PENGARUH PEMBERIAN SPIRULINA PERORAL YANG DIBERI CCL4 TERHADAP KADAR UREUM DAN KREATININ DARAH TIKUS PUTIH

  • Swandito Wicaksono Universitas Bengkulu
    (ID)

Abstract

Free radical is Carbon Tetra Chloride, which is organic toxic solution through liver, heart, and kidneys. Spirulina is green-blue algae which photosynthetic microalgae, spiral-shape, multicelullar and chemic-riched and able to correct body’s damage by free radicals, for instance Beta Carotene, amino acids, Gamma Linolenic Acid (GLA). The aims of this research is to discover whether Spirulina can correct kidneys function from CCl4-induction on white rats (Rattus norvegicus) with weight ±300 gram. Decreasing kidneys fungtion was evaluated by increasing blood ureum and creatinine levels. ANOVA analysis performed that CCl4 effect blood ureum and creatinin (p<0,05). Spirulina at dose 3,471 ml/300gr body weight was the most effective through correction kidneys function.

ABSTRAK

Radikal bebas Carbon Tetra Chloride merupakan pelarut organik yang bersifat toksik terhadap hati, jantung, dan ginjal. Spirulina adalah alga hijau-biru yang merupakan mikroalga fotosintetik, berbentuk spiral, multiseluler dan kaya senyawa kimia yang dapat memperbaiki berbagai kerusakan di dalam tubuh yang disebabkan karena radikal bebas, seperti Beta Carotene, Asam Amino, Gamma Linolenic Acid (GLA). Penelitian ini bertujuan untuk mengetahui efek dari Spirulina yang dapat memperbaiki kerusakan fungsi ginjal akibat induksi CCl4 pada tikus putih dilihat dari kadar ureum dan kreatinin darah. Penurunan fungsi ginjal terjadi pada hari pertama setelah aklimatisasi. Penurunan fungsi ginjal dapat dilihat dari kenaikan kadar ureum dan kreatinin darah. Hasil uji ANOVA pemberian CCl4 berpengaruh terhadap kenaikan ureum dan kreatinin darah (p<0,05). Spirulina 3,471 ml /300grBB merupakan dosis paling efektif dalam memperbaiki fungsi ginjal.

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Author Biography

Swandito Wicaksono, Universitas Bengkulu
Departemen Fisiologi

References

Wijaya A. Radikal Bebas Dan Parameter Status Antioksidan. Bandung: Lab Klinik Prodia; 1996.

Tang D, Hu S, Dai F, et al. Self-Templated Synthesis of Mesoporous Carbon from Carbon Tetrachloride Precursor for Supercapacitor Electrodes. ACS Applied Materials and Interfaces. 2016;8(11):6779-6783. doi:10.1021/acsami.5b12164

Suzek H, Celik I, Dogan A, Yildirim S. Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharmaceutical Biology. 2016;54(3):451-457. doi:10.3109/13880209.2015.1045086

Scholten D, Trebicka J, Liedtke C, Weiskirchen R. The carbon tetrachloride model in mice. Laboratory Animals. 2015;49:4-11. doi:10.1177/0023677215571192

Yilmaz-Ozden T, Can A, Karatug A, Pala-Kara Z, Okyar A, Bolkent S. Carbon tetrachloride-induced kidney damage and protective effect of Amaranthus lividus L. in rats. Toxicology and Industrial Health. 2014;32(6):1143-1152. doi:10.1177/0748233714555390

Rajendran N, SR SK, K TM, P J. Biochemical evaluation of creatinine and urea in patients with renal failure undergoing hemodialysis. Journal of Clinical Pathology and Laboratory Medicine. 2017;1(2):1-5.

Henrikson R, Yoro C, Ruppert R. Fifth Printing, Revised Edition Online.; 1989.

La RDE, Ciencias FDE. Sp/Rulina (Arthrospira) an Edible Microorganism a Review. Universitas Scientiarum. 2003;8(1):7-24.

Kirschweng B, Tátraaljai D, Földes E, Pukánszky B. Natural antioxidants as stabilizers for polymers. Polymer Degradation and Stability. 2017;145:25-40. doi:10.1016/j.polymdegradstab.2017.07.012

Chowdhury MRH, Sagor MAT, Tabassum N, Potol MA, Hossain H, Alam MA. Supplementation of Citrus maxima Peel Powder Prevented Oxidative Stress, Fibrosis, and Hepatic Damage in Carbon Tetrachloride (CCl4) Treated Rats. Evidence-based Complementary and Alternative Medicine. 2015;2015. doi:10.1155/2015/598179

Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry. 2015;97:55-74. doi:10.1016/j.ejmech.2015.04.040

Published
2019-10-28
Abstract viewed = 829 times