Motilitas dan Keutuhan Membran Plasma Spermatozoa Ayam Kampung yang Disimpan dengan Penurunan Suhu yang Berbeda
Motility and Plasma Membrane Integrity of Kampung Chicken Spermatozoa of Stored with Different Temperature Decreasing
Keywords:
Kampung Chicken, Plasme Membrane Integrity, Motility, Spermatozoa, Temperature
Abstract
Semen storage at a temperature of 50C affects the quality of spermatozoa. The purpose of this study was to determine the differences in motility and plasma membrane integrity of Kampung chicken spermatozoa variables that were stored directly at 5 °C with storage with a gradual decrease in temperature after the semen collection process. This study used a completely randomized design with 3 treatment levels and 9 replications. The treatment used was storage at a fixed temperature of 5 ⁰C for 75 minutes (P1), storage at a gradually lowered temperature from 41 ⁰C 30 minutes - 21 ⁰C 30 minutes - 5 ⁰C 15 minutes (P2), and storage at a gradually lowered temperature from temperature 41⁰C 15 minutes - 31 ⁰C 15 minutes - 21 ⁰C 15 minutes - 11 ⁰C 15 minutes - 5 ⁰C 15 minutes (P3). Research data that were normally distributed were analyzed using analysis of variance (ANOVA). The results showed that different temperature reduction treatments did not affect (P>0.05) the motility and plasma membrane integrity of spermatozoa variables during storage. The mean total motility, progressive motility and plasma membrane integrity were 25.36-41.22%, 67.30-77.08% and 92.35-96.29%, respectively. The conclusion of this study was that collected Kampung chicken spermatozoa could be directly stored at 5°C without any gradual reduction in temperature because no differences in plasma membrane integrity motility variables were found in the treatment.
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References
Abbaspour, B., Sharifi, S. D., Ghazanfari, S., Mohammadi‐Sangcheshmeh, A., & Honarbakhsh, S. (2020). Effect of dietary supplementation of whole flaxseed on sperm traits and sperm fatty acid profile in aged broiler breeder roosters. Reproduction in Domestic Animals, 55(5), 594-603.
Andhare, V.V., & Poojary, B. (2015). Effect of temperature on membrane integrity of human spermatozoa. Research Journal of Recent Sciences, 4(ISC-2014), 152-154.
Aurich, C. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Animal Reproduction Science, 89(1-4), 65-75.
Blank, M. H., Ruivo, L. P., Novaes, G. A., Lemos, E. C., Losano, J. D., Siqueira, A. F., & Pereira, R. J. (2021). Assessing different liquid-storage temperatures for rooster spermatozoa. Animal Reproduction Science, 233, 106845.
Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1), 19-24.
Canvin, A. T., & Buhr, M. M. (1989). Effect of temperature on the fluidity of boar sperm membranes. Reproduction, 85(2), 533-540.
Chakraborty, S., & Saha, S. (2022). Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertility Society Journal, 27(1), 1-12.
Chanavat, E., Vidament, M., Defoin, L., Duchamp, G., Levillain, N., Yvon, J. M., Le Vern, Y., Kerboeuf, D. & Magistrini, M. (2005). Effect of storage and temperature on in vitro stallion sperm parameters and fertility rate. Animal Reproduction Science, 89(1), 318.
Clarke, R. N., Sexton, T. J., & Ottinger, M. A. (1982). Effects of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen. Poultry Science, 61(9), 1912-1917.
Colagar, A. H., Karimi, F., & Jorsaraei, S. G. A. (2013). Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men. Iranian Red Crescent Medical Journal, 15(9), 780.
Davila, S. G., Campo, J. L., Gil, M. G., Castano, C., & Santiago-Moreno, J. (2015). Effect of the presence of hens on roosters sperm variables. Poultry Science, 94(7), 1645-1649.
Dcunha, R., Hussein, R. S., Ananda, H., Kumari, S., Adiga, S. K., Kannan, N., Zhao, Y., & Kalthur, G. (2020). Current insights and latest updates in sperm motility and associated applications in assisted reproduction. Reproductive Sciences, 1-19.
Gaczarzewicz, D., Udala, J., Piasecka, M., Blaszczyk, B., & Stankiewicz, T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turkish Journal of Biology, 39(4), 582-594.
Gautier, C., & Aurich, C. (2022). “Fine feathers make fine birds”–The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Animal Reproduction Science, 246, 106884.
Gibb, Z., & Aitken, R J. (2016). The Impact of Sperm Metabolism During In Vitro Storage: The Stallion As A Model, BioMed Research International, 9380609.
Gong, Y., Guo, H., Zhang, Z., Zhou, H., Zhao, R., & He, B. (2017). Heat stress reduces sperm motility via activation of glycogen synthase kinase-3α and inhibition of mitochondrial protein import. Frontiers in physiology, 8, 718.
Khaeruddin, K., & Kurniawan, M. E. (2020). Keberhasilan pembekuan semen ayam yang diencerkan dan diperkaya dengan glukosa, trehalosa, sukrosa dan laktosa. Jurnal Veteriner, 21(3), 476-484.
Khaeruddin, K., & Srimaharani, S. (2019). Use of old coconut water with various skim concentrations of milk as a diluent for kampong chicken semen. Chalaza Journal of Animal Husbandry, 4(1), 24-29.
Kusuma, P. W., Bebas, W., & Budiasa, M. K. (2018). Motilitas dan daya hidup spermatozoa ayam Pelung dalam pengencer kuning telur fosfat yang disimpan pada suhu 29 oC. Indonesia Medicus Veterinus, 7(2), 115-122.
Masoudi, R., Sharafi, M., Pourazadi, L., Davachi, N. D., Asadzadeh, N., Esmaeilkhanian, S., & Dirandeh, E. (2020). Supplementation of chilling storage medium with glutathione protects rooster sperm quality. Cryobiology, 92, 260-262.
Mehdipour, M., Kia, H. D., Najafi, A., Dodaran, H. V., & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3), 297-303.
Mussa, N. J., Ratchamak, R., Ratsiri, T., Vongpralub, T., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2021). Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Tropical Animal Health and Production, 53, 1-9.
Najafi, A., Taheri, R. A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A. A., & Nourani, M. R. (2019). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry science, 98(1), 440-446.
Sarkar, P. K. (2020). Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15-27.
Santiago-Moreno, J., Castaño, C., Coloma, M. A., Gómez-Brunet, A., Toledano-Díaz, A., López-Sebastián, A., & Campo, J. L. (2009). Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry Science, 88(12), 2661-2669.
Suyadi, S. & Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Malang: Universitas Brawijaya Press.
Tapia, J. A., Macias‐Garcia, B., Miro‐Moran, A., Ortega‐Ferrusola, C., Salido, G. M., Pena, F. J., & Aparicio, I. M. (2012). The membrane of the mammalian spermatozoa: much more than an inert envelope. Reproduction in domestic animals, 47, 65-75.
Vašíček, J., & Chrenek, P. (2013). Effect of storage temperature on the motility characteristics of rooster spermatozoa. Journal of Microbiology, Biotechnology and Food Sciences, 2 (special issue 1), 1685-1691.
White, I. G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility and Development, 5(6), 639-658.
Zubair, M., Ahmad, M., & Jamil, H. (2015). Review on the screening of semen by hypo‐osmotic swelling test. Andrologia, 47(7), 744-750.
Andhare, V.V., & Poojary, B. (2015). Effect of temperature on membrane integrity of human spermatozoa. Research Journal of Recent Sciences, 4(ISC-2014), 152-154.
Aurich, C. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Animal Reproduction Science, 89(1-4), 65-75.
Blank, M. H., Ruivo, L. P., Novaes, G. A., Lemos, E. C., Losano, J. D., Siqueira, A. F., & Pereira, R. J. (2021). Assessing different liquid-storage temperatures for rooster spermatozoa. Animal Reproduction Science, 233, 106845.
Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1), 19-24.
Canvin, A. T., & Buhr, M. M. (1989). Effect of temperature on the fluidity of boar sperm membranes. Reproduction, 85(2), 533-540.
Chakraborty, S., & Saha, S. (2022). Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertility Society Journal, 27(1), 1-12.
Chanavat, E., Vidament, M., Defoin, L., Duchamp, G., Levillain, N., Yvon, J. M., Le Vern, Y., Kerboeuf, D. & Magistrini, M. (2005). Effect of storage and temperature on in vitro stallion sperm parameters and fertility rate. Animal Reproduction Science, 89(1), 318.
Clarke, R. N., Sexton, T. J., & Ottinger, M. A. (1982). Effects of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen. Poultry Science, 61(9), 1912-1917.
Colagar, A. H., Karimi, F., & Jorsaraei, S. G. A. (2013). Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men. Iranian Red Crescent Medical Journal, 15(9), 780.
Davila, S. G., Campo, J. L., Gil, M. G., Castano, C., & Santiago-Moreno, J. (2015). Effect of the presence of hens on roosters sperm variables. Poultry Science, 94(7), 1645-1649.
Dcunha, R., Hussein, R. S., Ananda, H., Kumari, S., Adiga, S. K., Kannan, N., Zhao, Y., & Kalthur, G. (2020). Current insights and latest updates in sperm motility and associated applications in assisted reproduction. Reproductive Sciences, 1-19.
Gaczarzewicz, D., Udala, J., Piasecka, M., Blaszczyk, B., & Stankiewicz, T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turkish Journal of Biology, 39(4), 582-594.
Gautier, C., & Aurich, C. (2022). “Fine feathers make fine birds”–The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Animal Reproduction Science, 246, 106884.
Gibb, Z., & Aitken, R J. (2016). The Impact of Sperm Metabolism During In Vitro Storage: The Stallion As A Model, BioMed Research International, 9380609.
Gong, Y., Guo, H., Zhang, Z., Zhou, H., Zhao, R., & He, B. (2017). Heat stress reduces sperm motility via activation of glycogen synthase kinase-3α and inhibition of mitochondrial protein import. Frontiers in physiology, 8, 718.
Khaeruddin, K., & Kurniawan, M. E. (2020). Keberhasilan pembekuan semen ayam yang diencerkan dan diperkaya dengan glukosa, trehalosa, sukrosa dan laktosa. Jurnal Veteriner, 21(3), 476-484.
Khaeruddin, K., & Srimaharani, S. (2019). Use of old coconut water with various skim concentrations of milk as a diluent for kampong chicken semen. Chalaza Journal of Animal Husbandry, 4(1), 24-29.
Kusuma, P. W., Bebas, W., & Budiasa, M. K. (2018). Motilitas dan daya hidup spermatozoa ayam Pelung dalam pengencer kuning telur fosfat yang disimpan pada suhu 29 oC. Indonesia Medicus Veterinus, 7(2), 115-122.
Masoudi, R., Sharafi, M., Pourazadi, L., Davachi, N. D., Asadzadeh, N., Esmaeilkhanian, S., & Dirandeh, E. (2020). Supplementation of chilling storage medium with glutathione protects rooster sperm quality. Cryobiology, 92, 260-262.
Mehdipour, M., Kia, H. D., Najafi, A., Dodaran, H. V., & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3), 297-303.
Mussa, N. J., Ratchamak, R., Ratsiri, T., Vongpralub, T., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2021). Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Tropical Animal Health and Production, 53, 1-9.
Najafi, A., Taheri, R. A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A. A., & Nourani, M. R. (2019). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry science, 98(1), 440-446.
Sarkar, P. K. (2020). Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15-27.
Santiago-Moreno, J., Castaño, C., Coloma, M. A., Gómez-Brunet, A., Toledano-Díaz, A., López-Sebastián, A., & Campo, J. L. (2009). Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry Science, 88(12), 2661-2669.
Suyadi, S. & Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Malang: Universitas Brawijaya Press.
Tapia, J. A., Macias‐Garcia, B., Miro‐Moran, A., Ortega‐Ferrusola, C., Salido, G. M., Pena, F. J., & Aparicio, I. M. (2012). The membrane of the mammalian spermatozoa: much more than an inert envelope. Reproduction in domestic animals, 47, 65-75.
Vašíček, J., & Chrenek, P. (2013). Effect of storage temperature on the motility characteristics of rooster spermatozoa. Journal of Microbiology, Biotechnology and Food Sciences, 2 (special issue 1), 1685-1691.
White, I. G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility and Development, 5(6), 639-658.
Zubair, M., Ahmad, M., & Jamil, H. (2015). Review on the screening of semen by hypo‐osmotic swelling test. Andrologia, 47(7), 744-750.
Published
2023-08-27
How to Cite
Khaeruddin, K. (2023). Motilitas dan Keutuhan Membran Plasma Spermatozoa Ayam Kampung yang Disimpan dengan Penurunan Suhu yang Berbeda: Motility and Plasma Membrane Integrity of Kampung Chicken Spermatozoa of Stored with Different Temperature Decreasing. Anoa: Journal of Animal Husbandry, 2(2), 80-87. https://doi.org/10.24252/anoa.v2i2.39734
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