Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana) dan Uji Toksisitas Terhadap Artemia Salina Leach

  • Andi Nur Fitriani Abubakar Jurusan Kimia, Fakultas Sains dan Teknologi, UIN Alauddin Makassar
    (ID)
  • Aisyah Aisyah
  • Maswati Baharuddin

Abstract

Avocado seed (Persea americana) is recognized as one of medicinal plants. It contains several secondary metabolites, which have toxic activity. However, efforts to identify active compounds from avocado seeds (Persea americana) are still relatively rare. Therefore, isolation and toxicity assay have been conducted foward the active compound of avocado seed. Maceration one kilogram of seed dried powder by ethanol obtained 49,7464 gram extract. Separation of etanol extract by column chromatography generated 0,0698 grams of pure white needle crytal, which is positively triterpenoid based on Lieberman-Buchard test. In addition, infrared spectrum showed the existence of OH, C=C, C-C, C=O, -C-H, -CH3,-CH2 and C-O stretch, which support the presumed compound. The result of toxicity test on Artemia salina Leach showed that the extract, fraction and pure isolates of the etanol extract are toxic with LC50 values 13,274 g/mL; 9,528 g/mL and 8,128 g/mL, respectively.

Downloads

Download data is not yet available.

References

Ani, R., 2011, “Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n- Heksan dari Kulit Batang Api-api (Avicennia alba Blume).” Skripsi Sarjana, FMIPA Universitas Negeri Makassar.

Budiono, R., 1994, "Tinjauan Umum Tentang Famili Lauracea," Unitas, vol 2, no. 2.

Hariyana, A., 2008, Tumbuhan Obat dan Khasiatnya. Cet. VI; Jakarta: Penebar Swadaya.

Kholifah, 2009, “Dua Senyawa Terpenoid Alkohol Dari Rimpang Lengkuas Merah”, Jurnal Sains dan Terapan Kimia, Vol. 2 No. 1 (FMIPA.Universitas Lambung Mangkurat)., h. 46.

Mahmiah, 2006, ”Isolasi dan Identifikasi Senyawa Flavonoid dari Kulit Batang Tumbuhan Saccopetalum horsfieldii Benn Laboratory of Chemistry,” (Hang Tuah University, Surabaya).

Marlinda, M., 2013, "Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Biji Buah Alpukad (Persea americana Mill.).

Zuhrotun, 2007, "Aktivitas Antidiabetes Ekstrak Etanol Biji Buah Alpukat (Persea americana Mill.) Bentuk Bulat". Karya Ilmiah, Fakultas Farmasi, Universitas Padjadjaran, Jatinangor.

Published
2014-07-28
How to Cite
Abubakar, A. N. F., Aisyah, A., & Baharuddin, M. (2014). Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana) dan Uji Toksisitas Terhadap Artemia Salina Leach. Al-Kimia, 2(1), 25-32. https://doi.org/10.24252/al-kimia.v2i1.1635
Section
Article
Abstract viewed = 1741 times