Identifikasi Komponen Senyawa Organik dan Uji Aktivitas Antiinflamasi dari Fraksi Etil Asetat Daun Cabai Rawit (Capsicum frutescens L.)
Abstract
Cayenne pepper was known to treat swelling of tissue cells and is thought to have anti-inflammatory activity. The aim study is to examine the anti-inflammatory activity of the ethyl acetate fraction of cayenne pepper and identify the components of organic compounds in the ethyl acetate fraction. The stages of this research include preparation, extraction, partitioning, phytochemical tests, anti-inflammatory activity tests using Human Red Blood Cells (HRBC), and identifying the components of organic compounds they contain. Among the three fractions obtained at the extraction stage, the ethyl acetate fraction had the best activity as an anti-inflammatory at a concentration of 1000 mg/L of 57.77%. The fractionation of the ethyl acetate fraction was carried out using Vaccum Liquid Chromatography (VLC), and four combined fractions were obtained. One of the combined fractions, code EA-4-5, with a weight of 0.188 grams, was analyzed using GC-MS and FTIR. The two main compounds obtained from the GC-MS analysis were dodecanoic acid (25.79%) and (-)-Loliolide (12.86%). The results of the FTIR interpretation of the EA-4-5 fraction showed absorption at a wave number of 3234.62 cm-1 (stretching vibration of the -OH functional group), 3088.03 cm-1 and 3028.24 cm-1 (stretching vibration of the functional group =CH), 2931.8 cm-1 and 2858.51 cm-1 (functional group strain vibration –CH), 1712.79 cm-1 (functional group strain vibration –C=O), and 1606.7 cm-1 (strain vibration functional group –C=C).
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References
Aswad S Hajratul. 2018. Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Cabai Rawit. (Capsicum frutescens L.) Terhadap Pertumbuhan Bakteri Penyebab Jerawat Propionibacterium acnes Secara In Vitro, Skripsi, Makassar. UIN Alauddin.
Bodeker G. 2000. Indigenous Medical Knowledge: The Law and Politics of Protection, Oxford: College.
Dorland. 2002. Kamus Kedokteran. Jakarta: EGC.
Guyton A. C. Hall J. E. 1997. Buku Ajar Fisiologi Kedokteran, Edisi 9, Jakarta: EGC.
Harborne J.B. 1987. Penuntun Cara Modern Menganalisis Tumbuhan, Bandung: ITB.
Katzung B.G. 2001. Farmakologi Dasar dan Klinik: Reseptor- reseptor Obat dan Farmakodinamik, Jakarta: EGC.
Kee J, dan Hayes E. R. 1993. Farmakologi Pendekatan Proses Keperawatan. Jakarta. EGC.
Leelaprakash G dan Mohan Dass S. 2010. In vitro antiinflammatory activity of methanol extract of Enicostemma axillare, International Journal of Drug Development & Research, 3(3), 189-196.
Muscari. 2005. Panduan Belajar Keperawatan Pediatrik Edisi 3, Jakarta: EGC.
Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, dan Sipeolu FO. 2010. Red Blood Cell Membran Stabilizing Potentials Of Extracts Of Lantana Camara and Its Fractions, International J. of Plant Physiology and Biochemistry, 2 (4): 46-51.
Purwaningsih Y dan Ersam T. 2007. Senyawa Santon Sebagai Antioksidan Dari Kayu Batang Garcinia tetranda Pierre, Akta Kimia Indonesia, Vol.2 (2), 103-108.
Puspa OK, Syahbanu I, dan Wibowo. MA. 2017. Uji Fitokimia dan Toksisitas Minyak Atsiri Daun Pala (Myristica fragrans Houtt) dari Pulau Lemukutan, Jurnal Kimia Khatulistiwa, 6(6), 6-16.
Sakat SS, Juvekar AR, dan Gambhire MN. 2010. In-Vitro Antioxidant and Antiinflammatory Activity of Methanol Extract of Oxalis corniculata Linn, International Journal of Pharmacy and Pharmaceutial Sciences, 2, 146-155.
Saleem TM, Azeem A, Dilip C, Sankar C, Prasanth N dan Duraisami R. 2011. Anti- inflammatory Activity of The Leaf Extracs of Gendarussa vulgaris Nees, Asian Pacific Journal of Tropical Biomedicine, 1(2), 147-149.
Setiadi. 2006. Cabai Rawit, Jenis dan Budidaya, Penebar Swadaya: Jakarta.
Shailesh G, Seema K dan Dwivedi S. 2010. In-vitro Anti-inflamatory Activity of Sarcostemma acidum Wight and Arn. Indian herb by Human Red Blood Cell Membrane Stabilization Method, International Journal of Pharmacy Teaching and Practice, 2, 184 188.
Sudirman dan Sabri. 2011. Aktivitas Antioksidan dan Komponen Bioaktif Kangkung Air (Inpomoea aquatic Forsk), Bogor: Institut Pertanian Bogor.
Susanti Febria Elvy. 2016. Pemurnian Senyawa Aktif Dari Daun Kecapi yang Berpotensi Sebagai Anti Kanker Secara Invitro Terhadap Sel Murine Leukimia, Skripsi, Bogor. IPB.
Svehla. 1990. Vogel Buku Teks Analisis Anorganik Kuantitatif Makro dan Semimikro, Jakarta, PT. Kalman Media Pustaka.
Zhang MW, BJ Guo, RF Zhang, JW Chi, ZC Wei, ZH Xu, Y Zhang dan XJ Tang. 2006. Sepration, Purification and Identification of Antioxidant Compositonsin Black Rice, Journal Agricultural Science in China, 5 (6), 431 – 440.
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