Positive Blood Culture Results Predominantly Unrevealed among Adults Patients with Sepsis

  • Valentine Hursepuny Departemen Mikrobiologi Klinik Fakultas Kedokteran Universitas Hasanuddin
    (ID)
  • Baedah Madjid Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Firdaus Hamid Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Mochammad Hatta Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Muh. Nasrum Massi Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Rizalinda Sjahril Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Yoeke Dewi Rasita Health Laboratory Center for Makassar
    (ID)
  • Lisa Tenriesa Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
  • Andi Rofian Sultan Department of Microbiology, Faculty of Medicine, Hasanuddin University
    (ID)
Keywords: Blood Culture, Sepsis, Infection

Abstract

Sepsis is increasing every year and can be life-threatening. In the diagnosis of sepsis, it is challenging to identify the causative agent of infection. This research aims to determine the proportion of positive blood culture in sepsis patients at Hasanuddin University Hospital, Makassar. Blood cultures were collected from 98 adult patients with sepsis manifestations. This research design is cross-sectional with a descriptive observation approach. The data analysed in descriptive statistics were utilised to summarise patient demographics, treatments, and outcomes. Thirteen of 98 samples were positive for microbial pathogens (13.26%). The elderly group had the highest percentage (76.5%); eighty-seven patients (88.77%) were treated with antibiotic therapy prior to blood culture collection. Cephalosporins are the most administered antibiotics (72.4%). Administration of empirical therapy prior to blood culture collection may decrease the sensitivity of culture results. The culture of the infectious focus should be done to assist in the consideration of antimicrobial treatment.

References

Cheng MP, Stenstrom R, Paquette K, Stabler SN, Akhter M, Davidson AC, et al. Blood Culture Results Before and After Antimicrobial Administration in Patients With Severe Manifestations of Sepsis: A Diagnostic Study. Annals of Internal Medicine. 2019;171(8):547–54.

Robert Gauer, Damon Forbes, Nathan Boyer. Sepsis: Diagnosis and Management. American Family Physician. 2020;101(7):409–18.

Sweeney TE, Liesenfeld O, May L. Diagnosis of bacterial sepsis: why are tests for bacteremia not sufficient? Expert Review of Molecular Diagnostics. 2019;19(11):959–62.

Tamma PD, Miller MA, Cosgrove SE. Recalibrating our approach to the management of sepsis how the four moments of antibiotic decision-making can help. Annals of America Thoracic Society. 2021;18(2):200–3.

Sanjaya BD, Djuang MH, Muniro FD, Chiuman L. Sepsis Risk Factors in Elderly Patients At Royal Prima Medan General Hospital. Jambura Journal of Health Science and Research. 2022;4(3):596–603.

Fathi M, Markazi-Moghaddam N, Ramezankhani A. A systematic review on risk factors associated with sepsis in patients admitted to intensive care units. Australian Critical Care. 2019;32(2):155–64.

World Health Organization.. Sepsis. [Internet] 2023. Available from : https://www.who.int/news-room/fact-sheets/detail/sepsis

Bassetti M, Giacobbe DR, Vena A, Wolff M. Diagnosis and Treatment of Candidemia in the Intensive Care Unit. Seminars in Respiratory and Critical Care Medicine. 2019;40(4):524–39.

Evans T. Diagnosis and management of sepsis. Clinical Medicine. 2018;18(2): 146-9.

Sinha M, Jupe J, Mack H, Coleman TP, Lawrence SM, Fraley I. Emerging Technologies for Rapid Diagnosis of Sepsis. Clinical Microbiology Reviews. 2018;31(2):1–26.

Centers for Diseases Control and Prevention. Blood Culture Contamination : An Overview for Infection Control and Antibiotic Stewardship Programs Working with the Clinical Laboratory. [Internet]. U.S Department of Health and Human Services. 2023. Available from : https://www.cdc.gov/antibiotic-use/core-elements/pdfs/fs-bloodculture-508.pdf

De Rose DU, Perri A, Auriti C, Gallini F, Maggio L, Fiori B, et al. Time to positivity of blood culture could inform decisions on antibiotics administration in neonatal early-onset sepsis. Antibiotics. 2021;10(2):1–7.

Li Y, Guo J, Yang H, Li H, Shen Y, Zhang D. Comparison of culture-negative and culture-positive sepsis or septic shock: a systematic review and meta-analysis. Critical Care. 2021;25(1):1–9.

Scheer CS, Fuchs C, Gründling M, Vollmer M, Bast J, Bohnert JA, et al. Impact of antibiotic administration on blood culture positivity at the beginning of sepsis: a prospective clinical cohort study. Clinical Microbiology and Infection. 2019;25(3):326–31.

Ter SK, Rattanavong S, Roberts T, Sengduangphachanh A, Sihalath S, Panapruksachat S, et al. Molecular detection of pathogens in negative blood culture in the Lao People’s Democratic Republic. The American Journal of Tropical Medicine and Hygiene. 2021;104(4):1582–5.

Kabi, A., Mohanty, A., Kumar, S. K., Singh, V., Jha, M. K., and Gupta. Clinical spectrum and risk factors for hospital-acquired septicemia in a tertiary care centre of North-East India. Journal of Family Medicine and Primary Care. 2020;9(8): 3949-54.

Martínez ML, Plata-Menchaca EP, Ruiz-Rodríguez JC, Ferrer R. An approach to antibiotic treatment in patients with sepsis. Journal of Thoracic Disease. 2020;12(3):1007–21.

Thorndike J, Kollef MH. Culture-negative sepsis. Current Opinion in Critical Care. 2020;26(5):473–7.

Hirosawa T, Sakamoto T, Hanai S, Harada Y, Shimizu T. Effect of Prior Antibiotic Treatment on Blood Culture in an Outpatient Department of General Internal Medicine: A Retrospective Case–Control Analysis. International Journal of General Medicine. 2023;16(June):2709–17.

Yang L, Lin Y, Wang J, Song J, Wei B, Zhang X, et al. Comparison of clinical characteristics and outcomes between positive and negative blood culture septic patients: A retrospective cohort study. Infection and Drug Resistance. 2021;14(October):4191–205.

Strich JR, Heil EL, Masur H. Considerations for Empiric Antimicrobial Therapy in Sepsis and Septic Shock in an Era of Antimicrobial Resistance. The Journal of Infectious Diseases. 2021;222(Suppl 2):S119–31.

Published
2024-01-31
How to Cite
Hursepuny, V., Baedah Madjid, Firdaus Hamid, Mochammad Hatta, Muh. Nasrum Massi, Rizalinda Sjahril, Yoeke Dewi Rasita, Lisa Tenriesa, & Andi Rofian Sultan. (2024). Positive Blood Culture Results Predominantly Unrevealed among Adults Patients with Sepsis. Alami Journal (Alauddin Islamic Medical) Journal, 8(1), 17-23. https://doi.org/10.24252/alami.v8i1.41777
Section
Article
Abstract viewed = 87 times