PERBANDINGAN KADAR ENDOTELIN 1 SEBAGAI INDIKATOR DISFUNGSI VASKULER PADA TIKUS WISTAR OBES DAN NO OBES
Abstrak
Peningkatan jumlah penderita obesitas pada beberapa tahun terakhir telah banyak memunculkan masalah di rana kesehatan. Penelitian ini bertujuan untuk melihat perbedaan kadar endothelin-1 (ET-1) pada tikus wistar obesitas dan non obesitas. Penelitian ini merupakan penelitian observasional dengan desain kontrol kasus. Sampel penelitian sebanyak dua belas ekor tikus wistar yang di ambi dari perternakan abdi jaya Yogyakarta yang berumur 150-180 hari. Sampel dibagi menjadi dua kelompok, yaitu kelompok obes dan kelompok non obes yang terdiri atas enam sampel tikus obes yang diberi pakan diet tinggi karbohidrat dan tinggi lemak sedangkan enam tikus yang linnya diberi pakan biasa. Pengukuran kadar ET-1 dilakukan dengan menggunakan ELISA. Analisis data dilakukan dengan uji independen T-sampel. Hasil penelitian menunjukkan terdapat perbedaan significant kadar ET-1 pada tikus obes dengan tikus non obes (p=0,01). Dengan kata lain, kadar ET-1 dapat dijadikan sebagai sebagai indicator disfunggsi vaskuler pada keadaan obesitas.
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Referensi
Bairwa, S. C., et al. 2016. Cardiomyocyte Antihypertrophic Effect of Adipose Tissue Conditioned Medium from Rats and Its Abrogation by Obesity is Mediated by the Leptin to Adiponectin Ratio. PLoS One, 11, e0145992.
Billy, M., et al. 2016. Obesity as Predictor of Mortality of Colorectal Cancer: an Evidence-based Case Report. Acta Med Indones, 48, 242-246.
Brian, R. W., et al. 2011. Enhanced endothelin 1 system activity with overweight and obesity. Am J Physiol Heart Circ Physiol, 301, H689–H695.
Campia, U., et al. 2014. The vascular endothelin system in obesity and type 2 diabetes: pathophysiology and therapeutic implications. Life Sci, 118, 149-55.
De Ciuceis, C., et al. 2011. Effects of weight loss on structural and functional alterations of subcutaneous small arteries in obese patients. Hypertension, 58, 29-36.
De Miguel, C., et al. 2017. Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney. Sci Rep, 7, 43152.
Engin, A. 2017. Endothelial Dysfunction in Obesity. Adv Exp Med Biol, 960, 345-379.
Finucane, M. M., et al. 2011. National, regional, and global trends in body mass index since 1980: Systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9.1 million participants. Lancet, 377, 557-67.
Frisbee, J. C. 2007. Obesity, insulin resistance, and microvessel density. Microcirculation, 14, 289-98.
Goudie, M. J., et al. 2016. Characterization and in vivo performance of nitric oxide-releasing extracorporeal circuits in a feline model of thrombogenicity. J Biomed Mater Res A.
Grassi, G., et al. 2010. Structural and functional alterations of subcutaneous small resistance arteries in severe human obesity. Obesity (Silver Spring), 18, 92-8.
Houde, M., et al. 2016. Endothelin-1: Biosynthesis, Signaling and Vasoreactivity. Adv Pharmacol, 77, 143-75.
Jiang, J., et al. 2014. Does Obesity Affect the Surgical Outcome and Complication Rates of Spinal Surgery? A Meta-analysis. Clin Orthop Relat Res, 472, 968-75.
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