AKTIVITAS PENGHAMBATAN FRAKSI DAUN DELIMA (Punica granatum L.) TERHADAP BAKTERI Mycobacterium tuberculosis

Afrisusnawati Rauf; Nurshalati Tahar; Resnia Resnia

doi:10.24252/kesehatan.v0i0.18044

Afrisusnawati Rauf
Nurshalati Tahar
Resnia Resnia

DOI: https://doi.org/10.24252/kesehatan.v0i0.18044

Abstract

Punica granatum L., commonly known as pomegranate, has emerged as a medicinal plant with potential antimicrobial activity. This study aims to identify this activity against the bacteria Mycobacterium tuberculosis. Pomegranate leaf extract is made using solvents (ethanol 96%), then partitioned using n-butanol solvents and fractionated using vacuum liquid chromatography method. After obtaining the fractional results, the inhibitory activity effect of the fraction was studied using the Mycobacterium tuberculosis H37RV strain and compared to commercial antibiotic Isoniazid using the MODS method. Pomegranate leaf fraction shows antibacterial activity against Mycobacterium tuberculosis at a concentration of 1000 ppm, as seen from the absence of cord at such concentrations. Therefore this plant can be an important source of new antimicrobial compounds to treat the bacteria Mycobacterium tuberculosis.

Downloads

Download data is not yet available.

References

AlMatar, Manaf. "Evaluation of Polyphenolic Profile and Antibacterial Activity of Pome- granate Juice in Combination with Rifampin (R) against MDR-TB Clinical Isolates." Current Pharmaceutical Biotechnology 20, no. 0 (2019).

Al-Zoreky, N. "Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels." International journal of food microbiology 134 (2009): 244-248.

Amalia, Alfi. "Aktivitas Antibakteri Ekstrak Etil Asetat Daun Sembung (Blumea balsamifera L.) Terhadap Pertumbuhan Bakteri Meticillin Resistant Staphylococcus Aureus." Prosiding Seminar Nasional Biotik 2017. 2017.

Brady. "The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis." J Vis Expo 11, no. 17 (2008).

Braga LC, Leite AAM, Xavier KGS, Takahashi JA, Bemquerer MP, Chartone- Souza E, et al. "Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus." Can J Microbiol 51, no. 7 (2005): 541.

Celik I, Temur A, ISIK I. "Hepatoprotective role and antioxidant cap- acity of pomegranate (Punica granatum) flowers infusion against trichloro- acetic acid-exposed in rats." Food Chem Toxicol. 47 (2009): 145–149.

Hemani. "EVALUATION OF ANTIMICROBIAL PROPERTY OF EXTRACT OF PUNICA GRANATUM (L.) ON ORAL PATHOGENS." Int. J. Life Sci. Pharma Res 8, no. 3 (2018): 35-40.

Howell, Ami. "The Pomegranate: Effects on Bacteria and Viruses That Influence Human Health." Evidence-Based Complementary and Alternative Medicine (Hindawi Publishing Corporation), 2013: 1-11.

Howell, Amy B. "The Pomegranate: Effects on Bacteria and Viruses That Influence Human Health." Evidence-Based Complementary and Alternative Medicine (Hindawi Publishing Corporation), 2013.

Melendez, P.A., and V.A Capriles. "Antibacterial properties of tropical plants from Puerto Rico." Phytomedicine 2006, 13 (2006): 272-276.

Miguel MG, Neves MA, Antunes MD. "Pomegranate (Punica granatum L.): A medicinal plant with myriad biological properties-A short review." J Medicinal Plants Res 4, no. 25 (December 2010): 2836.

Mitnick, Carole D. "Tuberculosis pharmacotherapy: strategies to optimize patient care." Expert Opin Pharmacother 10, no. 3 (2009): 381–401.

Prihantoro, Teguh. "Antibacterial Effect of Pomegranate's (Punica granatum) Rind Extract Againts Shigella disentriae in Vitro." Jurnal Kedokteran Brawijaya 22, no. 3 (2006).

Rajandeep Kaur, Harpreet Kaur. "Antitubercular Activityand Phytochemical Screening of Selected Medicinal Plant." Oriental Journal of Chemistry 31, no. 1 (2015): 597-600.

Rockwood, Neesha. "Assessment of treatment response in tuberculosis." Expert Rev Respir Med 10, no. 6 (2016): 643–654.

Russel, DG. "Graduation time." Nature, 2013: 502.

Segun. "Comparison of Efficiency of Different Solvents used for the Extraction of Phytochemicals from the." International Journal of Science and Research (IJSR) 4, no. 7 (2013): 835-838.

WHO. Global Tuberculosis Report 2013. 2013.

Yuan T, Ding YQ, Wan CP, Li LY, Xu JL, Liu K, Slitt A, Ferreira D, Khan IA, Seeram NP. "Antidiabetic ellagitannins from pomegranate flowers: inhibition of alpha-glucosidase and lipogenic gene expression." Org Lett 14 (2012): 5358–5361.