Sintesis Heptapeptida Linear (H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Oh) dengan Menggunakan Dic/Oksima sebagai Reagen Pengkopling
Abstract
A tetrapeptide, an analog of trypsin-modulating oostatic factor (TMOF), has been synthesised by using a method of solid-phase peptide synthesis on 2-chlorotrityl chloride resin. Fmoc strategy was applied on the synthesis. The formation of peptide bond was facilitated by DIC/oxime as coupling reagent. After all of amino acids were attached on the resin, tetrapeptidyl resin was added by a mixture of TFA:water:EDT(90:5:5) in dichloromethane to cleave the peptide.Crude peptide was purified by reverse-phase column chromatography and the purified peptide was analysed by TOF ES-MS spectroscopy.Downloads
References
Sudarwohadi, S. (1975). Pengaruh waktu tanam kubis dan dinamika populasi Plutella maculipennis Curt. dan Crocidolomia binotalis, Holtikultura, 3(4), p. 3-14.
Rueda, A. and Shelton, A.M. (2008). Diamonback Moth (DBM). Global Crop Pests. Cornell International Institute For Food, Agriculture, and Development.
Girsang, W. (2009). Dampak Negatif Penggunaan Pestisida. Fakultas Pertanian. Universitas Simalungun. Pematang Siantar.
Arnason, J.T., Mackinnon, S., Durst, A., Philogene, B.J.R., Hasbun, C., Sanchez, P., Poveda, L., San Roman, L., Isman, M.B., Satasook, C., Towers, G.H.N., Wiriyachitra, P., and
Mclaughlin, J.L., (1993). Insecticides in tropical plantswith non-neurotoxic modes of action. Phytochemical Potentialof Tropical Plants. p. 107-151.
Borovsky, D., Carlson, D.A., Griffin, P.R., Shabanowitz, J., and Hunt, D.F., (1993). Mass spectrometry and characterization of Aedes aegypty Trypsin Modulating Oostatic Factor (TMOF) and its analogs. Insec. Biochem. Molec. Biol., 23, p.703-712.
Nauen, R., Sorge, D., Sterner, A., and Borovsky, D. (2002). TMOF-like factor controls the biosynthesis of serine proteases in the larval gut of Heliothis virescens. Archives of Insect Biochemistry and Physiology. P.49-65.
Borovsky, D., (2003). Trypsin-modulating oostatic factor: a potential new larviside for mosquito control. Journal of Experimental Biology, 206, p.3869-3875.
Maharani, R., Yanti, E.F., Melati, D.I., and Sihotang, D. (2015). Synthesis of Trypsin-modulating Oostatic Factor (TMOF) and its Analogues by Solid-phase Peptide Synthesis Using DIC/Oxyma as Coupling Reagent. Procedia Chemistry, vol. 17, pp. 125-131
Chan, W. C. A. White., P.D. (2000). Fmoc solid phase peptide synthesis: A practical approach, Oxford University press, New York.
Copyright (c) 2018 Al-Kimia
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3)Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
