The Potential of α- glucosidase Inhibition from Endophytic Fungi Associated in Portulaca oleracea L

  • Siska umi
    (ID)
  • Asriani Suhaenah
    (ID)
  • Saripa Indira Syahraini Latuconsina
    (ID)
DOI: https://doi.org/10.24252/djps.v6i1.38049
Keywords: Portulaca oleracea L, fungi endophytes, antidiabetes, inhibition, α -glucosidase

Abstract

Introduction: The discovery of endophytic microbes, which are microorganisms that reside within plant tissues and can produce bioactive compounds with similar properties to their host plants, is one possible solution to this issue. Endophytic fungi are capable of manufacturing antibacterial, antifungal, antiviral, anticancer, antimalarial, and antidiabetic agents. Portulaca oleracea L. is a weed that has spread globally. Portulaca oleracea L.has been utilized as a nutritious and medicinal plant for countless centuries. Aims: This study seeks to assess the antidiabetic potential of the endophytic fungi Portulaca oleracea L. Methods: In this study, isolation, macroscopic testing, fermentation, and antidiabetic activity testing were conducted using the alpha-glucosidase method. Twelve isolates with macroscopically distinct characteristics were obtained from of isolating the endophytic fungi. The procedure then advances to the fermentation and ethyl acetate extraction phases. Result: The results showed that there was inhibition of the α-glucosidase enzyme in hydrolyzing substrates into glucose by secondary metabolite extracts of fungi associated with the plant Portulaca oleracea L. with the highest percentage inhibition on the lab scale in the 12th isolate (83.92%). Conclusion: This measurement gives good results as an α-glucosidase inhibitor, so its potential as a source of antidiabetic drug substances is very high.

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References

Agyare C., Eunice B., John A., Yan D. & Louis A., 2015, Anti-infective and Anti-inflammatory Properties of Portulaca oleracea L, Journal of Medicinal Plant Research, Vol. 2(1) pp. 001-006.
Azuka O., Ayalokunrin M. & Orachu L., 2014, Portulaca oleracea (Purslane) plant – its nature and biomedical benefits, Internasional Jurnal of Boimedical Research, Nigeria.
Gandjar, I. Sjamsuridzal, W. Oetari, A. 2006. Mikologi. Yayasan Obor Indonesia, Jakarta. 39-41.
Hummel, C.S., Lu, C., Liu, J., Ghezzi, C.,Hirayama, B.A., Loo, D.D.F., Kepe, V.,Barrio, J.R., Wright, E.M., 2012. Structural selectivity of human SGLT inhibitors. Am. J. Physiol. Cell Physiol. 302, 373–382
IIDF, 2019. IDF Diabetes Atlas 2019. International Diabetes Federation.
International Diabetes Federation. (2017). IDF Diabetes Atlas 8th Edition
Kharwar, R.N., Verma, V.C., Strobel, G., and Ezra, D. 2008. The endophytic fungal complex of Catharanthus roseus (L.) G Don. Current Science. 95(2): 228-232.
Pahriyani, Ani., Priyo Wahyudi, Devi Marita, 2019.’ AktivitasInhibitor Α- Glukosidase Metabolit Fungi Endofit Yang Diisolasi Dari Rimpang Kunyit (Curcuma longa L.)‟. Majalah Farmasetika, 4 (Suppl1) 2019, 240 –245. https://doi.org/10.24198/mfarmasetika.v4i0.25888.
Paramitha, M.D. and Rahamanisa, S., 2016. Ekstrak etanol herba sambiloto (Andrographis paniculata) sebagai antidiabetik terhadap mencit wistar terinduksi aloksan. Majority, 5 (5), 75–79.
Prabavathy, D., Nachiyar, V.C. 2013. Antimicrobial and Antidiabetic Activity of An Endophytic Fungi Isolated from Adathoda beddomei.Int J Pharm Pharm Sci. (3): 780-783.
Rahimi VB, Ajam F, Rakshandeh H, 2019, A Pharmacological Review on Portulaca oleracea L.: Focusing on Anti-Inflammatory, Anti- Oxidant, Immuno-Modulatory and Antitumor Activities, Journal of Pharmacopuncture, Vol. 22, No. 1, pp.7-15 [online],
Subramanian, R., Asmawi, M.Z., and Sadikun, A., 2008. In vitro α- glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochimica Polonica, 55 (2), 391–398.
Sudha, P., Zinjarde, S.S., Bhargava, S.Y., dan Kumar, A.R. 2011. Potent α-Amilase Inhibitory Activity of India Ayuvedic Medicinal Plants. Complementary and Alternative Medicine. 44: 2278-2281.
Tan, R.X., and W. X. Zou. 2001. Endophytes: a rich source of functional metabolites. Nat.Prod. Rep. 18: 448–459
Yuniarto, A. and Selifiana, N., 2018. Aktivitas Inhibisi Enzim Alfa- glukosidase dari Ekstrak Rimpang Bangle (Zingiber cassumunar Roxb.) secara In vitro. Media Pharmaceutica Indonesiana (MPI), 2 (1), 22.
Zhou Yan-Xin, Hai-Liang Xin, Khalid Rahman, Su-Juan Wang, Cheng Peng & Hong Zhang, 2015, Portulaca oleracea L.: A Review of Phytochemistry and Pharmacological Effect, Hindawi Publishing Corporation, Biomed Research Internasional,
Zhang J, Onakpoya IJ, Pozaddzki P et al., 2015, The Safety of Herbal Medicine: From Prejudice to Evidence, Evidence-Based Complementary and Alternative Medicine, Vol.117, No. 3, pp.2-3 [online],
Published
2023-07-14
How to Cite
Siska, Suhaenah, A., & Latuconsina , S. I. S. (2023). The Potential of α- glucosidase Inhibition from Endophytic Fungi Associated in Portulaca oleracea L. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 6(1), 17-24. https://doi.org/10.24252/djps.v6i1.38049
Issue
Vol. 6 No. 1 (2023)
Section
Artikel

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