Molecular Docking of Physalis angulata and Schleichera oleosa as a Potential Inhibitor of Dengue methyltransferase

  • Rahmawaty Hasan Universitas Ibrahimy
    (ID)
  • Diana Lady Yunita Handoyo Universitas Ibrahimy
    (ID)
  • Laila Zukhruf Universitas Ibrahimy
    (ID)
Keywords: Physalis angulata, Schleichera oleosa, molecular docking, DHF

Abstract

Indonesia as the 2nd country with the largest Dengue Hemorrhagic Fever (DHF) cases among 30 countries with endemic areas. Oral infusion of Physalis angulata and Schleichera oleosa are potential plants that is traditionally applied for DHF treatment. This study aimed to analyze the chemical compounds of Physalis angulata and Schleichera oleosa as an alternative therapy to DHF utilizing the molecular docking procedure. The methods are macromolecule and ligand preparation, validation as well as molecular docking, data analysis and visualization. The compounds were downloaded from the PubChem database and dengue methyltransferase protein was obtained from PDB (1L9K). Molecular docking has interacted with the Autodock and analyzed by Pymol, Discovery Studio Visualizer and Ligplot. The results revealed that the test ligands had lower binding energies than the SAH as a native ligand, specifically 14-Hydroxyixocarpanolide -10.69 kcal/mol as a potential compound from Physalis angulata and Schleicherastatin 5 -10.25 kcal/mol as a potential compound from Schleichera oleosa. With hydrogen bonds and hydrophobic pockets, all of the test ligands bind the NS5 dengue methyltransferase active site. The results suggested that Physalis angulata and Schleichera oleosa possess offering compounds for inhibiting NS5 dengue methyltransferase as a DHF treatment.

Downloads

Download data is not yet available.

References

Abdulbaqi, J. 2022 Effect of guggulsterone, a sterol identified in Commiphora gileadensis (Becham), on the dengue virus enzymes: Pharmacokinetics, molecular docking and molecular dynamics simulations studies. Journal of King Saud University - ScienceVolume 34, Issue 6: 10.1016/j.jksus.2022.102140.

Atilgan, E., Hu, J. 2011. Improving Protein Docking Using Sustainable Genetic Algorithms. Journal of Computer Information Systems and Industrial Management Applications, 3: 248-255.

Bradacs G., Heilmann J., Weckerle C.S. Medicinal plant use in Vanuatu: A comparative ethnobotanical study of three islands. J. Ethnopharmacol. 2011;137:434–448.

Funkhouser, T. 2017. Lecture: Protein-ligand docking methods. Princeton University.

Hasan, R., 2021. Studi Bioinformatiika Kandungan Kimia Daun Kelor (Moringa oleifera) Terhadap Target Aksi Pemenuhan Zat Gizi Ibu Hamil. Jurnal Ilmiah Pannmed, 17 (2): 337-343.

Johansson, A. J. Brooks, D. A. Jans, and S. G. Vasudevan, J. 2001. A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-β and the viral helicase, NS3. Gen. Virol. 82: 10.1099/0022-1317-82-4-735.

Kirstein and Guido. 2008. Concept of Pharmacophores and Their Application in Computer-Aided Drug Design. ICS UNIDO Workshop. Italy: Trieste.

Laskowski R A, Swindells M B. 2011. LigPlot+: multiple ligand-protein interaction diagrams for drug discovery. J. Chem. Inf. Model., 51, 2778-2786.

Lim S.P. 2008. A scintillation proximity assay for dengue virus NS5 2’-O-methyltransferase-kinetic and inhibition analyses. Antiviral Res 2008, 80(3):360-369.

Lim S.P, Noble C.G, Shi P. 2015. The dengue virus NS5 protein as a target for drug discovery. Antiviral Res. 2015 Jul;119:57–67.

Lim, J.H.K. Koh, C.C. Seeh, C.W. Liew, A.D. Davidson. 2013. A crystal structure of the dengue virus non-structural protein 5 (NS5) polymerase delineates interdomain amino acid residues that enhance its thermostability and de novo initiation activities. Journal of Biological Chemistry, vol. 288, no. 43, pp. 31105–31114.

Lipinski, C. A., Lombardo, F., Dominy, B. W., & Feeney, F. J. 2001. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews, Vol. 23: 3-25.

Martinez, F.D., and Franco, J.L. 2018. Molecular Docking: Current Advances and Challenges. Revista Especializada en Ciencias Químico-Biológicas. 21 (1): 65-87.

Mervin L. H., Afzal A. M., Drakakis G., Lewis R., Engkvist O., Bender A. 2015. Target prediction utilising negative bioactivity data covering large chemical space. J. Cheminform. 7: 1–16. 10.1186/s13321-015-0098.

Ministry of Health of Indonesia. 2022. Health Profile of Indonesia Year 2021. Jakarta: Ministry of Health Republic Indonesia.

Panigrahi S.K., Desiraju G.R. 2007. Strong and Weak Hydrogen Bonds in the Protein-ligand Interface. School of Chemistry, University of Hyderabad, Hyderabad 500 046, India.

Podvinec, M., Scarsi, M., Schmidt, T. 2010. Discovered novel inhibitors of Dengue Methyltransferase through in vitro-driven virtual screening on a computer desktop grid. J Med Chem: 25;53(4):1483-95: 10.1021/jm900776m.

Rachmania, R.A. 2010. Thesis: Modification of Oseltamivir as an inhibitor of neuraminidase of Influenza A subtype H1N1 through docking and simulation of molecular dynamics. Depok: Department of Chemistry-FMIPA UI.

Saleh, M., and Kamisah Y. 2020. Potential medicinal plants for the treatment of Dengue Fever and Severe Acute Respiratory Syndrome-Coronavirus. Biomolecules, 11(42), 1-25.

S. Rajapakse, C. Rodrigo, and A. Rajapakse. 2012. Treatment of Dengue Fever: Infection and Drug Resistance, 5(1), 103-112.

Tambunan, U., Nasution, M., Azhima, F., Kerami, D. 2017. Modification of S-Adenosyl-l-Homocysteine as Inhibitor of Nonstructural Protein 5 Methyltransferase Dengue Virus Through Molecular Docking and Molecular Dynamics Simulation Drug Target Insights, 11(1): 1-14.

Thisyakorn, U. 2014. Latest developments and future directions in dengue vaccine. Ther Adv Vaccines; 2(1): 3–9.

Wallace AC, Laskowski RA, Thornton JM. 1995. Ligplot+: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng. 8, 127-134.

Wei D. Q., Zhang R., Du Q. S., Gao W. N., Li Y., Gao H., Wang S. Q., Zhang X., Li A. X., Sirois S. and Chou K. C. 2006. Anti-SARS drug screening by molecular docking. Amino Acids, 31(1), 73-80.

World Health Organization. 2016. WHO Traditional Medicine Strategy. Hong Kong: World Health Organization.

Zhao, T. S. Soh, J. Zheng, K. W. K. Chan, W. W. Phoo. 2015. A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication. PLoS Pathogens, 11(3), 1-27.

Published
2023-12-27
How to Cite
Hasan, R., Lady Yunita Handoyo, D., & Zukhruf, L. (2023). Molecular Docking of Physalis angulata and Schleichera oleosa as a Potential Inhibitor of Dengue methyltransferase. Al-Kimia, 11(2). https://doi.org/10.24252/al-kimia.v11i2.40433
Section
Article
Abstract viewed = 327 times