Perbandingan Metode Ekstraksi terhadap Kadar Flavonoid Total dan Aktivitas Antioksidan Batang Boehmeria virgata
Abstract
Penelitian ini bertujuan untuk membandingkan metode ekstraksi terhadap kandungan flavonoid total dan aktivitas antioksidan ekstrak batang Boehmeria virgata. Batang Boehmeria virgata diekstraksi melalui dua metode ekstraksi yaitu refluks dan maserasi. Kandungan flavonoid total ditentukan dengan menggunakan metode aluminium klorida dan dihitung sebagai ekivalen kuersetin (QE). Aktivitas antioksidan diukur dengan menggunakan spektrofotometer UV-VIS. Hasil penelitian menunjukkan aktivitas antioksidan (Uji DPPH) ekstrak Boehmeria virgata (Forst) Guill yang diperoleh melalui metode refluks lebih baik daripada dengan metode maserasi (IC50 pada 30,58 μg /mL) karena menunjukkan kandungan flavonoid lebih tinggi (sampel 2,554 mg QE / g).
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