions and its application to 24 species of insecticidal plants. Journal of Insect Control. 15, 62-70
Yulianty, R., Rante, H., Alam, G., and Tahrir, A., 2011. Screening and Analysis of TLC-Bioautography of Antimicrobial Compounds of Several Sponge Extracts from Marine Waters of Barrang Island Lompo South Sulawesi. Traditional Medicine Magazine. 16, 88-94
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