IDENTIFIKASI PROTEIN DARI CRUDE ANTIGEN OUTER MEMBRANE PROTEIN (OMP) SALMONELLA ENTERICA SEROVAR TYPHI ASAL SUSPEK DEMAM TIFOID MAKASSAR
Abstract
Penelitian ini bertujuan untuk mengetahui bobot molekul protein OMP S. Typhi
asal Makassar dengan menggunakan metode SDS-PAGE. Penelitian ini dilakukan
pada bulan Mei sampai Juni 2014 di Laboratorium Imunologi dan Biologi
Molekuler Fakultas Kedokteran. Penelitian ini menggunakan antigen OMP hasil
ekstraksi kultur bakteri S. Typhi dari serum darah penderita demam tifoid yang
diperoleh dari Rumah Sakit di Kota Makassar. Monitoring bobot molekul
dikerjakan menggunakan SDS-PAGE metode Laemmli yang telah dimodifikasi
yaitu separating gel 12%, stacking gel 3%, dan pewarnaan gel (staining)
menggunakan coomassie blue. Proses elektroforesis menggunakan tegangan
listrik 120 volt 40 mA selama 3,5 jam. Gel hasil SDS-PAGE memperlihatkan
adanya tiga band protein mayor OMP S. Thyphi yaitu pada 25 kda, 36 kda, 55
kda, dikarenakan pada ekstrak kasar masih banyak debris dari protein lain
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References
Muliawan, dan Surjawidjaya. 1999. Diagnosis dini demam tifoid dengan menggunakan protein membran luar S. Typhi sebagai antigen spesifik.
Sarasombath S, Korbsrisate S, Banchuin N, Thanomsakyuth A, Sukosol T, Ekpo P. 1998. Serological approaches of Typhoid Fever. Med.Indon: vol. 7,54 -8.
Brooks, Geo, F. Janet, S. Butel & L.Nicholas O., 2005. Mikrobiologi Kedokteran EGC. Jakarta.
Diana Chusna Mufida, Candra Bumi, dan Heni Fatmawati. 2009. Peran Protein Membran Luar 55 Kda Salmonella Typhi Isolat Jember Sebagai Protein Hemaglutinin Dan Adhesin. Berk. Penel. Hayati: 15 (11–16), 2009
Winarsih, Sri. 2007. Cloning and expression gene Adhesin Adho36 of S.Typhi as candidate vaccines of Typhoid fever. Report of Research. RISTEK
Aslam MS, Akhter M, Rasheed R, Samra ZQ, Gull I, Athar MA, 2012. Identification and purification of antigenic 34 kDa outer membrane protein of Salmonella typhi. Pubmed. Clin Lab.; 58 (9-10): 1071-7
Kaur, J, and Jain, S.K. 2013. High Level Expression of 49kDa Outer Membrane Protein of Salmonella enterica serovar Typhi. Annals of Biological Research, 4 (1): 107- 117.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Scopes, K.R. 1994. Protein Purification. Springer Scienses.
Wilson K. 1994. Protein and enzyme techniques In Practical Biochemistry, (ed. Wilson K and Walker JM), Cambridge University Press. p.161‐226.
Smith BJ.1984. SDS polyacrylamide gel electrophoresis of proteins. Di dalam Walker JM, editor. Proteins. Methoda in Molecular Biology. Volume ke-1. Clifton:Humana Pr.hlm 41-55.)
Sumarmo S, Garna H, Sri RSH, Hindra IS. Buku Ajar Infeksi dan Pediatrik Tropis. Jakarta: Badan Penerbit IDAI; 2002.
Supriyadi. 2006. Keanekaragaman Genetik Populasi Wereng Hijau, Nephotettix virescens Asal Wilayah Endemi dan Nonendemi Virus Tungro Padi. Disertasi. Universitas Gajah Mada Yogyakarta.
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