Uji Diagnostik Jamur Dermatofita Pada Luka Kaki Penderita Diabetes Melitus dengan Metode PCR (Polymerase Chain Reaction)
Abstract
Copyright©2023
Diabetic wounds often occur in people with diabetes mellitus as a result of impaired tissue perfusion and fungal infections, one of which is excessive dermatophytes that cause tissue death. This study aims to detect dermatophyte fungi in foot wounds of diabetics with diabetes mellitus by the Polymerase Chain Reaction (PCR) method. The type of laboratory observational study with a cross sectional study design, and the sampling technique used is total sampling by swab foot wounds in diabetes mellitus patients. The research method uses the PCR method to detect the presence or absence of dermatophyte fungi characterized by the formation of DNA fragments on electrophoresis visualization. The results showed that from 8 samples detected, 5 samples or 62.5% were positive for dermatophyte fungal infection based on amplification results marked by the formation of an amplicon DNA target band measuring around 700-1000 bp using ITS1 primers (forward: 5' GGT TGG TTT CTT TTC CT 3') and ITS2 (reverse: 5' AAG TAA AAG TCG TAA CAA GG 3'), so it can be concluded that in identifying samples using the PCR (Polymerase Chain Reaction) method has a high sensitivity in detecting fungi so as to allow more specific and more accurate results.
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References
Adri, K., Arsin, A., & Thaha, R. M. (2020). Faktor Risiko Kasus Diabetes Mellitus Tipe 2 dengan Ulkus Diabetik di RSUD Kabupaten Sidrap. Jurnal Kesehatan Masyarakat Maritim, 3(1)101-108.https://doi.org/10.30597/jkmm.v3i1.10298
Anita, A. , A. N. N. , F. A. , M. N. (2020). Studi Literatur Identifikasi Jamur Pada Kuku dan Sela Kaki Penderita Diabetes Melitus. Journal Of Health Science & Technology, 1(2), 87–99. https://doi.org/10.53861/lontarariset.v1i2.75
Awaluddin, A. , Syarif, Rizalinda. , & Ilyas, Farida (2021). Penggunaan Metode Pcr – Rflp (Polymerase Chain Reaction – Restriction Fragment Length Polymorfism) Dalam Mendeteksi Jamur Dermatofit. Jurnal Media Kesehatan, 14, 96–102. https://doi.org/10.33088/jmk.v14i1.615
Dewanti, A. A. (2018). Hubungan Kontrol Gula Darah Dengan Kejadian Dermatofitosis Pada Pasien Dengan Riwayat Diabetes Melitus Di Rumah Sakit Umum Daerah Dr Moewardi Surakarta.Skripsi. Universitas Muhammadiyah Surakarta. http://eprints.ums.ac.id/id/eprint/60075
Eka Fitria, E. F., Abidah Nur, A. N., Nelly Marissa, N. M., & Nur Ramadhan, N. R. (2017). Karakteristik Ulkus Diabetikum pada Penderita Diabetes Mellitus di RSUD dr. Zainal Abidin dan RSUD Meuraxa Banda Aceh (Characteristics Of Ulcer Among Diabetes Mellitus Patient In Rsud Dr. Zainal Abidin and Rsud Meuraxa Banda Aceh). Buletin Penelitian Kesehatan, 45(3), 153–160. http://dx.doi.org/10.22435/bpk.v45i3.6818.153-160
Endrawati, D., Pribadi, E. S., Indrawati, A., & Kusumaningtyas, E. (2021). Penggunaan Teknik Molekuler untuk Mengenali Dermatofita yang Diisolasi dari Hewan Kesayangan di Jakarta dan Bogor. Jurnal Veteriner Maret, 22(1), 56–67. https://doi.org/10.19087/jveteriner.2021.22.1.56
Fridalni, N., Minropa, A., & Sapardi, V. S. (2019). Pengenalan Dini Penyakit Degeneratif. Jurnal Abdimas Saintika, 1(1), 129–135. http://dx.doi.org/10.30633/jas.v1i1.483
Gayatri, R. W. A. N. K. V. S. V. , A. P. S. (2019). Diabetes Melitus Dalam Era 4.0. Wineka Media.
Ghannoum, M., Mukherjee, P., Isham, N., Markinson, B., Rosso, J. del, & Leal, L. (2018). Examining the importance of laboratory and diagnostic testing when treating and diagnosing onychomycosis. International Journal of Dermatology, 57(2), 131–138. https://doi.org/10.1111/ijd.13690
Graceciela, Y. E. (2021). Hubungan Diabetes Melitus Tipe 2 Dengan Kejadian Dermatofitosis. Jurnal Kesehatan Masyarakat, 1(02 Desember), 22–26.
Husni, H., Asri, E., & Gustia, R. (2018). Identifikasi Dermatofita Pada Sisir Tukang Pangkas Di Kelurahan Jati Kota Padang. Jurnal Kesehatan Andalas, 7(3), 331–335. https://doi.org/10.25077/jka.v7i3.882
Kusnadi, J. , E. L. A. (2020). Polymerase Chain Reaction (PCR) Teknik dan Fungsi. UB Press.
Laowo, D. F., & Batubara, K. (2021). Pendidikan Kesehatan tentang Perawatan Luka Kaki pada Pasien Diabetes Mellitus Tipe 2. Jurnal Keperawatan Profesional (KEPO), 2(2), 68–73. https://doi.org/10.36590/kepo.v2i2.183
De Macedo Geisa Maria Campos, Samanta Nunes & Tania Barreto. 2016. Skin disorders in diabetes mellitus: an epidemiology and physiopathology review. Diabetology & Metabolic Syndrome. 8:63. https://doi.org/10.1186/s13098-016-0176-y
Masruroh, E. (2018). Hubungan umur dan status gizi dengan kadar gula darah penderita diabetes melitus tipe II. Jurnal Ilmu Kesehatan, 6(2). https://doi.org/10.32831/jik.v6i2.172
Nurfadillah, H. (2021). Identifikasi Jamur Dermatofita Penyebab Tinea unguium Pada Kuku kaki Petani di Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai. Kampurui Jurnal Kesehatan Masyarakat, 3(2), 84–92. https://doi.org/10.55340/kjkm.v3i2.498
Nurwahidah, S. Y. , T. T. (2018). Identifikasi Jenis Bakteri pada Luka Kaki Diabetik berdasarkan Penyebab Luka di Rumah Perawatan Luka dan Poliklinik Luka di Kota Makassar. Jurnal Kesehatan Manarang, 4(2), 97–103. https://doi.org/10.33490/jkm.v4i2.70
Pandapotan, A. (2018). Hubungan Antara Diabetes Mellitus dengan Terjadinya Dermatosis di Puskesmas Kejuruan Muda Kuala Simpang Kabupaten Aceh Tamiang. http://repositori.usu.ac.id/handle/123456789/7028
Pratiwi, P., Amatiria, G., & Yamin, M. (2016). Pengaruh stress terhadap kadar gula darah sewaktu pada pasien diabetes melitus yang menjalani hemodialisa. Jurnal Kesehatan, 5(1). http://dx.doi.org/10.26630/jk.v5i1.59
Primadani, A. F., & Nurrahmantika, D. (2021). Proses Penyembuhan Luka Kaki Diabetik Dengan Perawatan Luka Metode Moist Wound Healing. Ners Muda, 2(1), 9–16. https://doi.org/10.26714/nm.v2i1.6255
Saskia, T. I., & Mutiara, H. (2015). Infeksi jamur pada penderita diabetes mellitus. Jurnal Majority, 4(8), 69–74. https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/1476
Suryanti, S. (2021). Hubungan Gaya Hidup dan Pola Makan Dengan Kejadian Diabetes Mellitus di Rumah Sakit Bhayangkara Kota Makassar. Jurnal Promotif Preventif, 4(1), 1–9. https://doi.org/10.47650/jpp.v4i1.246
Susanti, D., & Pramana, Y. (2020). Hubungan antara efikasi diri dengan perawatan mandiri kaki pada pasien diabetes melitus di poli penyakit dalam rsud sultan syarif mohamad alkadrie pontianak. Tanjungpura Journal of Nursing Practice and Education, 2(1). http://dx.doi.org/10.26418/tjnpe.v2i1.41827
Tartor, Y. H., el Damaty, H. M., & Mahmmod, Y. S. (2016). Diagnostic performance of molecular and conventional methods for identification of dermatophyte species from clinically infected Arabian horses in Egypt. Veterinary Dermatology, 27(5), 401-e102. https://doi.org/10.1111/vde.12372
Velasquez-Agudelo, V., & Cardona-Arias, J. A. (2017). Meta-analysis of the utility of culture, biopsy, and direct KOH examination for the diagnosis of onychomycosis. BMC Infectious Diseases, 17(1), 1–11. https://doi.org/10.1186/s12879-017-2258-3
Welkriana, P. W., Saputra, A., & Susiwati, S. (2021). Identification of Dermatophite Fungi (Tinea Unguium) on Nail Screening of Chicken Traders in Panorama Market, Bengkulu City in 2021. Avicenna: Jurnal Ilmiah, 16(3), 120–128. https://doi.org/10.36085/avicenna.v16i3.2019
Widyasari, N. (2017). Hubungan karakteristik responden dengan resiko diabetes melitus dan dislipidemia kelurahan tanah kalikedinding. Jurnal Berkala Epidemiologi, 5(1), 130–141. https://pdfs.semanticscholar.org/7e1a/9e7164fe513249486d9bed1b69a3cab0b584.pdf
Yaner, N. R. (2019). Pengaruh Pendidikan Kesehatan Terhadap Peningkatan Pengetahuan Dan Sikap Penderita Diabetes Mellitus Dalam Pencegahan Luka Kaki Diabetik Di Puskesmas Jagir Kecamatan Wonokromo. NERSMID: Jurnal Keperawatan Dan Kebidanan, 2(2), 144–153. http://nersmid.unmerbaya.ac.id/index.php/nersmid/article/view/57
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