Preparasi Material Sensor Kreatinin dengan Metode Molecularly Imprinted Menggunakan Prekursor MBAA dan AMPSA

  • Karmanto Karmanto UNiversitas Islam Negeri Sunan Kalijaga
    (ID)
  • Ahmad Amjad Muzani Universitas Islam Negeri Sunan Kalijaga

Abstract

Synthesis and preparation of creatinine sensor material by molecularly imprinted method using N,N'-Methylenebisacrylamide (MBAA) and 2-Acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) as precursors, on nylon membrane had been conducted. The precursor i.e. MBAA and AMPSA is used as cross-linker monomer, and functional monomer respectively, with the creatinine as template.  The performance of creatinine sensor material been studied by instrument response (resistance) pattern analysis to concentration of creatinine.  The result of FTIR spectra and SEM photograph, indicated the formation of molecularly imprinted polymer (MIP) on nylon membrane. Logarithmic pattern is appears as instrument response to concentration change of creatinine. Linear correlation of instrument response and creatinine concentration is observed at concentration ranges 1 to 5 ppm of creatinine. Comparative studies show that performance of nylon coated membrane is better than non-coated membrane. Linearity, limit detection, and limit quantification value of coated membrane is 0.9592; 1.2066 ppm; and 4.0221 ppm respectively. On the other hand for non-coated membrane, linearity, limit detection, and limit quantification value obtainedis 0.8799; 2.1607 ppm and 7.2044 ppm respectively.

Downloads

Download data is not yet available.

References

Alexander C., Andersson H.S., Andersson L.I., Ansell R.J., Kirsch N., O’Mahony I.A.N., Whitcombe M.J.(2006). Molecular Imprinting Science and Technology: A Survey of the Literature for the Years Up to and Including 2003. J. Mol. Recognit, 19:106-180.

Baron, D.N. (1992).Patologi Klinik, diterjemahkan oleh Johannes Gunawan. Jakarta: Penerbit buku kedokteran EGC.

Choi, Y.S., Wang, K.H., Xu, M., Chung I.J. (2002). Synthesis of Exfoliated Polyacrylonitrile/Na-MMT Nanocomposites via Emulsion Polymerization. Chem. Mater, 14: 2936-2939.

Cormack, P.A.G. dan Elorza A.Z. (2004). Review Molecularly Imprinted Polymers: synthesis and characterization. J. Chromatogr, B 804 (2004) 173–182.

Doloksaribu, B. (2008). Pengaruh Proteksi Vitamin C terhadap Kadar Ureum, Kreatinin dan Gambaran Histopatologis Ginjal Mencit yang dipapar Plumbum. Medan: Universitas Sumatra Utara.

Farahmand, E., Ibrahim F., Hosseini S., Rothan H.A., Yusof R., Koole L.H., Djordjevic I. (2015).A Novel Approach for Application of Nylon Membranes in the Biosensing Domain.Applied Surface Science, 353: 1310–1319.

Fraden, Jacob, (2004). Handbook of Modern Sensors: Physics, Designs, and Application,3rdEdition. New York: Springer-Verlag, Inc.

Harmita, (2004). Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitunganya. Majalah Ilmu Kefarmasian, I(3) : 117 - 135.

Indonesian Renal Registry. (2015). 8th Report of Indonesian Renal Registry.Diakses dari www.indonesianrenalregistry.org.,pada 1 Januari 2018.

Khadro, B., Sanglar C., Bonhomme A., Errachid A., Jaffrezic-Renault N. (2010).Molecularly Imprinted Polymers (MIP) Based Electrochemical Sensor for Detection of Urea and Creatinine. Procedia Engineering, 5: 371–374.

Khan, G.F. dan Wernet W. (1997). A Highly Snsitive Amperometric Creatinine Sensor. Anal ChimActa, 351: 151-158.

Mattiasson, B. dan Lei Y. (2015). Molecularly Imprinted Polymers in Biotechnology. Switzerland: Springer International Publishing.

Pandey, R.P, Das A.K., Shahi V.K. (2015). 2 Acrylamido-2-methyl-1-propanesulfonic Acid Grafted Poly(vinylidene fluoride-co-hexafluoropropylene)-Based Acid-/ Oxidative-Resistant Cation Exchange for Membrane Electrolysis. ACS Appl. Mater. Interface, 7: 28524−28533.

Sergeyevaa, T.A., Gorbachb L.A., Piletska E.V., Piletskyc S.A., Brovkob O.O., Honcharovab L.A., Lutsykb O.D., Sergeevab L.M., Zinchenkoa O.A., El’skayaa A.V. (2013). Colorimetric Test-Systems for Creatinine Detection Based on Composite Molecularly Imprinted Polymer Membranes. Analytica Chimica Acta, 770: 161– 168.

Shon,Y-S., Wuelfing W.P., Murray R.W. (2001). Water-Soluble, Sulfonic Acid-Functionalized, Monolayer-Protected Nanoparticles and an Ionically Conductive Molten Salt Containing Them.Langmuir, 17: 1255-1261.

Wu, YH., dan Freeman B.D. (2009). Structure, Water Sorption, and Transport Properties of Crosslinked N-vinyl-2-pyrrolidone/ N,N-methylene bisacrylamide Films. Journal of Membrane Science, 344: 182–189.

Xue G. (1997). Fourier Transform Raman Spectroscopy and Its Application for the Analysis of Polymeric Materials. Prog.Polym. Sci., 22: 313-406.

Published
2018-12-24
How to Cite
Karmanto, K., & Muzani, A. A. (2018). Preparasi Material Sensor Kreatinin dengan Metode Molecularly Imprinted Menggunakan Prekursor MBAA dan AMPSA. Al-Kimia, 6(2), 97-112. https://doi.org/10.24252/al-kimia.v6i2.5592
Section
Article
Abstract viewed = 767 times