AKTIVITAS ANTIOKSIDAN Caulerpa lentillifera J.Agardh DENGAN METODE PEREDAMAN RADIKAL BEBAS 1,1-diphenyl-2 picrylhydrazil
Abstract
Green caviar (Caulerpa lentillifera J. Agardh) contains substances which potentially act as antioxidant. This research aim to determine the antioxidant activity of Green caviar extract using free radical scavenging method by 1,1-Diphenyl-2 Picrylhydrazil (Dpph). Green caviar was extracted with sequences extraction using n-hexane, ethyl acetate, and etanol 96% with the yield value for n-hexane, ethyl acetate, and ethanol are 2.256%, 4.176%, 5.548% respectively. Furthermore, the chemical contents of each extract were identified using phytochemical screening and TLC qualitative test with n-hexane : ethyl acetate ( 8 : 2 ) as mobile phase and silica gel 60 F254 as stationary phase. The results showed that each green caviar extract has antioxidant activity with IC50 value of n- hexane extract 61.057 μg/mL identified as moderate antioxidant (50-100 μg/mL) ethyl acetate extract 41.756 μg/mL included as strong antioxidant (10-50 μg/mL), and ethanol 96 % which is 52.116 μg/mL classified as moderate antioxidant (50-100 μg/mL). Ethyl acetate is the best solvent to be used to extracting the antioxidant compounds of Green caviar.Downloads
References
Halliwell B. Free radicals and antioxidants: updating a personal view. Nutr Rev [Internet]. 2012 May 1 [cited 2020 Sep 24];70(5):257–65. Available from: https://academic.oup.com/nutritionreviews/article-lookup/doi/10.1111/j.1753-4887.2012.00476.x
Jahanban Isfahlan A, Mahmoodzadeh A, Hassanzadeh A, Heidari R, Jamei R. Antioxidant and antiradical activities of phenolic extracts from Iranian almond (Prunus amygdalus L.) hulls and shells. Turk J Biol. 2010 May 31;34(2):165–73.
MA S, S C, B W, Deolu-Sobogun SS. Antioxidant and Free Radical Scavenging Activities of Essential Oils. Ethn Dis. 2010;20(1 Suppl 1):78–82.
SANTOSO J, YOSHIE Y, SUZUKI T. The distribution and profile of nutrients and catechins of some Indonesian seaweeds. Fish Sci [Internet]. 2002 [cited 2020 Sep 24];68(sup2):1647–8. Available from: https://www.jstage.jst.go.jp/article/fishsci1994/68/sup2/68_sup2_1647/_article
R. W, A S, Subintoro. Pengembangan senyawa bioaktif dari biota laut. Makalah pada Forum Bioteknologi Kelautan dan Perikanan, Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan dan Perikanan, Depertemen Kelautan dan Perikanan. 2004;
Mahmudah N. Karakteristik Kimiawi Rumput Laut Lokal (Caulerpa sp.) dan Potensinya Sebagai Sumber Antioksidan. Politeknik Negeri Lampung; 2014.
A K, E.N D, Agustini T.W. Kajian potensi aktivitas antioksidan rumput laut caulerpa racemosa dari pantai sundak kabupaten gunungkidul. In: Prosiding Seminar Nasional Bioteknologi Kelautan dan Perikanan Tahunan Ke-1. 2012.
Aryudhani N. Kandungan senyawa fenol rumput laut caulerpa racemosa dan aktivitas antioksidannya. Institut Pertanian Bogor; 2007.
Hanani E, Munim A, Sekarini R. IDENTIFIKASI SENYAWA ANTIOKSIDAN DALAM SPONS CALLYSPONGIA SP DARI KEPULAUAN SERIBU. Maj Ilmu Kefarmasian [Internet]. 2005 Dec 7 [cited 2020 Sep 24];2(3):127–33. Available from: http://psr.ui.ac.id/index.php/journal/article/view/3389
Pramesti R, Kelautan JI, Perikanan F, Kelautan I. Aktivitas Antioksidan Ekstrak Rumput Laut Caulerpa serrulata Dengan Metode DPPH (1,1 difenil 2 pikrilhidrazil). Akt Antioksidan Ekstrak Rumput Laut Caulerpa serrulata Dengan Metod DPPH (1,1 difenil 2 pikrilhidrazil) [Internet]. 2013 Apr 18 [cited 2020 Sep 24];2(2):7–15. Available from: http://ejournal.undip.ac.id/index.php/buloma
Tias FN. Aktivitas Antioksidan dan Komponen Bioaktif Dari Keong Pepaya (Melo sp.). Institut Pertanian Bogor; 2010.
K S, Yenrina R. Antioksidan Alami dan Sintetik. Padang: Andalas University Press; 2015. 77 p.
Ukieyanna E. Aktivitas Antioksidan, Kadar Fenolik, dan Flavonoid Total Tumbuhan Suruhan (Peperomia pellucida L. Kunth). Bogor; 2012.
Handayani V, Ahmad AR, Sudir M. Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH. Pharm Sci Res [Internet]. 2014 Aug 21 [cited 2020 Sep 24];1(2):86–93. Available from: http://psr.ui.ac.id/index.php/journal/article/view/3321
Pusat E, Bioteknologi -Lipi P. KANDUNGAN KUERSETIN DAN POLA PROTEOMIK VARIETAS JAMBU B ATU (Psidium guajava L.) TUMBUH LIAR DIKAWASAN CIBINONG, BOGOR [QUERCETIN CONTENT AND PROTEOMIC PROFILE OF GVXVA(Psidium guajava L.) VARIETIES WILD GROWING IN CIBINONG, BOGOR DISTRICT] [Internet]. Vol. 10, Berita Biologi. Desember; 2010 [cited 2020 Sep 24]. Available from: https://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/756
Copyright (c) 2020 Jurnal Kesehatan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
