Purifikasi Antigen Outer Membrane Protein (OMP) Dari Isolat Salmonella enterica serovar Typhi
Abstract
Penelitian ini bertujuan mendapatkan protein murni dari antigen OMP isolat Salmonella enterica serovar typhi (S. typhi) dengan metode fraksinasi amonium sulfat dan dialisis. Fraksinasi dilakukan dengan variasi konsentrasi ammonium sulfat 10–20%; 20-40%; 40–60%, 60–80%, dan 80–100%. Kemudian filtrat dari setiap konsentrasi fraksinasi tersebut dilanjutkan dengan metode dialisis menggunakan membran selofan, kemudian diukur kadar proteinnya dengan metode Lowry. Hasil penelitian menunjukkan bahwa Kadar protein OMP tertinggi diperoleh sebesar 1, 641 mg/ml yaitu pada konsentrasi 20-40%.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
All articles published Open Access will be immediately and permanently free for everyone to read and download. We are continuously working with our author communities to select the best choice of license options: Creative Commons Attribution (CC BY)
Authors and readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, even commercially, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Biogenesis: Jurnal Ilmiah Biologi as publisher of the journal.