Active Compounds Of Ethyl Acetate Extract Of Stylotella Sp. Sponges From Selayar Islands Against Mcf-7 Breast Cancer Cells
Abstract
Stylotella sp. sponge is a type of marine invertebrate animals that are widely distributed in Indonesian marine areas. One of the spreading areas of the Stylotella sp. sponge is in the Selayar Islands, South Sulawesi, Indonesia. Sponges have potential as a drug because they contain secondary metabolites. The aim study was to identify the types of secondary metabolite compounds and determine the bioactivity of Stylotella sp. compounds against MCF-7 breast cancer cells. The extraction method was used in the process of obtaining isolates because it is easy and economical. Testing the level of purity of isolates was carried out with three eluent systems which showed a single stain result. FTIR characterization results showed that pure isolates contained alkaloids which were characterized by the appearance of O-H, C-N, and C-H groups. The activity of MCF-7 cells using the colorimetric method showed an IC50 value of 14987.50 µg / mL, not toxic.
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References
Asaf, Ruzkiah., Budimawan., Ahyar Ahmad. Variasi Aktivitas Kandungan Metabolit Sekunder Spons Berdasarkan Kondisi Habitat. Prosiding Indoaqua. Universitas Hasanuddin, 2012.
Cancer Chemoprevention Research Center. Protokol in vitro CCRC. Yogyakarta: Fakultas Farmasi UGM. (2008): h. 1-12.
Chumko Seok, et. al. Purification and Moleculer Docking Study of Angiotengin 1-Converting Enzyme (ACE) Inhibitory Peptides from Hydrolysates of Marine Sponge Stylotella Aurantium. Process Biochemistry, no. 8 (2016): h. 1-8.
Faizal, Muhammad Reza., M Kawaroe, F Satria. Potensi Senyawa Bioaktif Ekstrak Kasar Bakteri Simbion Spons sebagai Anthelmintika: Sebuah Uji Pendahuluan. Omni Akuatika 13, no .19 (2014): h. 77 – 84.
Goodlett, et.al. Alcohol Teratogenesis: Mechanisms of Damage and Strategies for Intervention. Experimental Biology and Medicine 12 no. 230 (2005): h. 394–406.
Handayani, Dian, et.al. Isolasi Senyawa Antimikroba dari Spon Laut Pseudoceratina purpurea Carter. Sains dan Teknologi Farmasi 15, no. 1 (2010): h. 58-66.
Handayani, Dian., M Yulia, Y Alen, N J de Voogd. Isolasi Senyawa Sitotoksik dari Spons Laut Petrosia sp. Jurnal JPB Perikanan 7, No. 1 (2012): 69–76.
Herdhiansyah, Ridho. Senyawa Lipid Spons Haliclona cymaeformis sebagai Biomarka dan Aktivitasnya terhadap Mikroba. Sains Dan Seni ITS 4, no.2, (2015): h. 2337-3520.
Ihsan, Fariz., I Setyawan, S Satrio, A D Jayanti, S Tito, E H Herningtyas. Ekstrak Purwaceng (Pimpinella Alpinna) sebagai Agen Kemopreventif dan Kemoterapi Kanker Paru (Kajian Antiproliferatif serta Uji Apoptosis melalui jalur p53,BCL-2,RB dan CASPASE-9). (2013).
Matsumoto, K., Y Yamada, M Takahashi, T Todoroki, K Mizoguchi, H Misaki, H Yuki. Fluorometric determination of carnitine in serum with immobilized carnitine dehydrogenase and diaphorase. Clin. Chem. no. 36 (1990): h. 2072–2076.
Murniasih, Tutik. Metabolit Sekunder dari Spons sebagai Bahan Obat-Obatan. Oseana 28, no. 3 (2003): h. 27-33.
Pettit, G R., Jayaram K. Srirangam, Delbert L. Herald, Karen L. Erickson, Dennis L. Doubek, Jean M. Schmidt, Larry P. Tackett, Gerald J. Bakus. Isolation and Structure of Stylostatin 1 from the Papua New Guinea marine Sponge Stylotella Aurantium. Organic Chemistry 57, no. 26 (1992): h. 7217-7220.
Ren, Wenying., Zhenhua Qiao, Hongwei Wang, Lei Zhu, Li Zhang. Flavonoids: Promising Anticancer Agents. Medicinal research Reviews 23, no. 4 (2003): h. 519-534.
Setyowati, Erna P., UA Jenie, BK Sudarsono, R Rahmat. Isolasi senyawa sitotoksik spons Kaliapsis. Majalah Farmasi Indonesia 18, No. 4 (2007): h. 183 – 189.
Subekti, Muhartono. Senyawa Brusein-A dari Buah Makassar (Brucea javanica (L.) Merr.) sebagai Antipriliferasi terhadap Sel Kanker Payudara T47D”. MKB 47, no. 1 (2015): h. 22-28.
Suparno. Kajian Bioaktif Spons Laut (Porifera: Demospongiae) Suatu Peluang Alternatif Pemanfaatan Ekosistem Karang Indonesia dalam Bidang Farmasi. Makalah Pribadi Falsafah Sains. Institut Pertanian Bogor, 2005.
Thurston, D.E. Chemistry and Pharmacology of Anticancer Drugs. Danvers: CRC Press, 2007.
Wewengkang, D S, Deiske A. Sumilat, Henki Rotinsulu. Karakterisasi dan Bioaktif Antibakteri Senyawa Spons Haliclona Sp. dari Teluk Manado. LPPM Bidang Sains dan Teknologi 1, no. 1 (2014): h. 71-85.
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