Kualitas Spermatozoa Ayam Bangkok dalam Pengencer Ringer Laktat dan Air Kelapa yang Disuplementasi Kuning Telur dan Glukosa
Quality of Bangkok Chicken Spermatozoa in Lactate Ringer and Coconut Water Extender Supplemented with Egg Yolk and Glucose
Abstract
Semen preservation can be done by freezing or cold storage (chilling). The purpose of this study was to determine the effect of the type of semen extender on the quality of Bangkok chicken spermatozoa, and to determine the best type of extender used for Bangkok chicken spermatozoa. This study used a completely randomized design (CRD) with six treatment levels (P1, P2, P3, P4, P5, P6) with four replications. The treatment level used was: P1 = Coconut Water, P2 = Coconut water + Egg Yolk, P3 = Coconut Water + Egg Yolk + Glucose, P4 = Ringer Lactate, P5 = Ringer Lactate + Egg Yolk, P6 = Ringer Lactate + Egg Yolk + Glucose. Parameters observed were motility, viability, and abnormality. The results showed that the type of extender treatment had a very significant effect (P<0.01) on spermatozoa motility, spermatozoa viability, and spermatozoa abnormalities. The conclusion of this study is that the effect of the type of semen extender on the quality of spermatozoa in Bangkok chickens is very significant, especially with the addition of egg yolk components. The best type of extender to use is coconut water extender with the addition of egg yolk.
Downloads
References
Azzam, A. H., Raharjo, D., Hernawati, T., Madyawati, S. P., & Utomo, B. (2022). Combination of 5% Dextrose Ringer’s solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature. Ovozoa: J Anim Reprod, 11, 109-14.
Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1), 19-24.
Bustani, G. S., & Baiee, F. H. (2021). Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders. Veterinary World, 14(5), 1220.
Feyisa, S.G., Park, Y.H., Kim, Y.M., Lee, B.R., Jung, K.M., Choi, S.B., Cho, C.Y. & Han, J.Y. (2018). Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds. Asian-Australas J. Anim Sci., 31(8), 1160-1168.
Fitriyah, F., Humaidah, N. & Suryanto, D. (2019). Pengaruh lama penyimpanan semen dalam pengencer Ringer’s Lactat yang disimpan pada suhu 4 oC terhadap kualitas spermatozoa ayam Magon. Jurnal Rekasatwa Peternakan, 1(1), 28-37.
Gibb, Z., & Aitken, R J. (2016). The Impact of Sperm Metabolism During In Vitro Storage: The Stallion As A Model, BioMed Research International, 9380609.
Hidayat, N., Hidayah, C.N., & Nugroho, A.P. (2021). Honey Supplementation in Lactate Ringer-Egg Yolk Extender on Quality of Pelung Chicken Spermatozoa Post-Chilling, Jurnal Kedokteran Hewan, 15(1), 7-10.
Jatra, M. L. D., Saleh, D. M., & Mugiyono, S. (2022). Pengaruh penambahan kuning telur dalam susu skim disimpan pada suhu 5 derajat celcius selama 1 jam terhadap motilitas dan fertilitas spermatozoa ayam Kampung. Jurnal Peternakan Sriwijaya, 11(2), 21-32.
Khaeruddin, K., & Amir, M. (2019). The effect of the combination of glucose concentration with the type of extenders on the quality of native rooster spermatozoa during storage. Chalaza Journal of Animal Husbandry, 4(2), 36-43.
Khaeruddin, A. N., Ardi, N., Fattah, A. H., & Armayanti, A. K. (2020a). Penentuan konsentrasi susu skim terbaik dalam pengencer semen ayam kampung berbahan dasar ringer laktat. Jurnal Veteriner, 21(2), 300-308.
Khaeruddin, K., Arifiantini, R. I., Sumantri, C., & Darwati, S. (2016). Kualitas spermatozoa ayam peranakan sentul dalam pengencer ringer laktat kuning telur dengan berbagai monosakarida (Quality of Sentul crossbreed chicken spermatozoa in ringer lactate-egg yolk extenders supplemented with various monosaccharide). Jurnal Kedokteran Hewan-Indonesian Journal of Veterinary Sciences, 10(2), 166-169.
Khaeruddin, K., Hidayat, A., & Syamsuryadi, B. (2019). Preservasi semen ayam menggunakan pengencer air kelapa hijau dengan berbagai tingkat kematangan. Agrominansia, 4(2), 109-119.
Khaeruddin, K., Junaedi, J., & Hastuti, H. (2020b). Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas Journal of Biological Diversity, 21(12).
Khaeruddin, K., & Kurniawan, M. E. (2020). Keberhasilan pembekuan semen ayam yang diencerkan dan diperkaya dengan glukosa, trehalosa, sukrosa dan laktosa. Jurnal Veteriner, 21(3), 476-484.
Khaeruddin, K., & Srimaharani, S. (2019). Use of old coconut water with various skim concentrations of milk as a extender for kampong chicken semen. Chalaza Journal of Animal Husbandry, 4(1), 24-29.
Khairuddin, K., Kurniawan, M. E., & Soman, S. (2019). Cryopreservation of Kampung rooster semen using egg yolk extender from four types of poultry with different concentrations. Jurnal Kedokteran Hewan-Indonesian Journal of Veterinary Sciences, 13(3).
Kusumawati, E. D., Woli, S. L., Krisnaningsih, A. T. N., Susanto, W. E., Rahadi, S., & Susilawati, T. (2018). Motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5 C menggunakan pengencer dan lama simpan yang berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis, 5(3), 102-105.
Lake, P. E., & Wishart, G. J. (1984). Comparative physiology of turkey and fowl semen. In Reproductive biology of Poultry (Vol. 25, pp. 151-160). British Poultry Science Ltd Edinburgh.
Manjunath, P. (2012). New insight into the understanding of the mechanism of sperm protection by extender components, Anim Reprod, 9(4), 809-815.
Misro, M. M., & Ramya, T. (2012). Fuel/energy sources of spermatozoa. Male Infertility: Contemporary Clinical Approaches, Andrology, ART & Antioxidants, 209-223.
Nataamijaya, A. G. (2010). Pengembangan potensi ayam lokal untuk menunjang peningkatan kesejahteraan petani. Jurnal Litbang Pertanian, 29(4), 131-138.
Nugroho, A.P. dan Saleh, D.M. (2016). Motilitas dan abnormalitas spermatozoa ayam Kampung dengan pengencer Ringer Laktat-putih telur dan lama simpan pada suhu 5 oC selama 48 Jam, Acta Veterinaria Indonesiana, 4(1), 35-41.
Parker, H.M., & McDaniel C.D. (2006). The immediate impact of semen extender and rate of dilution on the sperm quality index, ATP utilization, gas exchange, and ionic balance of broiler breeder sperm, Poult Sci, 85, 106-116.
Rahayu, S., Susilawati, T., & Soewondo, A. (2020). Biologi Reproduksi: Kajian Seluler dan Molekuler. Malang: UB Press.
Reddy, E.P., & Lakshmi, T.M. (2014). Coconut water-properties uses nutritional benefits in health and wealth and health and disease: A review. Journal of Current Trends in Clinical Medicine and Laboratory Biochemistry, 2(2), 6-18.
Rice-Evans C. (2004). Flavonoids and isoflavones: absorption, metabolism and bioactivity. Free Radic Biol Med., 36, 827-828.
Sangani, A.K, Masoudi, A.A., & Torshizi, R.V. (2017). Association of mitochondrial function and sperm progressivity in slow- and fast-growing roosters. Poult Sci., 96, 211-219.
Santiago-Moreno, J., Castaño, C., Toledano-Díaz, A., Coloma M.A., López-Sebastián, A., Prieto, M.T., & Campo, J.L. (2012). Cryoprotective and contraceptive properties of egg yolk as an additive in rooster sperm extenders. Cryobiology, 65(3), 230-234.
Santos, J.L.A., Vanderson, S., Bispo, Adriano, B.C., Filho, Isabella, F.D., Pinto, Lucas, S., Danta, Daiane, F., Vasconcelos, Fabíula, F., Abreu, Danilo, A.M., Isaac, A.M., Florêncio, P.F., Osmar, F.G., Marisa, H.G., Medeiros, & Humberto, R.M. (2013). Evaluation of chemical constituents and antioxidant activity of coconut water (Cocos nucifera L.) and caffeic acid in cell culture. Annals of the Brazilian Academy of Sciences, 85(4), 1235-1246.
Sarkar, P.K. (2020). Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15-27.
Setiawan, R., Priyadarshana, C., Tajima, A., Travis, A. J., & Asano, A. (2020). Localisation and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: relationship between ATP production pathways and flagellar motility. Reproduction, Fertility and Development, 32(7), 697–705.
Siudzińska, A., & Łukaszewicz, E. (2008). Effect of semen extenders and storage time on sperm morphology of four chicken breeds. Journal of Applied Poultry Research, 17(1), 101-108.
Suyadi, S. & Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Malang: Universitas Brawijaya Press.
Susilawati, T. (2011). Spermatology. Malang: UB Press.
Telnoni, S. P., Arifiantini, R. I., Yusuf, T. L., & Darwati, S. (2017). SK Kedu semen cryopreservation in Beltsville poultry semen extender and lactated ringer’s-egg yolk extender using dimethyl sulfoxide. Asian J. Poult. Sci, 11, 14-19.
Triadi, T., Ervandi, M., Fahrullah, F., Repi, T., & Indrianti, M. A. (2022). Kualitas semen ayam KUB menggunakan pengencer Ringer Dextrose dan Ringer Laktat pada suhu 5 Celcius. Jurnal Peternakan Sriwijaya, 11(1), 42-51.
Ulus, E., Kusumawati, E.D., dan Krisnaningsih, A.T.N. (2019). Pengaruh pengencer dan lama simpan semen ayam Kampung pada suhu ruang terhadap motilitas dan viabilitas spermatozoa. Jurnal Sains Peternakan, 7(1), 29-40.
