Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana) dan Uji Toksisitas Terhadap Artemia Salina Leach
Abstract
Avocado seed (Persea americana) is recognized as one of medicinal plants. It contains several secondary metabolites, which have toxic activity. However, efforts to identify active compounds from avocado seeds (Persea americana) are still relatively rare. Therefore, isolation and toxicity assay have been conducted foward the active compound of avocado seed. Maceration one kilogram of seed dried powder by ethanol obtained 49,7464 gram extract. Separation of etanol extract by column chromatography generated 0,0698 grams of pure white needle crytal, which is positively triterpenoid based on Lieberman-Buchard test. In addition, infrared spectrum showed the existence of OH, C=C, C-C, C=O, -C-H, -CH3,-CH2 and C-O stretch, which support the presumed compound. The result of toxicity test on Artemia salina Leach showed that the extract, fraction and pure isolates of the etanol extract are toxic with LC50 values 13,274 g/mL; 9,528 g/mL and 8,128 g/mL, respectively.
Downloads
References
Ani, R., 2011, “Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n- Heksan dari Kulit Batang Api-api (Avicennia alba Blume).” Skripsi Sarjana, FMIPA Universitas Negeri Makassar.
Budiono, R., 1994, "Tinjauan Umum Tentang Famili Lauracea," Unitas, vol 2, no. 2.
Hariyana, A., 2008, Tumbuhan Obat dan Khasiatnya. Cet. VI; Jakarta: Penebar Swadaya.
Kholifah, 2009, “Dua Senyawa Terpenoid Alkohol Dari Rimpang Lengkuas Merah”, Jurnal Sains dan Terapan Kimia, Vol. 2 No. 1 (FMIPA.Universitas Lambung Mangkurat)., h. 46.
Mahmiah, 2006, ”Isolasi dan Identifikasi Senyawa Flavonoid dari Kulit Batang Tumbuhan Saccopetalum horsfieldii Benn Laboratory of Chemistry,” (Hang Tuah University, Surabaya).
Marlinda, M., 2013, "Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Biji Buah Alpukad (Persea americana Mill.).
Zuhrotun, 2007, "Aktivitas Antidiabetes Ekstrak Etanol Biji Buah Alpukat (Persea americana Mill.) Bentuk Bulat". Karya Ilmiah, Fakultas Farmasi, Universitas Padjadjaran, Jatinangor.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3)Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).