STUDI AKTIVITAS INHIBITOR TIROSINASE DAN KADAR FENOLIK TOTAL EKSTRAK METANOL JAMUR TIRAM PUTIH (Pleurotus ostreatus)

  • Nur Syamsi Dhuha UIN Alauddin Makassar
  • Nur Suci Suci Wahdaniya
  • Syamsuri Syakri UIN Alauddin Makassar

Abstract

Oyster mushroom (Pleurotus ostreatus) contains vitamin B, vitamin C and carbohydrates such as β-glucans, monoterpenoids, triterpenoids, potential sources of lignin and phenols. The presence of phenolic compounds indicates the ability of oyster mushrooms as potential antioxidants and anti-tyrosinases. Searching for depigmentation agents in cosmetics is necessary to ensure safety and reduce side effects. The aims of this study are to determine the inhibitory activity of tyrosinase and total phenolic levels of Pleurotus ostreatus methanol extract. Tyrosinase inhibitor activity was measured using the Enzyme Linked Immunosorbent Assay (ELISA) method at 490 nm with various concentrations, which were 55 ppm, 65 ppm, 75 ppm, 85 ppm and 95 ppm. Determination of total phenolic levels at a concentration of 100 ppm using a UV-Vis Spectrophotometer at 744.8 nm. Based on the research, the percentage of tyrosinase inhibitor of Pleurotus ostreatus methanol extracts at concentrations of 55 ppm, 65 ppm, 75 ppm, 85 ppm and 95 ppm were 17.6%, 19.5%, 22.2%, 25.9%, and 35.7%, respectively. The result of the IC50 and total phenolic content are 9.06% and 1.13%. These results exhibit that the methanol extract of Pleurotus ostreatus has a very strong tyrosinase inhibitor power.

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Author Biography

Nur Syamsi Dhuha, UIN Alauddin Makassar
Phamacochemistry

References

Apsari Pramudita Dwi., a. S. Penetepan Kadar Fenolik Total Bunga Rosella Merah (Hibiscus sabdariffa L.) dengan Variasi Tempat Tumbuh secara Spektrofotometri. Jurnal ilmiah Kefarmasian. 2011

Chrinang P., I. K. Amino Acids and Antioxidant Properties of the Oyster Mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju. Science Asia Journal. 2009

Ezzedine K, G. C. Expatriates in high- UV index and tropical countries: sun exposure and protection behavior in 9,416 French adults. Journal of Travel Medicine. 2007

Fawole A, M. N. Antibacterial, Antioxidant and Tyrosinase Inhibitor Activities of Pomegranate Fruit Peel Methanolic Extract. BMC Complement & Altern Med. 2012

Fountoulakis. Removal of Phenolics in Olive Mill Wastewater Using the White-rot Fungus Pleurotus ostreatus. Water Res. 2007

Iwalokun B. A., u. U. Comparative Phytochemical Evaluation, Antimicrobial and Antioxidant Properties of Pleurotus ostreatus. African Journal of Biotechnology. 2007

Jeong – H. K, S. –J.–R.–I.–C.–C.–J.–C. The Different Antioxidant and Anticancer Activites depending on the Color of Oyster Mushroom. Journal of Medicinal Plant Research. 2009

Miller, A. L. Antioxidant Flavonoids Structure, Function and Clinical Usange. ALT Med Rev. 1996

Sanjeewa KKA, K. E. Bioactive properties and potentials cosmeceutical applications of phlorotannins isolated from brown seaweeds: a review. Journal of Photochemistry and Photobiology B: Biology. 2016

Sheng, C. T. Natural Melanogenesis Inhibitor Acting Through the Down Regulation of Tyrosinase Activity. 2012

Smit N, V. J. The hunt for natural skin whitening agents. International Journal of Molecular Sciences. 2009

Tjokrokusumo. Jamur Tiram (Pleurotus ostreatus) untuk Meningkatkan Ketahanan Pangan dan Rehabilitasi Lingkungan. JRL. 2008

WorldHealthOrganization. Global Solar UV index. A Practical Guide. 2002

Zeo Draelos, D. A. Dyspgmentation, Skin Physiology, And a Novel Approach to Skin Lightening. Journal of Dermatology. 2013

Published
2020-12-25
Abstract viewed = 721 times