Motilitas dan Keutuhan Membran Plasma Spermatozoa Ayam Kampung yang Disimpan dengan Penurunan Suhu yang Berbeda
Motility and Plasma Membrane Integrity of Kampung Chicken Spermatozoa of Stored with Different Temperature Decreasing
Kata Kunci:
Ayam Kampung, Keutuhan Membran Plasma, Motilitas, Spermatozoa, Suhu
Abstrak
Penyimpanan semen pada suhu 50C berpengaruh terhadap kualitas spermatozoa. Tujuan penelitian ini adalah untuk mengetahui perbedaan variabel motilitas dan keutuhan membran plasma spermatozoa ayam Kampung yang disimpan langsung pada suhu 5 °C dengan penyimpanan dengan penurunan suhu yang bertahap setelah proses koleksi semen. Penelitian ini menggunakan rancangan acak lengkap dengan 3 taraf perlakuan dan 9 kali ulangan. Perlakuan yang digunakan yaitu penyimpanan pada suhu tetap 5 ⁰C selama 75 menit (P1), penyimpanan pada suhu yang diturunkan bertahap dari 41 ⁰C 30 menit - 21 ⁰C 30 menit - 5 ⁰C 15 menit (P2), dan penyimpanan pada suhu yang diturunkan bertahap dari suhu 41 ⁰C 15 menit - 31 ⁰C 15 menit - 21 ⁰C 15 menit - 11 ⁰C 15 menit - 5 ⁰C 15 menit (P3). Data hasil penelitian yang berdistribusi normal dianalisis menggunakan analisis ragam (ANOVA). Hasil penelitian menunjukkan bahwa perlakuan penurunan suhu yang berbeda tidak mempengaruhi (P>0.05) variabel motilitas dan keutuhan membran plasma spermatozoa selama penyimpanan. Rataan motilitas total, motilitas progresif dan keutuhan membran plasma yang didapatkan masing-masing 25.36-41.22%, 67.30-77.08% dan 92.35-96.29%. Kesimpulan penelitian ini adalah spermatozoa ayam Kampung yang dikoleksi dapat langsung disimpan pada suhu 5 °C tanpa adanya perlakuan penurunan suhu yang bertahap karena tidak ditemukan perbedaan variabel motilitas keutuhan membran plasma pada perlakuan tersebut.
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Referensi
Abbaspour, B., Sharifi, S. D., Ghazanfari, S., Mohammadi‐Sangcheshmeh, A., & Honarbakhsh, S. (2020). Effect of dietary supplementation of whole flaxseed on sperm traits and sperm fatty acid profile in aged broiler breeder roosters. Reproduction in Domestic Animals, 55(5), 594-603.
Andhare, V.V., & Poojary, B. (2015). Effect of temperature on membrane integrity of human spermatozoa. Research Journal of Recent Sciences, 4(ISC-2014), 152-154.
Aurich, C. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Animal Reproduction Science, 89(1-4), 65-75.
Blank, M. H., Ruivo, L. P., Novaes, G. A., Lemos, E. C., Losano, J. D., Siqueira, A. F., & Pereira, R. J. (2021). Assessing different liquid-storage temperatures for rooster spermatozoa. Animal Reproduction Science, 233, 106845.
Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1), 19-24.
Canvin, A. T., & Buhr, M. M. (1989). Effect of temperature on the fluidity of boar sperm membranes. Reproduction, 85(2), 533-540.
Chakraborty, S., & Saha, S. (2022). Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertility Society Journal, 27(1), 1-12.
Chanavat, E., Vidament, M., Defoin, L., Duchamp, G., Levillain, N., Yvon, J. M., Le Vern, Y., Kerboeuf, D. & Magistrini, M. (2005). Effect of storage and temperature on in vitro stallion sperm parameters and fertility rate. Animal Reproduction Science, 89(1), 318.
Clarke, R. N., Sexton, T. J., & Ottinger, M. A. (1982). Effects of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen. Poultry Science, 61(9), 1912-1917.
Colagar, A. H., Karimi, F., & Jorsaraei, S. G. A. (2013). Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men. Iranian Red Crescent Medical Journal, 15(9), 780.
Davila, S. G., Campo, J. L., Gil, M. G., Castano, C., & Santiago-Moreno, J. (2015). Effect of the presence of hens on roosters sperm variables. Poultry Science, 94(7), 1645-1649.
Dcunha, R., Hussein, R. S., Ananda, H., Kumari, S., Adiga, S. K., Kannan, N., Zhao, Y., & Kalthur, G. (2020). Current insights and latest updates in sperm motility and associated applications in assisted reproduction. Reproductive Sciences, 1-19.
Gaczarzewicz, D., Udala, J., Piasecka, M., Blaszczyk, B., & Stankiewicz, T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turkish Journal of Biology, 39(4), 582-594.
Gautier, C., & Aurich, C. (2022). “Fine feathers make fine birds”–The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Animal Reproduction Science, 246, 106884.
Gibb, Z., & Aitken, R J. (2016). The Impact of Sperm Metabolism During In Vitro Storage: The Stallion As A Model, BioMed Research International, 9380609.
Gong, Y., Guo, H., Zhang, Z., Zhou, H., Zhao, R., & He, B. (2017). Heat stress reduces sperm motility via activation of glycogen synthase kinase-3α and inhibition of mitochondrial protein import. Frontiers in physiology, 8, 718.
Khaeruddin, K., & Kurniawan, M. E. (2020). Keberhasilan pembekuan semen ayam yang diencerkan dan diperkaya dengan glukosa, trehalosa, sukrosa dan laktosa. Jurnal Veteriner, 21(3), 476-484.
Khaeruddin, K., & Srimaharani, S. (2019). Use of old coconut water with various skim concentrations of milk as a diluent for kampong chicken semen. Chalaza Journal of Animal Husbandry, 4(1), 24-29.
Kusuma, P. W., Bebas, W., & Budiasa, M. K. (2018). Motilitas dan daya hidup spermatozoa ayam Pelung dalam pengencer kuning telur fosfat yang disimpan pada suhu 29 oC. Indonesia Medicus Veterinus, 7(2), 115-122.
Masoudi, R., Sharafi, M., Pourazadi, L., Davachi, N. D., Asadzadeh, N., Esmaeilkhanian, S., & Dirandeh, E. (2020). Supplementation of chilling storage medium with glutathione protects rooster sperm quality. Cryobiology, 92, 260-262.
Mehdipour, M., Kia, H. D., Najafi, A., Dodaran, H. V., & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3), 297-303.
Mussa, N. J., Ratchamak, R., Ratsiri, T., Vongpralub, T., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2021). Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Tropical Animal Health and Production, 53, 1-9.
Najafi, A., Taheri, R. A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A. A., & Nourani, M. R. (2019). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry science, 98(1), 440-446.
Sarkar, P. K. (2020). Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15-27.
Santiago-Moreno, J., Castaño, C., Coloma, M. A., Gómez-Brunet, A., Toledano-Díaz, A., López-Sebastián, A., & Campo, J. L. (2009). Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry Science, 88(12), 2661-2669.
Suyadi, S. & Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Malang: Universitas Brawijaya Press.
Tapia, J. A., Macias‐Garcia, B., Miro‐Moran, A., Ortega‐Ferrusola, C., Salido, G. M., Pena, F. J., & Aparicio, I. M. (2012). The membrane of the mammalian spermatozoa: much more than an inert envelope. Reproduction in domestic animals, 47, 65-75.
Vašíček, J., & Chrenek, P. (2013). Effect of storage temperature on the motility characteristics of rooster spermatozoa. Journal of Microbiology, Biotechnology and Food Sciences, 2 (special issue 1), 1685-1691.
White, I. G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility and Development, 5(6), 639-658.
Zubair, M., Ahmad, M., & Jamil, H. (2015). Review on the screening of semen by hypo‐osmotic swelling test. Andrologia, 47(7), 744-750.
Andhare, V.V., & Poojary, B. (2015). Effect of temperature on membrane integrity of human spermatozoa. Research Journal of Recent Sciences, 4(ISC-2014), 152-154.
Aurich, C. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Animal Reproduction Science, 89(1-4), 65-75.
Blank, M. H., Ruivo, L. P., Novaes, G. A., Lemos, E. C., Losano, J. D., Siqueira, A. F., & Pereira, R. J. (2021). Assessing different liquid-storage temperatures for rooster spermatozoa. Animal Reproduction Science, 233, 106845.
Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1), 19-24.
Canvin, A. T., & Buhr, M. M. (1989). Effect of temperature on the fluidity of boar sperm membranes. Reproduction, 85(2), 533-540.
Chakraborty, S., & Saha, S. (2022). Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertility Society Journal, 27(1), 1-12.
Chanavat, E., Vidament, M., Defoin, L., Duchamp, G., Levillain, N., Yvon, J. M., Le Vern, Y., Kerboeuf, D. & Magistrini, M. (2005). Effect of storage and temperature on in vitro stallion sperm parameters and fertility rate. Animal Reproduction Science, 89(1), 318.
Clarke, R. N., Sexton, T. J., & Ottinger, M. A. (1982). Effects of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen. Poultry Science, 61(9), 1912-1917.
Colagar, A. H., Karimi, F., & Jorsaraei, S. G. A. (2013). Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men. Iranian Red Crescent Medical Journal, 15(9), 780.
Davila, S. G., Campo, J. L., Gil, M. G., Castano, C., & Santiago-Moreno, J. (2015). Effect of the presence of hens on roosters sperm variables. Poultry Science, 94(7), 1645-1649.
Dcunha, R., Hussein, R. S., Ananda, H., Kumari, S., Adiga, S. K., Kannan, N., Zhao, Y., & Kalthur, G. (2020). Current insights and latest updates in sperm motility and associated applications in assisted reproduction. Reproductive Sciences, 1-19.
Gaczarzewicz, D., Udala, J., Piasecka, M., Blaszczyk, B., & Stankiewicz, T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turkish Journal of Biology, 39(4), 582-594.
Gautier, C., & Aurich, C. (2022). “Fine feathers make fine birds”–The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Animal Reproduction Science, 246, 106884.
Gibb, Z., & Aitken, R J. (2016). The Impact of Sperm Metabolism During In Vitro Storage: The Stallion As A Model, BioMed Research International, 9380609.
Gong, Y., Guo, H., Zhang, Z., Zhou, H., Zhao, R., & He, B. (2017). Heat stress reduces sperm motility via activation of glycogen synthase kinase-3α and inhibition of mitochondrial protein import. Frontiers in physiology, 8, 718.
Khaeruddin, K., & Kurniawan, M. E. (2020). Keberhasilan pembekuan semen ayam yang diencerkan dan diperkaya dengan glukosa, trehalosa, sukrosa dan laktosa. Jurnal Veteriner, 21(3), 476-484.
Khaeruddin, K., & Srimaharani, S. (2019). Use of old coconut water with various skim concentrations of milk as a diluent for kampong chicken semen. Chalaza Journal of Animal Husbandry, 4(1), 24-29.
Kusuma, P. W., Bebas, W., & Budiasa, M. K. (2018). Motilitas dan daya hidup spermatozoa ayam Pelung dalam pengencer kuning telur fosfat yang disimpan pada suhu 29 oC. Indonesia Medicus Veterinus, 7(2), 115-122.
Masoudi, R., Sharafi, M., Pourazadi, L., Davachi, N. D., Asadzadeh, N., Esmaeilkhanian, S., & Dirandeh, E. (2020). Supplementation of chilling storage medium with glutathione protects rooster sperm quality. Cryobiology, 92, 260-262.
Mehdipour, M., Kia, H. D., Najafi, A., Dodaran, H. V., & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3), 297-303.
Mussa, N. J., Ratchamak, R., Ratsiri, T., Vongpralub, T., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2021). Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Tropical Animal Health and Production, 53, 1-9.
Najafi, A., Taheri, R. A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A. A., & Nourani, M. R. (2019). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry science, 98(1), 440-446.
Sarkar, P. K. (2020). Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15-27.
Santiago-Moreno, J., Castaño, C., Coloma, M. A., Gómez-Brunet, A., Toledano-Díaz, A., López-Sebastián, A., & Campo, J. L. (2009). Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry Science, 88(12), 2661-2669.
Suyadi, S. & Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Malang: Universitas Brawijaya Press.
Tapia, J. A., Macias‐Garcia, B., Miro‐Moran, A., Ortega‐Ferrusola, C., Salido, G. M., Pena, F. J., & Aparicio, I. M. (2012). The membrane of the mammalian spermatozoa: much more than an inert envelope. Reproduction in domestic animals, 47, 65-75.
Vašíček, J., & Chrenek, P. (2013). Effect of storage temperature on the motility characteristics of rooster spermatozoa. Journal of Microbiology, Biotechnology and Food Sciences, 2 (special issue 1), 1685-1691.
White, I. G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility and Development, 5(6), 639-658.
Zubair, M., Ahmad, M., & Jamil, H. (2015). Review on the screening of semen by hypo‐osmotic swelling test. Andrologia, 47(7), 744-750.
Diterbitkan
2023-08-27
##submission.howToCite##
Khaeruddin, K. (2023). Motilitas dan Keutuhan Membran Plasma Spermatozoa Ayam Kampung yang Disimpan dengan Penurunan Suhu yang Berbeda: Motility and Plasma Membrane Integrity of Kampung Chicken Spermatozoa of Stored with Different Temperature Decreasing. Anoa: Journal of Animal Husbandry, 2(2), 80-87. https://doi.org/10.24252/anoa.v2i2.39734
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