The Antibacterial Activity Testing on Acetyleugenol Against Staphylococcus aereus

  • Rahmawati Salsa Dinurrosifa STIFAR Yayasan Pharmasi Semarang
    (ID)
  • Indah Sulistyarini STIFAR Yayasan Pharmasi Semarang
    (ID)
  • Athika Darumas P STIFAR Yayasan Farmasi Semarang
    (ID)
  • Erwin Indriyanti STIFAR Yayasan Farmasi Semarang
    (ID)

Abstract

The main contents of clove oil are phenolic compounds, namely eugenol, eugenol acetate and gallic acid, as well as flavonoids. One of the derivatives of the eugenol compound is acetyl eugenol which was developed by the esterification reaction between eugenol and acetic acid anhydride using the sonochemical method. Synthesis of acetyleugenol was sonicated for 30 minutes at a temperature of 70-80°C. The % yield of the synthesized compound is calculated and continued with testing which includes solubility, melting point, FTIR and GC-MS tests, which are then tested for antimicrobial activity. The research results show that eugenol and acetic acid anhydride compounds with a NaOH catalyst can be synthesized using ultrasonic waves and produce a % yield of 3.50%. The resulting synthesis can melt starting at a temperature of 30°C and can dissolve in ethanol, methanol, chloroform and ether but does not dissolve in distilled water. FTIR testing on the acetyl eugenol compound showed the presence of -OH phenolic groups, C=C alkenes, C=C aromatics, C-O esters, and C=O esters. Acetyleugenol at concentrations of 1.25%, 2.5%, 5%, and 10% was able to act antibacterial on Staphylococcus aureus cultures with a density of 1.0 X 108 CFU/mL. 

Downloads

Download data is not yet available.

References

A. Azizah, I. Suswati, and S. M. Agustin, “Efek Anti Mikroba Ekstrak Bunga Cengkeh (Syzygium Aromaticum) Terhadap Methicillin-Resistant Staphylococcus Aureus (Mrsa)Secara in Vitro.,” Saintika Med., vol. 13, no. 1, p. 31, 2018, doi: 10.22219/sm.v13i1.5444.
C. Duri Kartika Et Al., “Pengaruh Gugus Metoksi Pada Senyawa 4- Metoksibenzaldehida Terhadap Sintesis Senyawa N’- (4-Metoksibenzilidene)-4-Hidroksibenzohidrazida Dengan Metode Iradiasi Gelombang Mikro,” 經濟研究, vol. 16, no. 1994, pp. 1–37, 2015, [Online]. Available: http://eprints.ums.ac.id/37501/6/BAB II.pdf.
Dan W. S. R. Rosanti Suryani Tince Mbatu, 2I Putu Bayu Kenanda, I Gede Yeyen Suharta, “Aktivitas Minyak Atsiri Daun Cengkeh Sebagai Antijamur Terhadap Candida albicans,” J. Media Sains, vol. 2, no. 1, pp. 61–65, 2018, [Online]. Available: http://jurnal.undhirabali.ac.id/index.php/mp3/article/view/360/326.
Dinurrosifa, R.S dan Indriyanti, E. A Green Synthesis Of Acetyl Eugenol By Sonochemical Method and Potential As Anti Inflammatory In-Vitro. Jurnal Kimia dan Pendidikan Kimia (JKPK), 7(3): 324-332.

H. Carrasco A. et al., “Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I),” J. Braz. Chem. Soc., vol. 19, no. 3, pp. 543–548, 2008, doi: 10.1590/S0103-50532008000300024.
J. B. Buil, J. Zoll, P. E. Verweij, W. J. G. Melchers, and A. M. C. Bergmans, Mycology. 2017.
J. Towaha, “Manfaat Eugenol Cengkeh dalam Berbagai Industri Di Indonesia,” Perspektif, vol. 11, no. 2, pp. 79–90, 2012.
L. E. García Reyes, “Kimia Organik,” J. Chem. Inf. Model., vol. 53, no. 9, pp. 1689–1699, 2013.
P. D. Panuluh, “Potensi Cengkeh (Syzigium Aromaticum) sebagai Antibakteri Methicillin Resistant Staphylococcus Aureus (MRSA),” J. Ilm. Kesehat. Sandi Husada, vol. 10, no. 2, pp. 270–274, 2019, doi: 10.35816/jiskh.v10i2.168.
R. Bulan, “Reaksi Asetilasi Eugenol dan Oksidasi Metil Iso Eugenol,” J. Eugenol, pp. 1–8, 2004.
S. Lahlou, L. F. L. Interaminense, P. J. C. Magalhães, J. H. Leal-Cardoso, and G. P. Duarte, “Cardiovascular Effects of Eugenol, A Phenolic Compound Present in Many Plant Essential Oils, in Normotensive Rats,” J. Cardiovasc. Pharmacol., vol. 43, no. 2, pp. 250–257, 2004, doi: 10.1097/00005344-200402000-00013.
T. Ernawati, A. Budiana, and T. Ernawati, “Bioaktivitas Turunan Metil Sinamat Terhadap Pertumbuhan Bakteri Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aureugenosa dan Jamur Candida albicans,” J. Kim. Val., vol. 1, no. 1, pp. 60–64, 2016, doi: 10.15408/jkv.v0i0.3154.
W. Widayat, B. Cahyono, and N. Ngadiwiyana, “Rancang Bangun Dan Uji Alat Proses Peningkatan Minyak Cengkeh Pada Klaster Minyak Atsiri Kabupaten Batang,” J. Ilmu Lingkung., vol. 10, no. 2, p. 64, 2012, doi: 10.14710/jil.10.2.64-69.
Y. D. Safitri and N. E. D. Purnamawati, “Perbandingan Aktivitas Antibakteri Ekstrak Methanol Gagang dan Bunga Cengkeh (Syzygium Aromaticum) terhadap Bakteri Staphylococcus aureus ATCC 25923,” J. Sains dan Kesehat., vol. 3, no. 3, pp. 410–416, 2021, doi: 10.25026/jsk.v3i3.354.
Yance Manopo, H. J. Sohilait, and H. Kainama, “Isolasi Eugenol Dari Bunga Cengkeh Dan Sintesis Eugenil Asetat (2-Methoxy-4-(2-Propen-1-YL)-Phenyl Acetate),” MJoCE, vol. 1, no. 1, pp. 54–63, 2011.
Published
2023-12-28
How to Cite
Dinurrosifa, R. S., Sulistyarini, I., Darumas P, A., & Indriyanti, E. (2023). The Antibacterial Activity Testing on Acetyleugenol Against Staphylococcus aereus. Al-Kimia, 11(2). https://doi.org/10.24252/al-kimia.v11i2.42347
Section
Article
Abstract viewed = 157 times